Cell counting instrument and application thereof

Abstract

The invention provides a cell counting instrument and an application thereof and relates to the technical field of biological particle detection. The cell counting instrument comprises a positive pressure control unit, a negative pressure control unit, a counting module, a processing system, an effluent container and a sheath fluid container, wherein the counting module comprises a micro-fluidic chip. Due to the arrangement of a liquid inlet, a feeding hole, an effluent hole and a micro-channel on the micro-fluidic chip and the arrangement of an electric pulse signal detection area on the micro-channel, the whole liquid flow system is more compact and effective and the absolute counting of whole sample amount is realized, so that the result accuracy and consistency of cell counting are better than those of the present method and the problems of low integration level of the instrument, small detected sample amount and inaccurate result caused by cell layering and suspension in the priorart can be relieved. The counting module is designed in a detachable form, so that the flexibility of the cell counting instrument system is promoted and the cell counting instrument can meet different detection requirements of users. The invention also provides the application of the cell counting instrument.

Description

technical field [0001] The invention relates to the technical field of biological particle detection, in particular to a cell counter and its application. Background technique [0002] In a large number of cell biology research experiments, it is necessary to perform concentration detection (quantitative counting) on ​​cells or other biological particles. The concentration (or quantity) of cell suspension is not only a monitoring parameter for cell culture, but also a necessary parameter in many experimental projects. Completing the experiment is a very basic but important process condition. Existing counting methods mainly include manual counting methods using cell counting plates, automatic counting instruments based on image analysis technology, and automatic counting instruments using the resistance method (Coulter's principle). [0003] Among them, the manual counting method is the most common. The experimenter injects the suspended cell sample into the counting pool o...

AI Technical Summary

Problems solved by technology

[0007] The first purpose of the present invention is to provide a cell counter. By adopting microfluidic chip technology, the entire liquid flow system is more compact and effective, and the absolute counting of the entire sample volume is realized, thereby making the result of cell counting more accurate. The consistency and consistency are better than the existing devices, and the problems of inaccurate results caused by the low integration of the instrument in the prior art and the small amount of detection samples and the layered suspension of cells are improved.

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