Preparation process and application of dendrobe small-molecular polysaccharide extract liquid
A polysaccharide extraction and preparation technology technology is applied in the field of dendrobium extraction to achieve the effects of good skin feeling, improving moisturizing effect and preventing skin aging
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Embodiment 1
[0053] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:
[0054] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;
[0055] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:150, soak for 0.5 hours, then extract it ultrasonically at 30°C for 0.5 hour, then place it in a heating mantle and heat it to 75°C while adding 1% of the weight of Dendrobium officinale powder Chitosan, open the cold circulating water and carry out hot reflux extraction for 2 hours, cool down to 40°C, add 1% cellulase of Dendrobium officinale powder weight, react, heat reflux extraction for 0.5 hour;
[0056] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal fil...
Embodiment 2
[0060] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:
[0061] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;
[0062] 2) Add the Dendrobium officinale powder into deionized water according to the ratio of material to liquid at 1:165, soak for 0.8 hours, then extract it ultrasonically at 35°C for 1 hour, then place it in a heating mantle and heat it to 80°C while adding 2% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 2.5 hours, cool to 45°C, add 3.5% cellulase by weight of Dendrobium officinale powder, and heat reflux extraction for 1 hour;
[0063] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal filtr...
Embodiment 3
[0065] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:
[0066] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;
[0067] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:180, soak for 1 hour, then extract it ultrasonically at 40°C for 1.5 hours, then place it in a heating mantle and heat it to 85°C while adding 3% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 3 hours, cool down to 50°C, add 5% cellulase by weight of the dry stem of Dendrobium officinale, and extract by reaction heat reflux for 1.5 hours;
[0068] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry o...
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