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The Identification Method and Application of Physiological Race of Potato Phytophthora Infestans

A technology of potato late blight and physiological race, which is applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of high identification technology requirements, heavy workload, cumbersome operation, etc. , Accelerate the progress of the experiment, simple and feasible operation

Active Publication Date: 2022-04-29
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the experimental process of identifying the physiological race of Potato infestans, Potato infestans is isolated and purified after sampling the disease samples. To identify the host, the detoxified tissue culture seedlings are usually cultivated in the room for 1-3 months before the leaves can be cut. , and each experiment needs to be repeated 3-5 times, and the experiment is guaranteed to be free from pollution. The existing identification technology of the physiological race of P. longer

Method used

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  • The Identification Method and Application of Physiological Race of Potato Phytophthora Infestans
  • The Identification Method and Application of Physiological Race of Potato Phytophthora Infestans
  • The Identification Method and Application of Physiological Race of Potato Phytophthora Infestans

Examples

Experimental program
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Embodiment 1

[0069] A method for identifying physiological races of Phytophthora infestans, comprising the following steps:

[0070] (a) Set up disease gardens: select representative fields with high incidence of potato late blight in seven provinces: Liaoning, Jilin, Heilongjiang, Shandong, Henan, Hebei and Shaanxi;

[0071] (b) Rapid Propagation in Hydroponics: The virus-free test-tube plantlets of 12 kinds of standard identification hosts from R0 to R11 were cut indoors and cultured on MS medium at a temperature of 22°C. The cultivation room is used for rapid propagation of cuttings, and after 10 days of growth, roots will form strong hydroponic seedlings;

[0072] (c) Hydroponic seedlings enter the diseased nursery: transplant the hydroponic seedlings of the identified host to the diseased nursery field in step (a), and transplant 10 plants for each identified host in each region;

[0073] (d) Disease investigation: during the epidemic period of potato late blight, with the incidence ...

Embodiment 2

[0079] A method for identifying physiological races of Phytophthora infestans, comprising the following steps:

[0080] (a) Set up disease nursery fields: select representative fields with high incidence of potato late blight in Jiangxi, Inner Mongolia, Sichuan, Yunnan, Guizhou, Hubei and Chongqing;

[0081] (b) Rapid Propagation in Hydroponics: The virus-free test-tube plantlets of 12 kinds of standard identification hosts from R0 to R11 were cut indoors and then cultured on MS medium at a temperature of 28°C. The cultivation room is used for rapid propagation of cuttings, and after 15 days of growth, roots will form strong hydroponic seedlings;

[0082] (c) Hydroponic seedlings enter the diseased nursery: transplant the hydroponic seedlings identifying the host to the diseased nursery field in step (a), and transplant 7 strains for each identified host in each region;

[0083] (d) Disease investigation: during the epidemic period of potato late blight, with the incidence of...

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Abstract

The invention belongs to the technical field of plant disease-resistant breeding, and in particular relates to a method for identifying physiological races of Phytophthora infestans and its application. In the present invention, the identification method of the physiological race of Phytophthora infestans comprises the following steps: (a) setting up the disease garden field; (b) cultivating the potato host material on the culture medium, and then carrying out hydroponics to form hydroponic seedlings; (c) ) Transplanting the hydroponic seedlings to the diseased field in step (a); (d) conducting disease investigation on the potatoes in step (c), recording the type of physiological race of potato late blight. The present invention selects natural disease gardens to carry out experimental research, taking into account the advantages of being scientific, effective and environmentally friendly. The whole experimental process does not require high professional skills, and the operation is simple and feasible. The second half of the experiment is moved to the field for identification, and the experimental effect is more significant. , which shortens the experimental time and improves the accuracy of the experiment.

Description

technical field [0001] The invention relates to the technical field of plant disease resistance breeding, in particular to a method for identifying physiological races of Phytophthora infestans and its application. Background technique [0002] Potato late blight is a typical airborne disease caused by Phytophthora infestans, which can spread rapidly when the temperature and humidity are suitable. The pathogen usually reproduces asexually; when two mating-type strains (A1 and A2) are present, they also reproduce sexually via oospores. With the migration of A2 mating type, many physiological races with stronger virulence appeared, which enhanced the disease resistance of potato infestation pathogen and aggravated the occurrence and prevalence of the disease. [0003] According to incomplete statistics, the physiological races of P. infestans infestans around the world are prone to change and tend to be complicated, and the probability and species of occurrence in different m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 肖春芳王甄张等宏沈艳芬高剑华张远学闫雷
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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