Aggregated white blood cell segmentation counting system and method

Abstract

The invention discloses an aggregated white blood cell segmentation counting system. The system comprises an image acquisition module for dyeing white blood cells in a blood sample, dissolving red blood cells in the blood sample by using red blood cell lysate and acquiring a white blood cell image, an image preprocessing module used for performing image background removal on the white blood cellimage and obtaining an optimal segmentation threshold by using a maximum inter-class variance method and roughly segmenting a white blood cell region, an aggregated cell determination module used forobtaining a coarse segmentation image according to the rough segmentation of the white blood cell region, setting a discriminant function of a cell area and obtaining a multi-cell aggregation region,and an aggregated cell segmentation counting module used for extracting a cytoskeleton in each aggregation region and a gray curve at the cytoskeleton by using a morphological refinement method. According to the invention, by analyzing the gray scale characteristics of various white blood cell areas under a low power microscope, an adaptive threshold function is constructed, while a white blood cell count is obtained, the number of oxyphil cells is obtained, the cells in the aggregation region are quickly and accurately divided and counted, the method is quick and simple and is easy to implement.

Description

technical field [0001] The present invention relates to a system and method for dividing and counting white blood cells, in particular to a system and method for dividing and counting aggregated white blood cells. Background technique [0002] White blood cell detection is an important part of clinical examination, and its content and quality are important indicators of human health. Automatic instrument analysis and manual microscopic examination are the main methods for leukocyte classification and counting at present, but they still have the disadvantages of bulky equipment, difficult maintenance and highly professional operation, which are not suitable for primary medical and health institutions, mobile medical and home use. The image-based portable white blood cell counter can obtain accurate diagnostic information by analyzing blood sample images at the place closest to the patient, which has time and space advantages that large-scale instruments do not have. However,...

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Problems solved by technology

[0005] Purpose of the invention: In order to overcome the deficiencies of the prior art, the present invention provides a system and method for dividing and counting aggregated white blood cells, which can solve the problem of inability to obtain accurate cell segmentation and complete separation of aggregated cells, especially highly aggregated cells, and the accuracy of counting low problem

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