The invention relates to a single well-injection and water-suction tracer experiment simulation analysis system. The single well-injection and water-suction tracer experiment simulation analysis system comprises a well body, a geological water-containing layer simulation module, an overflow device, a distilled water box, a tracer box, a sucked liquid storage box, and a background data processing and simulation analysis terminal, wherein a water containing area and a flow stabilizing area, which are communicated with each other, are arranged in the geological water-containing layer simulation module, and are respectively filled with a water-saturated medium and a flow stabilizing medium; the well body and the overflow device are respectively communicated with the water containing area and the flow stabilizing area; a liquid inlet pipe and a liquid outlet pipe are arranged on the well body, and the distilled water box and the tracer box are respectively communicated with the liquid inlet pipe through pipelines; one end, away from the well body, of the liquid outlet pipe is communicated with the sucked liquid storage box; a plurality of layers of liquid concentration sensors are uniformly distributed in the water containing area, and a plurality of liquid concentration sensors are arranged on each layer; the liquid concentration sensors are electrically connected with the background data processing and simulation analysis terminal. The single well-injection and water-suction tracer experiment simulation analysis system has the advantages that the structure is simple, the operation is convenient, and the testing of single well-refilling and water suction tracer experiment can be performed at high accuracy and high frequency.