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Aflatoxin pretreatment kit applying immunomagnetic bead technology

A technology of aflatoxin and immune magnetic beads, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low separation efficiency and complicated purification and separation operations, so as to improve the accuracy and precision and reduce the interference of background substances , the effect of easy operation

Pending Publication Date: 2018-11-16
沭阳康源泰博生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to make up for the deficiencies in the prior art, to provide a kind of aflatoxin sample pretreatment kit for immunomagnetic beads, which is used for the enrichment and purification of aflatoxin in samples, so as to solve the problem of aflatoxin sample Technical problems such as complicated sample purification and separation operations and low separation efficiency

Method used

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  • Aflatoxin pretreatment kit applying immunomagnetic bead technology

Examples

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Embodiment 1

[0032] 1. Preparation of anti-aflatoxin antibody immunomagnetic beads:

[0033] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time with an interval of 3 minutes, remove the activation buffer, add 200 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0034] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add aflatoxin antibody, mix well, and incubate at 37°C for 3 hours to prepare aflatoxin antibody immune magnetic beads;

[0035] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0036] 2. The application of anti-aflatoxin immunomagnetic beads to the enrichment of aflatoxin in wheat samples:

[0037] 1. Weigh 5.0 g of wh...

Embodiment 2

[0040] 1. Preparation of anti-aflatoxin antibody immunomagnetic beads:

[0041] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times with an interval of 5 minutes each time, remove the activation buffer, add 180 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0042] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add aflatoxin antibody, mix well, and incubate at 37°C for 3 hours to prepare aflatoxin antibody immune magnetic beads;

[0043] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0044]2. The application of anti-aflatoxin immune magnetic beads to the enrichment of aflatoxin in feed samples:

[0045] 1. Weigh 2.0 g of crus...

Embodiment 3

[0048] 1. Preparation of anti-aflatoxin antibody immunomagnetic beads:

[0049] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time at an interval of 4 minutes, remove the activation buffer, add 250 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 4 hours , to activate the magnetic beads;

[0050] 2. Wash the magnetic beads with coupling buffer three times with an interval of 4 minutes each time, add aflatoxin antibody, mix well, and incubate at 37°C for 4 hours to prepare aflatoxin antibody immune magnetic beads;

[0051] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 4 minutes between each time. Incubate with blocking solution at 37°C for 4 hours, store in storage buffer at 4°C until use;

[0052] 2. The application of anti-aflatoxin immunomagnetic beads to the enrichment of aflatoxin in corn samples:

[0053] 1. Crush the corn sample...

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Abstract

The invention provides preparation and application for an aflatoxin pretreatment kit applying an immunomagnetic bead technology. The kit is mainly composed of a sample bin, a reagent bin, a cleaning bin, an elution bin and the like. According to the preparation and application for the aflatoxin pretreatment kit applying the immunomagnetic bead technology, the binding surface area is expanded through the enrichment effect of immunomagnetic beads and the sufficient diffusion of the immunomagnetic beads in liquid, the immune response is more thorough, the kit can be widely applied to enrichment and separation of aflatoxin in products such as feed, grain oil products, so that the detection sensitivity can be improved, and the phenomenon of missing detection is avoided; and meanwhile, the use of a large amount of organic solvent is reduced.

Description

technical field [0001] The invention belongs to the field of food safety detection and analysis, in particular to an aflatoxin pretreatment kit using immunomagnetic bead technology. Background technique [0002] Aflatoxins are a class of compounds with similar structures. Aflatoxins in nature are mainly secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. There are more than 20 kinds of aflatoxins discovered so far, among which aflatoxins B1, B2, G1, G2 and M1 are the most common. Aflatoxins are classified as Class I carcinogens by the World Health Organization, and aflatoxin B1 is the most toxic, which is 68 times more toxic than arsenic. Aflatoxins can damage the liver tissue of humans and animals, and in severe cases can lead to liver cancer or even death. [0003] Due to the complexity of biological components, low concentration of analytes, complex pretreatment process and other factors, the existing aflatoxin extraction process is not sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/531G01N33/54326G01N33/5436
Inventor 张波张学记郗日沫王鹏雷少军张二林雷达刘向阳孙骁帅
Owner 沭阳康源泰博生物科技有限公司
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