The invention relates to the field of display technologies, in particular to a display deice, a color film substrate and a manufacturing method of the color film substrate. The color film substrate comprises a substrate, a color pixel region and black matrixes, wherein the color pixel region comprises a plurality of pixel units; every pixel unit at least comprises a red sub-pixel, a blue sub-pixel unit and a green sub-pixel unit; the various color sub-pixel units in every pixel unit are arranged on one side of the substrate at intervals; and the back matrixes are arranged among the various color sub-pixel units. According to the display deice, the color film substrate and the manufacturing method of the color film substrate, the color pixel region is firstly formed, and then an inkjet process is used to form the black matrixes, so that the angle step of the pixel units is reduced to the largest extent, the flatness of the surfaces of the pixel units is increased and the incidence rate of faulted production lines is effectively reduced; besides, the process of manufacturing the black matrixes through an inkjet mode is adopted, so that, on the one hand, the input costs of preliminary stage production lines are greatly reduced, and on the other hand, the material utilization amount for manufacturing the back matrixes can be reduced and the material utilization rate is improved, and therefore, the production costs are reduced.