Method for performing induced mutation on griseofulvin strain through diethyl sulfate and ultraviolet rays

Abstract

The invention discloses a method for performing induced mutation on griseofulvin strain through diethyl sulfate and ultraviolet rays. The method comprises the following steps: firstly, crushing selected bacterial colony through glass balls, preparing spore suspension, sucking 1ml of single spore suspension, diluting to obtain a contrast, and adding filtrate to double discs in which a diethyl sulfate containing culture medium is prepared in advance; uniformly irradiating for 30mn through the ultraviolet rays, covering with a black cloth, diluting with sterile water, uniformly applying onto a flat vessel to obtain a single bacterial colony, transferring the single bacterial colony into a constant-temperature box for culturing, selecting a mitogenetic single bacterial colony 8 days after thebacterial colony grows, and transferring the mitogenetic single bacterial colony into a bevel for culturing for 8 days; transferring the manure bacterial colony into a shake flask for primarily screening, selecting 10 bevels of which the potency unit is 3% more than the beginning bacterial colony, transferring the bevel, culturing until the bacterial colony is manure, and screening again through the shake flask; selecting 5 bevels with relatively high unit after screening again, burying the bevels with corresponding numbers into sandy soil, and storing. With the adoption of the method, the fermenting unit can be further increased; and the strain can remain outstanding properties.

Description

technical field [0001] The invention belongs to the technical field of mutagenesis methods for griseofulvin strains, and in particular relates to a method for mutagenizing griseofulvin strains by diethyl sulfate and ultraviolet rays. Background technique [0002] Griseofulvin is an antibiotic raw material drug. Its Chinese aliases are griseofulvin and microcrystalline griseofulvin. The finished product of griseofulvin is white or off-white fine powder. Superficial fungi such as fungus have good antibacterial effect. Griseofulvin is suitable for the treatment of various ringworm diseases, including tinea capitis, tinea barbae, tinea corporis, jock itch, tinea pedis and onychomycosis. In the prior art, the method for preparing griseofulvin in large quantities generally uses the griseofulvin fermentation liquid as a raw material. After a series of preparation steps, the griseofulvin recrystallization is obtained through concentration and crystallization, and finally the griseof...

AI Technical Summary

Problems solved by technology

In the prior art, the griseofulvin strain in the griseofulvin fermentation liquid is not good enough, and the strain problem will directly affect the quality and output of the finished griseofulvin raw material drug, so it is necessary to improve the preparation of griseofulvin method, to improve the yield, we must start from the source, that is, we need to adopt high-quality griseofulvin strains to prepare griseofulvin fermentation broth