Tubular plasma circulating nucleic acid extraction method and system thereof

Abstract

The invention discloses a tubular plasma circulating nucleic acid extraction method and a system thereof. The extraction system comprises a sample adding cavity, a pressing mechanism, one or more washing liquid storage cavity, an elution liquid storage cavity and touched liquid leaking mechanisms, wherein the sample adding cavity is capable of containing mixed and cracked samples; an adsorption mechanism is arranged in the sample adding cavity and can be combined with circulating nucleic acid in the mixed and cracked samples; the pressing mechanism is capable of moving down in the sample adding cavity; the washing liquid storage cavity is capable of containing washing buffer liquid; the elution liquid storage cavity is capable of containing elution liquid and is arranged under the washingliquid storage cavity; the touched liquid leaking mechanisms are distributed in set positions combined the sample adding cavity with the washing liquid storage cavity or the elution liquid storage cavity and is used for enabling the washing liquid storage cavity or the elution liquid storage cavity to be communicated with the sample adding cavity when the touched liquid leaking mechanisms are touched by a touch structure of the pressing mechanism. The extraction system is ingenious in structural design, combines reagents with devices and can completely avoid the possibility of cross contamination.

Description

technical field [0001] The present invention relates to the technical field of molecular biology, more specifically, to the field of genetic material extraction devices, in particular to an extraction method and a corresponding system for extracting free nucleic acid in plasma by a tubular free nucleic acid separation method. Background technique [0002] Cell-free DNA, also known as circulating nucleic acid, refers to the endogenous DNA that is free in the extracellular space in circulating blood and exists in the form of single-stranded or double-stranded DNA. Most of the free DNA is double-stranded in the form of nucleoprotein strand DNA. Free DNA fragments are small, about 150-200bp in length. [0003] Cell-free DNA in blood has important potential value in the early diagnosis, prognosis and monitoring of diseases, and its specific medical applications generally include the following aspects: [0004] 1. For tumor-related analysis and medication guidance; [0005] 2. ...

AI Technical Summary

Problems solved by technology

The nucleic acid separation operation is performed through various reagent consumables. During the operation, the sample tube needs to be opened repeatedly to add different reagents, and there is a great possibility of cross-contamination.

Furthermore, the existing operation process usually takes a long time, at least about 2 hours

[0009] Moreover, some existing patents, such as patent CN 105296327A, can only be used by connecting a pump to its structure, and the pressure provided by the vacuum pump is used to transfer the liquid sample; and the washing liquid is externally placed in the external environment, which makes this method portable The performance is greatly reduced, and it can only be completed under specific experimental conditions

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