Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A method for promoting the accumulation of carotenoids and β-carotene in Dunaliella by utilizing β-ionone

A technology of carotene and ionone, applied in the field of food science, can solve the problems of limiting β-carotene production and long cycle, and achieve the effects of large-scale breeding, strong stress resistance, and increased production costs

Active Publication Date: 2021-09-21
SOUTH CHINA UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of cultivating Dunaliella is relatively long, which relatively limits the production of β-carotene. Finding substances that can promote the accumulation of β-carotene in Dunaliella has become a key technical problem in this field.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for promoting the accumulation of carotenoids and β-carotene in Dunaliella by utilizing β-ionone
  • A method for promoting the accumulation of carotenoids and β-carotene in Dunaliella by utilizing β-ionone
  • A method for promoting the accumulation of carotenoids and β-carotene in Dunaliella by utilizing β-ionone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Activation culture

[0035] First prepare Dunaliella liquid culture medium, its composition is: NaNO 3 0.420g / L, NaCl 87.690g / L, NaH 2 PO 4 2H 2 O 0.015g / L, NaHCO 3 0.840g / L, KCl 0.074g / L, MgSO 4 ·7H 2 O 1.230g / L, CaCl 2 2H 2 O 0.044g / L, Fe-EDTA solution (Na 2 EDTA 1.804g / L, FeCl 3 ·6H 2 O 0.483g / L) 0.5mL / L, A5 trace element solution (H 3 BO 3 2.860g / L, MnCl 2 4H 2 O 1.810g / L, ZnSO 4 ·7H 2 O 0.220g / L, CuSO 4 ·5H 2 O 0.079g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 (0.039g / L) 1mL / L;

[0036] Then the cells of Dunaliella pasteurii were inoculated in the above-mentioned liquid medium, the light intensity was 8000Lx, the light-dark cycle was 14h:10h (14h light, 10h no light) (light-dark alternating culture), the temperature was 26°C, and the temperature was 26°C, with 50r / min speed vibration culture, to obtain synchronous growth of algae liquid.

[0037] (2) Induction culture

[0038] When the algal cells in the algal liquid are in the logarithmic growth...

Embodiment 2

[0049] (1) activation culture (with embodiment 1)

[0050] (2) Induction culture

[0051] Same as Example 1, except that the culture was continued for 6 hours after adding β-ionone.

[0052] (3) pigment extraction (with embodiment 1)

[0053] (4) Content determination

[0054] The method for determining the content of total carotenoids and β-carotene is the same as in Example 1, and it is found that adding 2 to 20 mg / L of β-ionone to treat Dunaliella pasteurii for 6 hours, the ratio of total carotenoids and β-carotene content The control group without β-ionone treatment increased by 9.3-33.0% and 16.2-38.1% respectively (see figure 2 ).

Embodiment 3

[0056] (1) activation culture (with embodiment 1)

[0057] (2) Induction culture

[0058] When Dunaliella pasteurii cells were in stationary phase (OD 630 =1.421), add 0, 1, 2, 5, 20 and 50mg / Lβ-ionone to the algae liquid respectively, and continue culturing for 4 days.

[0059] (3) pigment extraction (with embodiment 1)

[0060] (4) Content determination

[0061] After calculation, it was found that adding 1, 2 and 5 mg / L of β-ionone to treat Dunaliella pasteurii for 4 days, the content of total carotenoids and β-carotene increased by 13.9-13.9% compared with the control group without β-ionone. 28.6% and 31.0~39.3%, while the high concentration of 50mg / L β-ionone was treated for 4 days, the total carotenoid and β-carotene content was significantly higher than that of the control group due to the decrease in the cell biomass of Dunaliella pasteurii. reduce (see image 3 ). It shows that low concentration of β-ionone can promote the accumulation of total carotenoids and β...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of food science and discloses a method for promoting Dunaliella to accumulate carotenoids and β-carotene by using β-ionone. The method: the Dunaliella cells to be induced are induced and cultured in a medium containing β-ionone, the carotenoids and the β-carotene in the carotenoids are accumulated in the algae cells, and then the pigment is extracted; The induced Dunaliella cells are Dunaliella cells in logarithmic growth phase and / or stationary phase. The invention is simple, easy to implement, low in cost, can significantly increase the output of total carotenoids and β-carotene, and Dunaliella is extremely salt-tolerant single-celled green algae with simple culture conditions and is not easy to be polluted by other microorganisms, which is beneficial to Large-scale breeding meets the standards of industrialized large-scale production.

Description

technical field [0001] The invention belongs to the technical field of food science, and in particular relates to a method for promoting Dunaliella to accumulate carotenoids and beta-carotene by using beta-ionone. Background technique [0002] Carotenoids are a general term for a class of important natural pigments, which are widely distributed in higher plants, algae, and some photosynthetic and non-photosynthetic bacteria and fungi. Carotenoids have important physiological functions for animals and humans, such as improving vision, anti-oxidation, improving immune function and preventing cardiovascular diseases. Carotenoids are used industrially as nutritional supplements, cosmetics, and food coloring and coloring agents. Among the carotenoids, β-carotene is a natural orange-yellow pigment. It is rich in β-carotene in carrots, papayas, mangoes, and green vegetables. Natural β-carotene is the precursor of vitamin A, which is the vitamin A in human body. It also has anti-o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 姜建国梁明华万林
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com