Application method of biological bacterial fertilizer for improving saffron yield in cultivation

Abstract

The invention discloses an application method of a biological bacterial fertilizer for improving the saffron yield in cultivation. The application method comprises the following steps: step S101: preparing materials, preparing a plurality of endophytic fungi, and placing the endophytic fungi on the inside of a slant test tube; step S102: transferring the endophytic fungi to a PDA medium after activation of the endophytic fungi from the slant test tube preserved at low temperature, and culturing for 10-20 days; step S103: punching the endophytic fungi into a bacterium cake of 5 mm with a puncher; step S104: inoculating the bacterium cake into the PDB medium at sterilized normal temperature for fermentation and cultivation; step S105: sealing, placing in a shaker, and culturing for 7 days; step S106: mixing the prepared endophytic fungi with tap water. According to the invention, a target strain with enhanced resistance is screened out from the endophytic fungi of saffron, and the liquidbiological bacterial fertilizer is prepared and is applied to the cultivation of saffron; the yield and the active ingredient content of saffron can be greatly increased by spraying the biological bacterial fertilizer; the fertilization method of the technology is simple, easy to promote in a large area and small in investment; and the saffron produced is a green product.

Description

technical field [0001] The invention relates to the field of application methods of biological bacterial fertilizers in cultivation, in particular to an application method of biological bacterial fertilizers in cultivation to improve the yield of saffron. Background technique [0002] This technology relies on a part of the National Natural Science Foundation of China project "Effects of saffron endophytic fungi on the degradation of host bulbs, salt tolerance and quality of medicinal materials and their cultivation application". In order to construct the quality evaluation system of saffron medicinal materials, study the population structure of endophytic fungi in saffron, screen the target strains for growth promotion and resistance enhancement, and investigate the effect of target bacteria on the quality of saffron medicinal materials in artificial climate chamber and experimental field. influences. It is hoped that through this study, the resources and functions of endo...

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Problems solved by technology

Saffron is managed manually from planting and cultivation to harvesting and processing. The production area is narrow, the scale is small, a lot of labor and financial resources are consumed, the output is low, and it is difficult to meet the needs of all aspects. This is the main reason why its price has been high reason

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