Preparation process and application of dendrobe small-molecular polysaccharide extract liquid

Abstract

The invention discloses a preparation process of a dendrobe small-molecular polysaccharide extract liquid. The process includes: a) crushing dendrobium officinale and screening the dendrobium officinale through a sieve in 60 meshes, and adding deionized water according to material-to-liquid ratio of 1:150-1:180 by weight to soak the dendrobium officinale for 0.5-1 h; b) performing ultrasonic extraction for 0.5-1.5 h, temperature controlled at 30-40 DEG C; c) performing reflux extraction at 75-85 DEG C for 2-3 h and meanwhile adding chitosan accounting for 1-3 wt% of the dendrobium officinale,and continuously stirring and naturally cooling the extract to 40-50 DEG C; d) adding cellulase accounting for 1-5 wt% of the dendrobium officinale, performing heat reflux extraction for 0.5-1.5 h, heating the mixture to 75-85 DEG C and maintaining the temperature for 3-7 min; e) cooling and filtering the mixture to prepare the extract liquid. The dendrobe extract liquid contains a large quantityof dendrobe small-molecular polysaccharides and can deeply enter the basal layer of skin, so that the deep moisturizing effect of the dendrobe polysaccharide on skin is greatly enhanced. The dendrobeextract liquid is natural and safe and effectively achieves multiple moisturizing effects on skin.

Description

technical field

[0001] The invention relates to a preparation process and application of dendrobium small-molecule polysaccharide extract, belonging to the technical field of dendrobium extraction. Background technique

[0002] Lack of moisture in the skin can lead to various skin problems, such as dryness, yellowing, dullness, sagging, wrinkles, aging, etc. When the skin is hydrated, it will appear plump, smooth, moist and shiny, and the skin will be firm Full of elasticity.

[0003] There are multiple ways to hydrate the skin. Most hydrating methods directly supply the water needed by the stratum corneum cells of the skin and moisturize the skin. If you want long-term hydration and moisturizing, you need more nutrients to penetrate the stratum corneum and reach the bottom layer of the skin to achieve deep hydrating and moisturizing effects.

[0004] Dendrobium is rich in Dendrobium polysaccharides, the molecular weight of which varies greatly. The general extraction meth...

Examples

Embodiment 1

[0053] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0054] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0055] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:150, soak for 0.5 hours, then extract it ultrasonically at 30°C for 0.5 hour, then place it in a heating mantle and heat it to 75°C while adding 1% of the weight of Dendrobium officinale powder Chitosan, open the cold circulating water and carry out hot reflux extraction for 2 hours, cool down to 40°C, add 1% cellulase of Dendrobium officinale powder weight, react, heat reflux extraction for 0.5 hour;

[0056] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal fil...

Embodiment 2

[0060] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0061] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0062] 2) Add the Dendrobium officinale powder into deionized water according to the ratio of material to liquid at 1:165, soak for 0.8 hours, then extract it ultrasonically at 35°C for 1 hour, then place it in a heating mantle and heat it to 80°C while adding 2% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 2.5 hours, cool to 45°C, add 3.5% cellulase by weight of Dendrobium officinale powder, and heat reflux extraction for 1 hour;

[0063] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal filtr...

Embodiment 3

[0065] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0066] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0067] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:180, soak for 1 hour, then extract it ultrasonically at 40°C for 1.5 hours, then place it in a heating mantle and heat it to 85°C while adding 3% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 3 hours, cool down to 50°C, add 5% cellulase by weight of the dry stem of Dendrobium officinale, and extract by reaction heat reflux for 1.5 hours;

[0068] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry o...

technical field

[0001] The invention relates to a preparation process and application of dendrobium small-molecule polysaccharide extract, belonging to the technical field of dendrobium extraction. Background technique

[0002] Lack of moisture in the skin can lead to various skin problems, such as dryness, yellowing, dullness, sagging, wrinkles, aging, etc. When the skin is hydrated, it will appear plump, smooth, moist and shiny, and the skin will be firm Full of elasticity.

[0003] There are multiple ways to hydrate the skin. Most hydrating methods directly supply the water needed by the stratum corneum cells of the skin and moisturize the skin. If you want long-term hydration and moisturizing, you need more nutrients to penetrate the stratum corneum and reach the bottom layer of the skin to achieve deep hydrating and moisturizing effects.

[0004] Dendrobium is rich in Dendrobium polysaccharides, the molecular weight of which varies greatly. The general extraction meth...

Examples

Embodiment 1

[0053] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0054] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0055] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:150, soak for 0.5 hours, then extract it ultrasonically at 30°C for 0.5 hour, then place it in a heating mantle and heat it to 75°C while adding 1% of the weight of Dendrobium officinale powder Chitosan, open the cold circulating water and carry out hot reflux extraction for 2 hours, cool down to 40°C, add 1% cellulase of Dendrobium officinale powder weight, react, heat reflux extraction for 0.5 hour;

[0056] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal fil...

Embodiment 2

[0060] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0061] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0062] 2) Add the Dendrobium officinale powder into deionized water according to the ratio of material to liquid at 1:165, soak for 0.8 hours, then extract it ultrasonically at 35°C for 1 hour, then place it in a heating mantle and heat it to 80°C while adding 2% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 2.5 hours, cool to 45°C, add 3.5% cellulase by weight of Dendrobium officinale powder, and heat reflux extraction for 1 hour;

[0063] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry out centrifugal filtr...

Embodiment 3

[0065] A preparation process of dendrobium small molecule polysaccharide extract, comprising the following steps:

[0066] 1) Grinding the dried stem of Dendrobium candidum into fine powder with a grinder, passing through a 60-mesh sieve, sifting through the net and mixing the finely crushed Chinese medicine;

[0067] 2) Add the Dendrobium officinale powder in deionized water according to the ratio of material to liquid at 1:180, soak for 1 hour, then extract it ultrasonically at 40°C for 1.5 hours, then place it in a heating mantle and heat it to 85°C while adding 3% of the weight of Dendrobium officinale For chitosan, turn on the cold circulating water and carry out heat reflux extraction for 3 hours, cool down to 50°C, add 5% cellulase by weight of the dry stem of Dendrobium officinale, and extract by reaction heat reflux for 1.5 hours;

[0068] 3) Cool the obtained reflux extraction liquid to normal temperature, let it stand for 20 minutes, take the supernatant and carry o...

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