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Novel application of alpha-mangostin in prevention and treatment of hyperuricemia and gout

A technology of hyperuricemia and mangostin, which is applied in the field of medicine, can solve the problems of gout and hyperuricemia without seeing it, and achieve the effect of strong activity

Inactive Publication Date: 2018-11-13
KUNMING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that α-mangostin has a wide range of pharmacological effects, including inhibiting cyclooxygenase and glucosidase, antagonizing histamine receptors, anti-inflammation, anti-oxidation, anti-pathogenic bacteria and fungi, inhibiting sphingomyelinase, Promote apoptosis and anti-Alzheimer's disease [5] , but there is no report on the treatment of gout and hyperuricemia

Method used

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  • Novel application of alpha-mangostin in prevention and treatment of hyperuricemia and gout
  • Novel application of alpha-mangostin in prevention and treatment of hyperuricemia and gout
  • Novel application of alpha-mangostin in prevention and treatment of hyperuricemia and gout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The dose-effect relationship of the inventive compound in reducing blood uric acid in mice with hyperuricemia induced by potassium oxonate

[0025] Healthy male Kunming mice (body weight 18-22g) were provided by Beijing Huafukang Biotechnology Co., Ltd., certificate number: SCXK (Beijing) 2014-0004. Animals were randomly divided into normal control group, hyperuricemia model group, inventive compound 1.0, 2.0 and 4.0 mg / kg dosage groups, febuxostat 1.0 mg / kg group mg / kg. The test compound was formulated with 0.5% sodium carboxymethylcellulose (0.5% CMC-Na) to an appropriate concentration, administered orally, twice a day, 5 doses in total. Reference [Hall IH, Scoville JP, Reynolds DJ, Simlot R, Duncan P. Substituted cyclic imides aspotencial anti-gout agents. Life Sciences. 1990, 46: 923-1927.Stavric B, ClaymanS, Gradd REA, Hebert D.Some in vivo effects in the rat induced by chlorprothixene and potassium oxonate Pharmacology Research, 1975, 7: 117-124] using the uricas...

Embodiment 2

[0032] The time-effect relationship of the inventive compound in reducing blood uric acid in mice with hyperuricemia induced by potassium oxonate

[0033]Healthy male Kunming mice (body weight 18-22g) were provided by Beijing Huafukang Biotechnology Co., Ltd., certificate number: SCXK (Beijing) 2014-0004. The animals were randomly divided into 10 groups, 10 animals in each group, which were normal group, hyperuricemia model group, 1, 2, 4, 6h groups administered with 4.0 mg / kg of the inventive compound, and febuxostat 1.0 mg / kg administered 1, 2, 4, 6h groups. The administration volume was 10ml / kg, and the administration was once. One hour before intragastric administration, 2 hours after intraperitoneal injection of potassium oxonate 500mg / kg (20ml / kg), blood was taken from the orbital vein, centrifuged at 3600rpm / min for 10min, the supernatant was taken, and the blood uric acid level was measured by the phosphotungstic acid method. The results are shown in the table 2.

...

Embodiment 3

[0040] Effects of Invented Compounds on the Activities of Xanthine Oxidase (XOD) and Xanthine Dehydrogenase (XDH) in Vivo

[0041] Get example 1 animal serum 100 μ l and the liver of example 2 animal according to mass: volume=1: 9, make 10% liver homogenate with sonicator under ice-bath condition, 3600rpm / min 4 ℃ centrifugation 10min, get liver homogenate Serum and liver homogenates were used to measure XOD activity in 100 μl of serum according to the instructions of the xanthine oxidase kit. Serum and liver XOD activities were calculated according to the formula.

[0042] Serum XOD activity (U / L) = (measured OD value - blank OD value) × total reaction volume × 1 ÷ (12.6 × 10 -3 × sampling amount × 1 × reaction time 20min);

[0043] Liver XOD activity (U / gprot) = (measured OD value - blank OD value) × total reaction volume × 1 ÷ (12.6 × 10 -3 × sample volume × 1 × reaction time 20min) ÷ protein concentration (g.prot / L)

[0044] Another 20 μl of animal serum from Example 1 ...

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Abstract

The invention relates to novel application of alpha-mangostin in the prevention and treatment of hyperuricemia and gout. Researches show that the alpha-mangostin is high in uric acid lowering effect,the serum uric acid level of a oteracil-potassium-induced or uric-acid-induced hyperuricemia mouse can be evidently lowered by feeding 2.0mg / kg of the alpha-mangostin into the mouse through gavage, the alpha-mangostin is a novel uricosuric drug, the titer of the alpha-mangostin is higher than the premium uricosuric drug benzbromarone, the alpha-mangostin is also low in toxicity, the orally-taken maximum tolerance dose of the mouse is 5g / kg, and the alpha-mangostin can be used for preparing orally-taken drugs and healthcare products for resisting the gout, the hyperuricemia and diseases relatedto the hyperuricemia.

Description

technical field [0001] The invention relates to the new application of α-mangostin in the prevention and treatment of hyperuricemia and gout, and belongs to the technical field of medicine. Background technique [0002] Gout is a group of metabolic diseases caused by the excessive production of uric acid due to purine metabolism disorder and / or the decrease of uric acid excretion, which leads to the increase of uric acid level and the formation of uric acid crystals deposited in joints, soft tissues, and kidneys. Uric acid is the end product of human purine metabolism, and hyperuricemia is usually considered a sign of gout. According to statistics, about 5% to 12% of patients with hyperuricemia will eventually develop gout [1] . In Europe, North America, Japan and other regions and countries, the incidence of gout is relatively high, but with the improvement of people's living standards in our country, the extension of life span, the change of diet structure, and the increa...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P19/06
CPCA61K31/352
Inventor 李玲周志宏牛艳芬高丽辉林华李强
Owner KUNMING MEDICAL UNIVERSITY
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