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Liquid medium, preparation method for phlebopus portentosus industrial liquid stains and application of liquid medium

A technology of liquid culture medium and dark brown reticulum, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems that factory culture cannot be realized, and achieve stable mycelium growth, which is easy to control and easy to produce. collection, low cost effect

Inactive Publication Date: 2018-12-28
周茂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that industrialized cultivation cannot be realized in the prior art, the present invention proposes a simple and efficient Dictyostelium boletus liquid culture medium capable of realizing industrialized cultivation and the preparation of Dictyostelium obsatus industrialized culture liquid spawn method

Method used

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  • Liquid medium, preparation method for phlebopus portentosus industrial liquid stains and application of liquid medium
  • Liquid medium, preparation method for phlebopus portentosus industrial liquid stains and application of liquid medium
  • Liquid medium, preparation method for phlebopus portentosus industrial liquid stains and application of liquid medium

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Experimental program
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Effect test

Embodiment 1

[0019] A kind of liquid culture medium, comprises following composition and each composition content is as follows:

[0020]

[0021] A kind of method that utilizes described liquid culture medium to prepare the factory liquid bacterial classification of Dictyostelium bursa, comprises the following steps:

[0022] Step A: using the mother seed culture medium to culture the fruiting body tissue pieces of Dictyostelium pheasatum to prepare the mother seed. Include the following steps:

[0023] Step a1: Prepare stock culture medium.

[0024] Add the following ingredients to the container:

[0025]

[0026] After the addition, boil until the agar is completely melted, adjust the pH to 5.8 to obtain the mother seed medium, add it quantitatively to the test tube, sterilize at 115°C for 30 minutes, place it on an inclined plane, and cool it down for later use.

[0027] Step a2: Inoculate the isolated tissue pieces of the fruiting body of Dictyostelium bruceae into the cultur...

Embodiment 2

[0033] A kind of liquid culture medium, comprises following composition and each composition content is as follows:

[0034]

[0035] A kind of method that utilizes described Dictyostelium phelicus liquid culture medium to prepare the industrialized liquid strain of Dictyostelium pheasatus, comprises the following steps:

[0036] Step A: Preparing the mother seed by cultivating the preserved mycelium block with the mother seed medium, including the following steps:

[0037] Step a1: Prepare stock culture medium. Add the following ingredients to the container in the following quantities:

[0038]

[0039] After adding, boil until the agar is completely melted, adjust the pH to 6.2 to obtain the mother seed medium, add it quantitatively to the test tube, sterilize at 121°C for 20 minutes, put it on an inclined plane, and use it after cooling.

[0040] Step a2: Inoculate the preserved mycelium block on the mother seed medium, and culture at a constant temperature of 29° C...

Embodiment 3

[0045] A kind of liquid culture medium, comprises following composition and each composition content is as follows:

[0046]

[0047] A kind of method that utilizes described Dictyostelium phelicus liquid culture medium to prepare the industrialized liquid strain of Dictyostelium pheasatus, comprises the following steps:

[0048] Step A: Preparing the mother seed by cultivating the preserved mycelium block with the mother seed medium, including the following steps:

[0049] Step a1: Prepare stock culture medium. Add the following ingredients to the container in the following quantities:

[0050]

[0051]

[0052] After the addition, boil until the agar is completely melted, adjust the pH to 6.8 to obtain the mother seed medium, add it quantitatively to the test tube, sterilize at 118°C for 25 minutes, put it on an inclined plane, and use it after cooling.

[0053] Step a2: Inoculate the isolated tissue pieces of the fruiting bodies of Dictyostelium obliquus into the...

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Abstract

The invention provides a phlebopus portentosus medium and a method for quickly culturing the phlebopus portentosus industrial liquid stains by the adoption of the medium. A mother strain medium and aliquid strain medium are prepared from molasses, sucrose, KH2PO3, MgSO4, yeast extract, agar and water and the like according to a certain ratio, mother strains are prepared by using boletes fruitingbody tissue blocks or preserved hyphae, and fermentation culture is carried out by a liquid culture method. The invention also provides application of the phlebopus portentosus liquid strain medium inphlebopus portentosus liquid strain culture and / or fermentation tank fermentation culture. The phlebopus portentosus liquid strain medium and the method for quickly culturing the phlebopus portentosus industrial liquid strains by adopting the medium is simple, convenient and efficient, can overcome the deficiencies of the prior art, and realize the industrial large-scale cultivation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a liquid culture medium and its application, and a method for cultivating a liquid strain of Dictyostelium pheasatum. Background technique [0002] Phelebopus portentosus, also known as black boletus, is a rare edible fungus, which is delicious and nutritious, and has a broad market prospect as an edible fungus. However, the number of such bacteria that grow naturally is very limited. In order to meet the needs of consumers for Dictyostelium bruceae, it is necessary to develop an artificial cultivation method. With the development of research on the cultivation and planting technology of Dictyostelium bruceae in recent years, it has been possible to carry out medium-scale cultivation and planting with bag planting and bottle planting methods at present, but the prior art still fails to overcome technical difficulties and realize Large-scale planting. [0003] The stable culture of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 周茂唐凯唐林李庆
Owner 周茂
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