High-quality and high-yield medicinal bombyx batryticatus breeding method

A breeding method and high-yield technology, applied in the direction of animal husbandry, etc., can solve the problems of lack of more detailed research, affecting the production of high-quality silkworms, and uneven quality of silkworms, so as to ensure the production of high-quality silkworms and benefit silkworms Effects of growth, ease of standardization

Pending Publication Date: 2019-01-18
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Beauveria bassiana has a strong impact on silkworms, there is no relevant literature report or more detailed research on whether the breeding method of silk cocoons is also applicable...

Method used

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  • High-quality and high-yield medicinal bombyx batryticatus breeding method
  • High-quality and high-yield medicinal bombyx batryticatus breeding method
  • High-quality and high-yield medicinal bombyx batryticatus breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The method of feeding before the silkworm inoculation

[0031] 1. Experimental materials

[0032] The 5th instar larvae of the silkworm Bombyx mori Linnaeus, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0033] 2. Experimental design

[0034] Beauveria bassiana stock solution was prepared with sterile water containing 0.05% Tween 80 (23-28°C), the spore concentration was measured by a hemocytometer, and the stock solution of Beauveria bassiana was diluted to 1×10 8 Pieces / mm2. Set 4 feeding times, 0 feeding before inoculation, 1 feeding, 2 feeding, 3 feeding, 3 repetitions in each group, a total of 12 treatment areas. The feeding temperature is 26±2℃, the humidity is 70±5%RH, the light / dark (10:14) environment, and the amount of mulberry is sufficient to satisfy the appetite.

[0035] 3. Results and Analysis

[0036] 3.1 Effects of feeding before inoculation on silkworm growth indicators

[0037] Feeding times increased, the dead time was prolonged,...

Embodiment 2

[0052] Embodiment 2 silkworm inoculation method

[0053] 1. Experimental materials

[0054] The 5th instar larvae of the silkworm Bombyx mori Linnaeus, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0055] 2. Experimental design

[0056] 2.1 Screening of vaccination methods

[0057] Healthy fifth-instar silkworm larvae were selected for inoculation. Beauveria bassiana stock solution was prepared with sterile water containing 0.05% Tween 80 (23-28°C), the spore concentration was measured by a hemocytometer, and the stock solution of Beauveria bassiana was diluted to 1×10 8 Pieces / mm2. The blank group and the experimental group were set up, the blank group was not inoculated, and the experimental group adopted 3 inoculation methods, mode 1 (infiltration), mode 2 (spray), and mode 3 (feeding), each group was repeated 3 times, a total of 12 treatment areas . After inoculation, the ambient temperature is 24-26 ℃, and the humidity is 90±5% RH. The breeding temperature was 26...

Embodiment 3

[0084] Embodiment 3 silkworm inoculation time selection method

[0085] 1. Experimental materials

[0086] The 5th instar larvae of the silkworm Bombyx mori Linnaeus, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0087] 2. Experimental design

[0088] According to the results of Example 1, because the time used for feeding silkworms once is different, it is not easy to control in actual production, so the experiment was carried out without feeding before inoculation.

[0089] 2.1 Preliminary study on vaccination time

[0090] Beauveria bassiana stock solution was prepared with sterile water containing 0.05% Tween 80 (23-28°C), the spore concentration was measured by a hemocytometer, and the stock solution of Beauveria bassiana was diluted to 1×10 8 Pieces / mm2. Beauveria bassiana working solution was inoculated on the 0th, 2nd, 4th and 6th hours of silkworm picking, with 3 replicates in each group, for a total of 12 treatment areas. The feeding temperature is 26±2℃, the ...

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Abstract

The invention provides a high-quality and high-yield medicinal bombyx batryticatus breeding method. The method comprises the following steps of a, culture in an early stage; b, inoculation; c, expansion; d, feeding before being stiff; e, harvesting processing. The high-quality and high-yield medicinal bombyx batryticatus breeding method adopts a spray inoculation method, does not affect silkworm inoculation, provides convenience for standardize operation, and is suitable for industrialized large-scale production; for different newly exuviated larva of the silkworm in the production, differentfeeding are set before inoculation, which not only effectively guarantees the mortality rate of the silkworm, but also facilitates the growth of the silkworm, and the yield is increased; the method ensures the high quality silkworm production to the largest extent by improving the yield through reasonable density configuration; in different growth stages of the silkworm, through the feeding treatment and the addition of medicament, the growth of the silkworm and the inoculation effect of beauveria bassiana can be considered, and the quality of bombyx batryticatus medicinal materials can be ensured while the yield is increased.

Description

technical field [0001] The invention relates to a high-quality and high-yield medicinal silkworm breeding method. Background technique [0002] Bombyx mori is derived from the dried body of Beauveria assiana (Bals.) Vuillant of the 4th to 5th instar larvae of silkworm Bombyx mori Linnaeus. Fibrinolysis, anti-cancer, lipid-lowering, beauty and other pharmacological effects. With the in-depth research on the pharmacological components and functions of the silkworm, the application will become more and more extensive. The quality of the silkworm will greatly affect its research and development, so the research on the breeding method of high-quality and high-yield silkworm is very important. [0003] Yang Qiong, et al. Research on the artificial culture of medicinal white silkworm, Guangdong Sericulture 44(4): 34-38 discloses the artificial culture method of white silkworm, screening of strains, optimal inoculation dose, suitable silkworm varieties, etc. study, when its inocu...

Claims

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Application Information

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IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 李敏陈辉陈蓉敬勇
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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