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Method for screening bacterial strain for authenticating antagonistic aspergillus flavus

A technology of Aspergillus flavus and antagonistic bacteria, which is applied in the field of strain screening and identification, and can solve problems such as chemical residues

Inactive Publication Date: 2018-11-13
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Common physical and chemical methods have a great effect on the inhibition of Aspergillus flavus and the removal of toxins, but they will cause many problems, such as chemical residues, which have attracted more and more attention

Method used

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  • Method for screening bacterial strain for authenticating antagonistic aspergillus flavus
  • Method for screening bacterial strain for authenticating antagonistic aspergillus flavus
  • Method for screening bacterial strain for authenticating antagonistic aspergillus flavus

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Embodiment

[0031] A method for screening and identifying bacterial strains resistant to Aspergillus flavus, comprising the following steps:

[0032] (1) Preparation of Aspergillus flavus spore suspension

[0033] Inoculate the toxin-producing strain of Aspergillus flavus on the high-salt Chase's slant test tube culture medium, culture it in a constant temperature medium at 37°C for 72-96 hours, wait for the spores to fully form, add 4mL of sterilized water, and scrape off the Aspergillus flavus spores in the test tube , poured into a triangular flask with sterilized water, and placed in a shaker to disperse the spores, filtered the dispersed spores with gauze to make a suspension of Aspergillus flavus spores, counted with a hemocytometer, and suspended the spores of Aspergillus flavus Adjust the liquid concentration to 10 7 cfu / mL, the Aspergillus flavus spore suspension should be prepared and used immediately;

[0034] (2) Primary screening of antagonistic bacteria

[0035] Quickly p...

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Abstract

The invention discloses a method for screening a bacterial strain for authenticating antagonistic aspergillus flavus. The method comprises the steps of aspergillus flavus spore suspension preparation,antagonistic bacterium preliminary screening, antagonistic bacterium secondary screening to obtain an antagonistic bacterium. The antagonistic bacterium is authenticated through morphological feature, physiological and biochemical feature, 16SrDNA and gyrB gene sequence analysis; the bacterial strain B10 with obvious inhibition on the aspergillus flavus is screened; through authentication, the bacterial strain B10 is bacillus amyloliquefaciens. A novel method and theoretical basis are provided for preventing and controlling aflatoxin B1 fungaltoxin poisoning. The method has important practical significance of production.

Description

technical field [0001] The technical field of bacterial strain screening and identification of the present invention particularly relates to a method for screening and identifying bacterial strains that resist Aspergillus flavus. Background technique [0002] Aspergillus flavus belongs to the genus Aspergillus, which pollutes a variety of important plants and plant products, and poses a threat to the health of humans and animals. Aspergillus flavus can cause aspergillosis in humans, and its hazard rate is second only to Aspergillus fumigatus. Adekoya et al. found the highest incidence of Aspergillus flavus in corn wine contaminated with mycotoxins, which was 26%. Aspergillosis caused by Aspergillus flavus infection is becoming more and more common. It mainly infects the lungs, nails, spinal cord and other organs of patients, leading to nerve paralysis. The secondary metabolites produced by Aspergillus flavus are called aflatoxins. Aflatoxins exhibit acute and chronic toxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/6895C12Q1/04C12R1/67
CPCC12N1/14C12Q1/6895
Inventor 龙淼党萌何剑斌李鹏杨淑华曹宇王楠
Owner SHENYANG AGRI UNIV
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