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Test strip for detecting methylamphetamine and preparation method and application of test strip

A technology for methamphetamine and test strips, applied in the field of methamphetamine detection, can solve the problems of inability to realize rapid detection of large batches of samples, complicated operation, long time-consuming, etc. Save simple effects

Inactive Publication Date: 2018-11-20
郑州左安检测科技有限公司
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the existing methods for detecting methamphetamine, which are complex in operation, time-consuming, and unable to realize the characteristics of rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, and fast detection speed. Fast and low-cost test strip for detecting methamphetamine and its preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of methamphetamine samples

Method used

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  • Test strip for detecting methylamphetamine and preparation method and application of test strip
  • Test strip for detecting methylamphetamine and preparation method and application of test strip
  • Test strip for detecting methylamphetamine and preparation method and application of test strip

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preparation example Construction

[0042] The preparation method of the test strip for detecting methamphetamine mainly comprises the following steps:

[0043] 1) Label the methamphetamine antibody with fluorescent microspheres and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with fluorescent microsphere-labeled methamphetamine antibody;

[0044] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the detection line (T) and quality control line (C) to prepare methamphetamine hapten-carrier protein The detection line of the conjugate and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0045] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0046] The following step-by-step detailed description:

[0047] 1. Preparation of fluorescent mi...

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Abstract

The invention discloses a test strip for detecting methylamphetamine and a preparation method and an application method of the test strip. The test strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of methylamphetamine can be achieved. The invention further provides a method for detecting methylamphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale methylamphetamine samples can be achieved.

Description

technical field [0001] The invention relates to the detection of methamphetamine, in particular to a test strip for quantitatively detecting methamphetamine by using time-resolved fluorescent microsphere immunochromatography technology and a preparation method and an application method thereof. Background technique [0002] Methamphetamine, commonly known as "ice", is a stimulant psychotropic drug with a strong central nervous system excitatory effect. It is a class of psychotropic drugs and narcotic drugs strictly controlled by the state. At present, the abuse of amphetamine-like substances in the world is on the rise. Experts predict that amphetamine-type stimulants will become one of the most widely abused drugs of the 21st century. [0003] As criminal cases related to methamphetamine increase year by year and the country's management of methamphetamine becomes increasingly strict, this field puts forward higher requirements for the detection of methamphetamine and meth...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/577G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/577G01N33/946
Inventor 贾嘉张振兴吕小翠赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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