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Construction method of human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and detection platform thereof

A technology of mesenchymal stem cells and construction methods, applied in the field of construction methods and detection platforms of human umbilical cord mesenchymal stem cell seed banks, can solve the impact of mesenchymal stem cell safety, incomplete information construction, and insufficient coverage of cell detection, etc. problems, to achieve the effect of shortening the exposure time, activating self-healing ability, and reducing the risk of contamination

Inactive Publication Date: 2019-01-11
EASTERN UNION STEM CELL & GENE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still shortcomings in the existing stem cell bank building methods, such as: many separation steps, high risk of contamination, introduction of exogenous animal protein, incomplete testing items, imperfect information construction, etc.
[0012] The above method has some shortcomings and deficiencies: the isolation and culture steps are cumbersome, and the operating consumables are time-consuming; the coverage of cell detection is insufficient; the exogenous protein contained in the reagent affects the safety of mesenchymal stem cells; the information construction is not perfect

Method used

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  • Construction method of human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and detection platform thereof
  • Construction method of human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and detection platform thereof
  • Construction method of human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and detection platform thereof

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Embodiment 1

[0066] combine figure 1 , this embodiment describes in detail the construction method of the human umbilical cord mesenchymal stem cell seed bank of the present invention, as figure 1 As shown, it includes the following steps:

[0067] S11: Take the human umbilical cord for safety testing;

[0068] In one embodiment, step S11 specifically includes:

[0069] (1) Collection: Aseptically collect human umbilical cord, disinfect, extract a small amount of umbilical cord venous blood for pathogenic detection, and put the umbilical cord into a sterile disposable umbilical cord collection bottle;

[0070] (2) Transportation: put the human umbilical cord bottle into a special cold chain storage box, without X-ray irradiation and away from the radiation source, through the real-time temperature monitoring equipment (keep the temperature of the storage box at 2-8 °C all the time) ), the transport cold chain of the location positioning system, and select the best transport route to del...

Embodiment 2

[0104] This embodiment describes the detection platform of the present invention in detail, which includes: umbilical cord donor personal information unit, collection and reception record unit, specimen inspection record unit, test result report unit, separation record unit, reagent batch number unit, separation and handover confirmation unit , a cultivation record unit, a frozen storage storage information unit, and a record unit for each process operator; wherein, the above-mentioned units correspond one-to-one. The information in the process of constructing the human umbilical cord mesenchymal stem cell seed bank in the above-mentioned examples was entered into the detection platform in time, and each link was organically connected in series to establish a large database for quick retrieval of operation records and cell information files.

[0105] The detection platform of this embodiment has added information construction and management methods that match the construction p...

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Abstract

The invention discloses a construction method of a human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and a detection platform thereof. The method comprises the steps of carrying out safetydetection on a human umbilical cord; disinfecting and washing by using a washing liquid; cutting a preset length of root of the human umbilical cord and washing by using the washing liquid; cutting into small blocks of preset size, adding a culture medium, uniformly mixing, centrifuging, discarding the supernatant, centrifuging again and removing the residual washing liquid; uniformly mixing thecentrifuged and precipitated tissue blocks with a culture medium and transferring to an incubator to culture; carrying out amplification; and carrying out cryopreservation. The platform comprises: anumbilical cord donor personal information unit, a collecting, receiving and recording unit, a specimen submission recording unit, a detection result reporting unit, a separation recording unit, a reagent batch number unit, a separation handover confirming unit, a culture recording unit, a cryopreservation and storage information unit and a process operator recording unit. The construction method of the human umbilical cord mesenchymal stem cell (UCMSCS) seed bank and the detection platform thereof, which are disclosed by the invention, have the advantages of simplifying the separation step, reducing the energy consumption and consumables and increasing the cell collecting efficiency.

Description

technical field [0001] The invention relates to the technical field of stem cell banks, in particular to a method for constructing a human umbilical cord mesenchymal stem cell seed bank and a detection platform. Background technique [0002] Umbilical cord mesenchymal stem cells (UCMSCs) refer to a kind of mesenchymal stem cells existing in umbilical cord Wharton's jelly (Wharton's jelly) and perivascular tissue, which have high differentiation potential and can Differentiate in multiple directions. The mesenchymal stem cells derived from the umbilical cord are easy to obtain because of their convenience, no moral and ethical disputes, a large number of cells that can be obtained, strong proliferative ability, strong immune regulation, and the total amount of secreted cell growth factors is also very high, which is convenient for expansion and passage , has broad clinical application prospects in tissue engineering such as bone, cartilage, muscle, tendon, ligament, nerve, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0665C12N2509/00
Inventor 徐志国任振辉刘超钮移坤
Owner EASTERN UNION STEM CELL & GENE ENG
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