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Cadmium chelate type immune complex with excellent effect and preparation method of immune complex

An immune complex and chelation type technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problem that the preparation method of cadmium chelation type immune complex is not perfect, the preparation period of the target object is prolonged, and the scope of application is not enough. It can reduce the production cycle, increase the market competitiveness, and achieve the effect of a wide range of applications.

Inactive Publication Date: 2018-11-16
广东腾湃医疗股份有限公司
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  • Claims
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Problems solved by technology

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing cadmium chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid. If the ions and other wastes in the waste liquid are not treated up to the standard, they will cause chemical pollution.

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  • Cadmium chelate type immune complex with excellent effect and preparation method of immune complex

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preparation example Construction

[0033] A preparation method of the cadmium chelated immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: First prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0035] S2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0036] S3: Add the prepared chelating agent solution to the carrier protein solution, place it in a constant temperature water bath and stir evenly, and keep the constant temperature water bath for 24 hours to obtain the mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO to the dialysis bag 3 Boil the solution, fix the dialysis bag in the middle of the tank, and add enough ddH to the tank 2 O, the ddH 2 The volume ratio of the volume of O to the solution in the dialysis bag is 85:1, repeat this step 2 times, put the mixture in S...

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Abstract

The invention discloses a cadmium chelate type immune complex with excellent effect. The cadmium chelate type immune complex refers to one of the following complexes: a complex formed from cadmium ions by binding with an immune complex; a complex formed from the cadmium ions and an antibody specifically binding with a carrier protein after the cadmium ions bind with the carrier protein; a complexformed from the cadmium ions by binding with an immune globulin and a carrier protein sequentially. The invention further discloses a preparation method of the cadmium chelate type immune complex withthe excellent effect. The method comprises the following steps: S1, preparing a chelating agent solution; S2, preparing a carrier protein solution; S3, performing stirring overnight; S4, performing dialysis treatment; S5, adding the cadmium ions; S6, recovering waste liquor; S7, performing specific binding. The method is wider in application range, cost can be saved, dialysis rate is increased, preparation period can be shortened, the method further has the characteristics of being energy-saving and environmentally friendly, chemical pollution is avoided, and the environmental protection effect is good.

Description

technical field [0001] The invention relates to the field of chelates, in particular to a cadmium chelating immune complex with excellent effects and a preparation method thereof. Background technique [0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing cadmium chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/68
CPCG01N33/531G01N33/6848
Inventor 张积仁陈振展傅世林常承瑜张园园王兴国
Owner 广东腾湃医疗股份有限公司
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