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A kind of rabbit skin extract and its preparation method and application

An extract and rabbit skin technology, applied in the fields of immunology and biomedicine, can solve the problems of time-consuming, safety and reliability of extracts, environmental safety risks, time-consuming and labor-intensive, etc., achieve excellent technical effects, shorten extraction time, improve The effect of extraction efficiency

Active Publication Date: 2022-05-10
天津运三泽生物医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, it is time-consuming and labor-intensive to prepare such rabbit skin extracts using traditional techniques
For example, Chinese patent applications CN1493302A, CN103110663A, CN105163746A, CN106109498A, CN106573020A all disclose the extraction method of rabbit skin extract; However, in these extraction methods, only the time-consuming of the initial extraction step has reached 6-11 days, very time consuming
At the same time, in the traditional extraction method of rabbit skin extract, it is usually necessary to use organic solvents (such as phenol), which leads to potential risks in terms of the safety and reliability of the extract and environmental safety.

Method used

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  • A kind of rabbit skin extract and its preparation method and application
  • A kind of rabbit skin extract and its preparation method and application
  • A kind of rabbit skin extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1. Preparation of Rabbit Skin Extract

[0085] The pox-prone skin of rabbits (New Zealand white rabbits, body weight greater than 2.5 kg) inoculated intradermally with vaccinia virus Lister strain was collected, and frozen in a freezer at -20°C in advance for future use.

[0086] (A) Take out the frozen rabbit skin from the -20°C freezer, weigh 80g, cut it into small pieces of 2-3 cm, and place it in liquid nitrogen while cutting. After all the cuttings are completed, put the rabbit skin in a pulverizer and pulverize it into particles (75-200 μm). Place the crushed rabbit skin in a beaker, seal it with plastic wrap, and wait for it to thaw to room temperature (measure once every half an hour; under normal circumstances, after liquid nitrogen treatment, the rabbit skin can reach room temperature in 2.5-3 hours ). Subsequently, the rabbit skin was placed in a constant temperature incubator at 37±2° C. for 12 hours.

[0087] (B) Put the product obtained in (A...

Embodiment 2

[0132] Example 2. Evaluation of the effect of rabbit skin extract on promoting the proliferation of oligodendrocytes

[0133] In this example, oligodendrocytes were used to study the effect of the rabbit skin extract prepared in Example 1 on promoting proliferation.

[0134] Oligodendrocytes were extracted from the cerebral cortex of SD female rats within 48 hours after birth. After five passages, the morphology of oligodendrocytes was observed under a microscope. Subsequently, cells after passage 5 were plated in poly-lysine-coated well plates. On day 1 after plating, cultured cells were identified and characterized using an oligodendrocyte-specific antibody (anti-O4 antibody) and DAPI staining.

[0135] Experimental results such as Figures 3A-3C shown. Figure 3A The results of immunofluorescence detection of cultured cells with anti-O4 antibody are shown (fluorescent micrographs). The results showed that the cultured cells were positive for anti-O4 antibody and were o...

Embodiment 3

[0141] Example 3. Evaluation of the Effect of Rabbit Skin Extract on Promoting Proliferation of Bone Marrow Mesenchymal Stem Cells

[0142] In this example, bone marrow mesenchymal stem cells in the rat femur were used to study the effect of the rabbit skin extract prepared in Example 1 on promoting proliferation.

[0143] Bone marrow mesenchymal stem cells were extracted from femurs of wistar female rats within 48 hours after birth. After 5 passages, bone marrow mesenchymal stem cells were plated in a 96-well plate at a density of 5000 cells per well, and 7 experimental groups and 1 control group were set up, among which:

[0144] The cell culture medium that 7 experimental groups adopt is DMEM / F12 medium, wherein adds the rabbit skin extract that concentrates embodiment 1 to prepare respectively, makes its final concentration in culture medium be 0.2NU / ml, 0.5NU / ml respectively. ml, 1NU / ml, 1.5NU / ml, 2NU / ml, 2.5NU / ml, 3NU / ml;

[0145] The cell culture medium used in one co...

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Abstract

The application relates to a rabbit skin extract and its preparation method and use. In particular, the present application provides a method for preparing a rabbit skin extract, and the rabbit skin extract prepared by the method. In addition, the present application also provides a composition comprising the rabbit skin extract, and uses of the rabbit skin extract.

Description

technical field [0001] This application relates to the fields of immunology and biomedicine. In particular, the present application relates to a rabbit skin extract, as well as its preparation method and use. More specifically, the present application provides a method for preparing a rabbit skin extract, and the rabbit skin extract prepared by the method. In addition, the present application also provides a composition comprising the rabbit skin extract, and uses of the rabbit skin extract. Background technique [0002] The vaccinia virus-inoculated rabbit inflamed skin extract is an extract containing non-protein active substances isolated from inflamed skin tissue of rabbits inoculated with vaccinia virus. Such extracts are liquid in the extracted state, but can also be made solid by drying. It has been reported in the literature that such extracts can be used to treat low back pain, neck, shoulder and wrist syndrome, symptomatic neuralgia, itching associated with skin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/36A61P29/00A61P25/00A61P17/00A61P17/04A61P11/02A61P37/08A61P31/22A61P19/02A61P17/02A61P19/08
CPCA61K35/36
Inventor 冯世庆孔晓红姚雪冯翊坤李波孙超
Owner 天津运三泽生物医药科技有限公司
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