Self-adaptive fluorescence immunochromatography quantitative detection feature extraction method

Abstract

The invention discloses a self-adaptive fluorescence immunoassay quantitative detection feature extraction method, and belongs to the technical field of biology, medical engineering and computer signal processing. According to the method, fluorescence signals are obtained on the basis of a fluorescence immunoassay quantitative detection and measurement technology; and on the basis of carrying outdata processing such as filtration and the like on the fluorescence signals, the data processing carried out on the fluorescence signals comprises signal peak value recognition, boundary determination, fluorescence line fitting, base line fitting and feature value determination. In order to ensure the self-adaptability and correctness of the measurement and solve the problem that current fluorescence immunoassay quantitative detection has the phenomenon that fluorescence signal measured values are saturated and cannot be calculated, the invention discloses the self-adaptive fluorescence immunoassay quantitative detection feature extraction method to ensure the correct measurement.

Description

technical field [0001] The invention belongs to the technical fields of biology, medical engineering and computer signal processing. Specifically, it relates to an adaptive feature extraction method for fluorescent immunochromatography quantitative detection, in particular, a method for adaptively dividing data regions using currently measured fluorescence signals to perform accurate feature data extraction. Background technique [0002] Fluorescence Immunoassay (FIA) is the earliest established immune labeling technique, created by Coons et al. in the early 1940s. The basic principle is to combine the sensitive measurability of fluorescence with the highly specific reaction of antigen and antibody, and use fluorescent substances as markers. Under the action of excitation light of a specific wavelength, fluorescent substances can absorb light energy to enter an excited state, release the previously absorbed light energy in the form of electromagnetic radiation, and generate...

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Problems solved by technology

In this case, some directly take the area in order to reduce the effect, and some use the method of reducing the excitation light for multiple excitations. These methods have many problems. First, the measurement result is still affected by directly taking the area itself. When the saturation When the phenomenon is serious, the area cannot complete the weakening effect at all; and by reducing the excitation intensity of the excitation light, on the one hand, multiple excitations greatly prolong the measurement time, on the other hand, multiple excitations will increase the uncertainty of the measurement process, and more Importantly there is more risk of fluorescence quenching during multiple excitations

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