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A method for extracting biological cells on a physical evidence object

A technology of biological cells and extraction methods, which is applied in the field of extraction of biological cells on physical evidence objects, can solve the problems of difficult extraction, low degree of exposure, and high concealment, and achieve the effect of improving the detection rate

Active Publication Date: 2021-03-19
SUZHOU XIAOSONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the small size of biological cell particles, especially the biological cells that have been shelved for a long time and have been fully dried, the contact between the cell body and the surface of the evidence object is high, and the contact attraction and collagen adhesion between the cell and the object surface can be much greater than that of the wipe. In addition, the cells still have the characteristics of high concealment, low exposure, and difficult extraction. It is not easy to fully collect the biological cells left on the criminal evidence by using traditional extraction methods.

Method used

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  • A method for extracting biological cells on a physical evidence object
  • A method for extracting biological cells on a physical evidence object
  • A method for extracting biological cells on a physical evidence object

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the extraction method of biological cell on stone, comprises the steps:

[0037] (1) Use the bioluminescent agent of Suzhou Xiaosong Technology Development Co., Ltd. to visualize the biological traces on the stone. The range of biological traces is less than or equal to 0.5 square centimeters.

[0038] (2) Use the head of the swab soaked in distilled water with a head width of 0.2 mm to 2 mm to rotate and wipe the biological trace position on the stone; the pressure is rotated and the pressure is controlled from heavy to light. The swab is wiped, and the rotation direction of the previous swab is opposite to that of the subsequent swab.

[0039] (3) Wait for step (2) for 5 minutes after the extraction, use fiber diameters of 0.01 mm to 0.05 mm, elastic recovery rate ≥ 80% when elongated 3%, dry fibers with a dielectric constant ≤ 4 at 106 Hz The swab is rotated and wiped again at the biological trace position; the pressure is rotated and the pressure is c...

Embodiment 2

[0042] Embodiment 2: the extraction method of biological cell on the stone, comprises the steps:

[0043] (1) Use the bioluminescent agent of Suzhou Xiaosong Technology Development Co., Ltd. to visualize the biological traces on the stone. The range of biological traces is less than or equal to 0.5 square centimeters.

[0044] (2) Rotate and wipe the biological trace position on the stone with the head of a swab soaked in distilled water with a head width of 0.2-2mm; rotate the pressure and control the pressure from heavy to light. The swab is wiped, and the rotation direction of the previous swab is opposite to that of the subsequent swab.

[0045] (3) Wait for 5 minutes after the extraction in step (2), and wipe with a dry fiber with a fiber diameter of 0.01 mm to 0.05 mm, an elastic recovery rate of ≥80% when elongated at 3%, and a dielectric constant of ≤4 at 106 Hz The swab is rotated and wiped again at the biological trace position; the pressure is rotated and the press...

Embodiment 3

[0060] Embodiment 3: the extraction method of biological cell on brick, comprises the steps:

[0061] (1) The bioluminescent agent of Suzhou Xiaosong Technology Development Co., Ltd. is used to visualize the biological traces on the bricks, and the range of biological traces is greater than 0.5 square centimeters.

[0062] (2) Rotate and wipe the body of the swab soaked in distilled water with a body length of 2-20 mm at the biological trace position on the brick; each swab only rotates once, and three swabs are used for the biological trace position Swabs are made with the previous swab rotating in the opposite direction to the latter swab.

[0063] (3) Wait for 5 minutes after the extraction in step (2), and use a dry fiber wipe with a fiber diameter of 0.01mm-0.05mm, an elastic recovery rate of ≥80% when elongated at 3%, and a dielectric constant of ≤4 at 106 Hz The swab is rotated and wiped again at the biological trace position; each swab only rotates once, and the biolo...

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Abstract

The invention relates to a method for extracting biological cells on physical evidence objects. The method comprises the following steps: (1) wiping a biological trace position of a physical evidenceobject by a wet swab; (2) waiting for 5 to 10 minutes after the extraction in the step (1), wiping the biological trace position of the physical evidence object by a dry fiber swab; and (3) wiping thebiological trace position of the physical evidence object by a swab adhered with biological cell extracting solution. The method is capable of sufficiently and effectively carrying out cell extraction through multi-step extraction, so as to provide the guarantee for subsequent DNA identification and improve the detection rate.

Description

technical field [0001] The invention relates to a method for extracting biological cells on a physical evidence object. Background technique [0002] In the field of criminal evidence investigation, it is necessary to discover and acquire criminal evidence left by suspects, including fingerprint evidence and trace evidence containing DNA characteristics, so as to build a criminal evidence chain and lay a legal evidence foundation for punishing crimes. [0003] At present, Xiaosong Technology has been able to quickly and accurately lock the location of biological traces through laser irradiation fluorescence display technology. However, after the location of the biological trace is locked, the biological cells at the location still need to be fully and effectively extracted in order to provide a strong guarantee for subsequent DNA identification. However, the phenomenon of "visible position but no DNA detection" often occurs at present, mainly because too little cell tissue ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00
CPCC12N5/00C12N2509/10
Inventor 胡晓松
Owner SUZHOU XIAOSONG TECH DEV
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