A method for separating and preparing EGCG from tea leaves

A technology of tea and epigallocatechin, which is applied in the field of separation and preparation of EGCG, can solve the problems of high cost of EGCG, unsafe operation, high cost of raw materials, etc., and achieve the effects of reducing the concentration process, simple production process and low production cost

Active Publication Date: 2016-08-24
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, the above method has the following problems: (1) using oolong tea or even green tea polyphenols as raw materials for manufacturing, the processing of these tea leaves and the production process of tea polyphenols lead to significantly higher raw material costs, lower production rates, and higher overall cost of forming EGCG; (2) A large number of organic reagents such as metal salts and chloroform have been used in the process, which has brought a series of safety problems such as environmental pollution and unsafe operation, and EGCG products have potential safety hazards such as excessive solvent residues, which are not conducive to food production. Promotion with pharmaceutical and other industries

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Put 30kg of tea products that are directly greened and dried without traditional processing into an extraction tank with a volume of 0.5T, spray evenly 90L of pure water at a temperature of 40°C from the top of the extraction tank, and soak for 40 minutes. Then spray 90L of pure water at 60°C evenly at a flow rate of 30L / h, and start discharging at a flow rate of 30L / h at the same time. On the AMBERLITE XAD4 column, the column volume is 60L. After all the materials are loaded on the column, elute with 180L pure water, and then analyze with 120L 20% ethanol aqueous solution at a flow rate of 60L / h. After loading the column, elute the PA column with 60L of 30% ethanol aqueous solution, and finally use 120L of 80% ethanol for analysis, collect the 80% ethanol analysis solution, concentrate the analysis solution to a solid content of 30-35%, and place the concentrated solution at 5°C crystallization. After the crystals were washed by suction filtration, they were vacuum-d...

Embodiment 2

[0040]Put 30kg of picked tea leaves into an extraction tank with a volume of 0.5T, spray evenly 75L of pure water at a temperature of 70°C from the top of the extraction tank, and soak for 30min. Then spray 100L of pure water at 85°C evenly at a flow rate of 25L / h, and start discharging at a flow rate of 25L / h at the same time (the temperature of the feed liquid is cooled to below 25°C through a cooling device), while discharging at a rate of 25L / h The flow rate is on the AMBERLITE XAD4 column, and the column volume is 20L. After all the materials are loaded on the column, elute with 80L of pure water, and then analyze with 40L of 25% ethanol aqueous solution at a flow rate of 20L / h. . After loading on the column, elute with 20L of 25% ethanol aqueous solution, and finally analyze with 40L of 85% ethanol, collect the 85% ethanol analysis solution, concentrate the analysis solution to a solid content of 35-40%, and place the concentrated solution to crystallize at 5°C. After ...

Embodiment 3

[0042] Put 25kg of tea products directly degreened without traditional processing into an extraction tank with a volume of 0.5T, spray evenly 75L of pure water at a temperature of 60°C from the top of the extraction tank, and soak for 60 minutes. Then spray 80L of pure water at 80°C evenly at a flow rate of 25L / h, and start discharging at a flow rate of 25L / h at the same time (the temperature of the feed liquid is cooled to below 25°C through a cooling device). Flow rate on AMBERLITE XAD4 column, column volume is 18L. After all the materials are loaded on the column, elute with 60L of pure water, and then analyze with 50L of 25% ethanol aqueous solution at a flow rate of 20L / h. After loading the column, elute with 40L of 30% ethanol aqueous solution, and finally analyze with 50L of 75% ethanol, collect the 75% ethanol analysis solution, concentrate the analysis solution to a solid content of 30-35%, and place the concentrated solution at 5°C to crystallize. After the crystals...

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Abstract

The invention relates to a method for extracting epigallocatechin gallate (EGCG) from tea raw materials. The method comprises the following steps: soaking tea raw materials in warm water, extracting, making the extract liquid go through a macroporous resin column, then making the extract liquid go through a polyamide resin column, condensing the obtained liquid, crystallizing, carrying out suction filtration, washing, and drying in vacuum so as to obtain the EGCG. Tea raw materials are soaked in warm water, water with a temperature higher than the soaking temperature is used to carry out spray-extraction, and then a continuous column chromatography method is adopted to purify the extract. The continuous column chromatography method comprises the following steps: loading macroporous resin into a column, making the extract liquid go through the column to obtain an ethanol analysis liquid with a concentration of 15 to 30%, then making the ethanol analysis liquid go through a polyamide resin column, using ethanol with a concentration of 70 to 85% to carry out elution so as to obtain an elution liquid of EGCG, removing ethanol from the elution liquid, and carrying out condensation and crystallization so as to obtain the finished EGCG product. The EGCG content of the product can reach 98% or more, and the yield can reach 80% or more.

Description

technical field [0001] The invention relates to the field of tea processing, in particular to a method for separating and preparing EGCG from tea. Background technique [0002] Tea leaves refer to the leaves of Camellia sinensis (Linn.) O. Kuntze, the plant of the genus Theaceae. my country is the origin of tea. Catechin belongs to the flavan-3-ol compounds and is the main active ingredient in tea. Among them, the content of catechin in summer and autumn tea is relatively high. [0003] Tea leaves mainly contain 4 kinds of catechin compounds, namely epicatechin (Epicatechin, EC), epicatechin-3-gallate (Epicatechin-3-gallate, ECG), epigallocatechin (Epigallocatechin, EGC) and Epigallocatechin-3-gallate (EGCG). [0004] EGCG, the component with the highest content of catechins, accounts for 9%-13% of the gross weight of green tea. Because of its special stereochemical structure, it has a series of biological activities such as anti-aging, anti-allergy, anti-inflammation, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 龚雨顺袁沛杨志辉刘仲华黄建安
Owner HUNAN AGRICULTURAL UNIV
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