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Tissue culture method of iris florentina

A technique for tissue culture and iris vetiver, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of unstable extraction yield, uneven quality, and long reproduction cycle of ornamental condensed body of iris vetiver To achieve high industrial promotion value, maintain parental traits, and reduce production costs

Inactive Publication Date: 2018-12-18
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the stimulation of the high price of the iris condensate and the lucrative profit margins, various domestic companies have also carried out the fermentation and extraction of the condensate and the planting of the iris vetiver. Long, low reproductive rate, serious accumulation of diseases and insect pests and other factors, the current domestic planting scale is developing slowly, and the supply of domestic raw materials is far from enough to meet the market demand for raw materials
At the same time, there are many strains among Iris vetiver species, and the quality is uneven, which makes Iris vetiver unstable both in terms of its ornamental value and the extraction yield of condensed fragrance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Select the whole plant of vetiver iris of high-quality strain, plant it in the laboratory with peat soil sterilized by high pressure steam, irrigate the roots with 800 times liquid of 75% carbendazim powder every 7 days, take out the plant after 50 days, and remove it. Produce fibrous roots and aboveground stems and leaves, etc., and leave bulbs with buds as explants.

[0019] 2. Use a toothbrush to remove the soil on the surface of the bulbs with buds, then rinse with detergent solution for 10 minutes, rinse 4 times and then rinse with tap water for 80 minutes.

[0020] 3. Transfer the treated explants to the ultra-clean workbench, soak in 3% hydrogen peroxide for 2 hours, rinse with sterile water twice, sterilize with 0.1% mercuric acid + 2 drops of Tween-80 solution for 4 minutes, and rinse with sterile water Rinse 3 times, sterilize with 0.2% sodium hypochlorite solution for 25 minutes, rinse with sterile water 3 times, sterilize with 0.1% mercuric solution for 1...

Embodiment 2

[0025] 1. Select the whole plant of vetiver iris of high-quality strain, plant it in the laboratory with sterilized peat soil, irrigate the roots with 800 times liquid of 75% carbendazim powder every 7 days, and then take out the plant, remove the fibrous roots and the ground Stems and leaves, etc., leave bulbs with buds as explants.

[0026] 2. Brush the bulbs with buds to remove the surface soil with a toothbrush, then rinse with detergent solution for 20 minutes, rinse 4 times and then rinse with tap water for 60 minutes.

[0027] 3. Transfer the treated explants to the ultra-clean workbench, soak in 3% hydrogen peroxide for 2 hours, rinse with sterile water twice, sterilize with 0.1% mercury liter + 2 drops of Tween-80 solution for 5 minutes, and rinse with sterile water Rinse 4 times, sterilize with 0.2% sodium hypochlorite solution for 20in, rinse with sterile water 3 times, sterilize with 0.1% mercuric solution for 10min, rinse with sterile water 8 times.

[0028] 3. U...

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PUM

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Abstract

The invention discloses a tissue culture method of iris florentina. The tissue culture method comprises the following steps: (1) disinfecting an explant; (2) inducing clustered shoots; (3) performingthe multiplication culture; and (4) rooting. By adopting the tissue culture method of provided by the invention, the clustered shoot induction rate, multiplication coefficient and rooting rate of theiris florentina can be significantly improved. The tissue culture efficiency is high, the cost is low, and the mass application value can be achieved.

Description

technical field [0001] The invention relates to the field of tissue culture of Chinese medicinal materials, in particular to a method for tissue culture of iris vetiver. Background technique [0002] Iris pallida Lam. is a perennial herb of the family Iridaceae. Its flowers are large and showy. It is a common garden ornamental flower and is the national flower of France. Many families in my country also use it as a garden ornamental plant. Vetiver iris also has a relatively delicate fragrance, and is also used by some enterprises and individuals to enter the market as cut flowers. At the same time, the rhizome of vetiver orris is the most widely used raw material for the extraction of aromatic oils of iris. After 2-5 years of storage, it extracts a kind of precious spices like violet series - iris extract, essential oil, gelatin, etc., which are called Iris cream is also relatively condensed, and is widely used in cosmetics or pharmaceutical flavoring agents and daily chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓国宾邓清杨燕刘祥义曾祥飞赵露琴张媛曾琼蒋元省
Owner SOUTHWEST FORESTRY UNIVERSITY
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