Bacterial strains for low-temperature fermentation of corn stalks and their fermentation and cultivation methods and applications
A technology of fermentation culture and fermentation medium, which is applied in the field of separation and application of straw decomposing bacteria in cold regions, and can solve the problems of low environmental temperature of straw, inconvenient transportation, difficult collection of straw, etc.
Active Publication Date: 2021-09-03
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, the utilization rate of straw in China is less than 33%, mainly because straw is difficult to collect in the field, and transportation is inconvenient, and most of them are burned or discarded.
Heilongjiang Province is a major agricultural province in China. It produces a huge amount of straw every year. Due to the low ambient temperature, low return conditions and poor promotion of these straws, many straws are burned or discarded, which not only wastes valuable crop resources, but also causes huge damage to the environment. Pollution
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Embodiment 1
[0036] Example 1 Isolation and identification of straw-decomposing bacteria in cold regions
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Abstract
The invention discloses a bacterial strain for fermenting corn stalks at low temperature, a fermentation culture method and application thereof. The invention firstly discloses an isolated bacillus pumilus strain, and its microbial preservation number is: CGMCC No.15177. The invention optimizes the relevant parameters of the fermentation preparation method of the isolated bacillus pumilus strain. The bacillus pumilus isolated in the present invention can grow at different temperatures, and still has vigorous growth under the low temperature condition of 4°C. The isolated bacterial strain of the present invention can efficiently ferment straw under low temperature conditions, and can be used alone or in combination with EM bacteria to ferment straw. After the fermented liquid of the bacterial strain of the present invention is mixed with the EM bacterial liquid, corn stalks are fermented at low temperature, and the straw fermented effect is significantly improved, and the fermented rate is increased by 47.4%. The bacillus pumilus strain isolated by the invention has broad application prospects in the preparation of straw decomposing agents, especially corn stalk decomposing agents.
Description
technical field [0001] The present invention relates to an isolated cold region corn stalk decomposing bacterial strain, and also relates to the application of the cold region straw decomposing bacterial strain in high-efficiency fermentation of corn stalks at low temperature, belonging to the field of separation and application of cold region straw decomposing bacteria. Background technique [0002] Crop straw is rich in nutrients such as cellulose, hemicellulose, lignin, protein and mineral elements, and is a renewable biological resource. However, the utilization rate of straw in China is less than 33%, mainly because straw is difficult to collect and transport in the field, and most of them are burned or discarded. Heilongjiang Province is a major agricultural province in China. It produces a huge amount of straw every year. Due to the low ambient temperature, low return conditions and poor promotion of these straws, many straws are burned or discarded, which not only wa...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05F11/08C12R1/07
CPCC05F11/08C12N1/20C05F17/20C12N1/205C12R2001/07Y02W30/40
Inventor 李凤兰徐媛媛徐永清冯艳忠杨秀梅袁强王丽娟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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