Research method for analyzing Klotho interaction protein in non-small cell lung cancer

A non-small cell lung cancer, protein technology, applied in the field of biological applications, can solve problems such as cognitive limitations, and achieve the effect of improving the distribution level of Klotho

Active Publication Date: 2018-12-28
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been many studies on the tumor suppressor effect of Klotho, the research mainly focuses on its regulation of downstream signals. The understanding of the regulation of Klotho itself and the interaction between Klotho's mutual binding proteins is still very limited.

Method used

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  • Research method for analyzing Klotho interaction protein in non-small cell lung cancer
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  • Research method for analyzing Klotho interaction protein in non-small cell lung cancer

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Embodiment Construction

[0049] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0050] A research method for analyzing Klotho-interacting proteins in non-small cell lung cancer, comprising the following steps:

[0051] 1. Screening of Klotho-interacting proteins

[0052] 1.1 Klotho lentiviral expression vector Lenti-FLAG-Klotho was constructed and infected with lung cancer cells

[0053] The pUltra lentiviral vector was purchased from Addgene. Human KlothocDNA was cloned into the XbaI-BamHI site, downstream of the 2a peptide coding sequ...

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Abstract

The invention discloses a research method for analyzing a Klotho interaction protein in non-small cell lung cancer which comprises the three steps, a screening of Klotho interaction protein, a verification of Rab8 and Klotho gene binds to one another, and the mechanism analysis of Rab8 and Klotho interaction. The present invention firstly finds that the presence of Rab8 and Klotho in non-small cell lung cancer cells binds to one another, and a co-location exist in the subcellular, Rab8 binds to Klotho through a protein synthesis pathway and promotes a transport thereof to the membrane surfacein the form of vesicles, so that the level of the surface of the membrane is increased.

Description

technical field [0001] The invention relates to a protein, specifically a research method for analyzing Klotho interaction protein in non-small cell lung cancer, and belongs to the field of biological application. Background technique [0002] Lung cancer ranks first among malignant tumors in terms of morbidity and mortality worldwide. Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer, and its 5-year survival rate is about 10%, and its incidence is still rising. Therefore, it is of great significance to explore the underlying mechanism of NSCLC and develop safer and more effective treatments. Lung cancer is an aging-related disease. The median age of lung cancer diagnosis is 70 years old, and the peak incidence of lung cancer is in the age group of 75-79 years old. With the aging process, genomic defects, epigenetic gene silencing, oxidative stress, defective immune surveillance, telomere abnormalities, chronic inflammation, and changes in the ti...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12Q1/686
CPCC12Q1/686G01N33/6845C12Q2531/113C12Q2525/207
Inventor 吴剑卿陈波赵卫红
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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