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Composite protein gel and preparation method thereof

A composite protein and gel technology, which is applied in the field of composite protein gel and its preparation, can solve the problems of poor stability of fillers, stiff skin, high absorption rate, etc., and achieve long maintenance time, small immune rejection and strong regenerative ability Effect

Pending Publication Date: 2019-04-16
四川伍衍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a composite protein gel and its preparation method. The protein gel can effectively solve the problems of hyaluronic acid or autologous fat that easily cause skin stiffness, collapse, and in vivo absorption rate during filling. Problems with high and poor filling stability

Method used

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  • Composite protein gel and preparation method thereof

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Effect test

Embodiment 1

[0027] A kind of composite protein gel, its preparation method, comprises the following steps:

[0028] (1) Collect umbilical cord blood with a blood collection tube with 3.8% sodium citrate anticoagulant, and the volume ratio of sodium citrate to umbilical cord blood is 1:13;

[0029] (2) centrifuging the umbilical cord blood in step (1) at room temperature with a centrifugal acceleration of 300g for 10min;

[0030] (3) absorb the upper plasma layer in the centrifuged blood in step (2), and place it in an ultra-low temperature refrigerator at -80° C. for rapid freezing, and the freezing time is 24 hours;

[0031] (4) The plasma frozen in step (3) is taken out, and freeze-dried at a temperature of -55° C. and a pressure of 50 Pa to obtain freeze-dried plasma;

[0032] (5) Mix the freeze-dried plasma prepared in step (4) with calcium chloride injection at a concentration of 20 μmol / L at a volume ratio of 1:0.5, then react in a water bath at 60°C for 5 minutes, and take it out ...

Embodiment 2

[0034] A kind of composite protein gel, its preparation method, comprises the following steps:

[0035] (1) Collect autologous venous blood with a blood collection tube with 3.8% sodium citrate anticoagulant, and the volume ratio of sodium citrate to autologous venous blood is 1:13;

[0036] (2) centrifuging the autologous venous blood in step (1) at room temperature with a centrifugal acceleration of 700g for 20min;

[0037] (3) absorb the upper plasma layer in the centrifuged blood in step (2), and place it in an ultra-low temperature refrigerator at -80° C. for rapid freezing, and the freezing time is 72 hours;

[0038] (4) The plasma frozen in step (3) is taken out, and freeze-dried at a temperature of -65° C. and a pressure of 100 Pa to obtain freeze-dried plasma;

[0039] (5) Mix the freeze-dried plasma prepared in step (4) with calcium chloride injection at a concentration of 50 μmol / L at a volume ratio of 1:2, then react in a water bath at 90° C. for 15 minutes, and t...

Embodiment 3

[0041] A method for preparing a composite protein gel, comprising the following steps:

[0042] (1) Collect umbilical cord blood with a blood collection tube with 3.8% sodium citrate anticoagulant, and the volume ratio of sodium citrate to umbilical cord blood is 1:13;

[0043] (2) centrifuging the umbilical cord blood in step (1) at room temperature with a centrifugal acceleration of 500g for 15min;

[0044] (3) Absorb the upper plasma layer in the centrifuged blood in step (2), and place it in an ultra-low temperature refrigerator at -80° C. for rapid freezing, and the freezing time is 48 hours;

[0045] (4) The plasma frozen in step (3) is taken out, and freeze-dried at a temperature of -65° C. and a pressure of 70 Pa to obtain freeze-dried plasma;

[0046] (5) Mix the freeze-dried plasma prepared in step (4) with calcium chloride injection at a concentration of 30 μmol / L at a volume ratio of 1:1.5, then react in a water bath at 80°C for 10 minutes, and take it out to obta...

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Abstract

The invention discloses a composite protein gel and a preparation method thereof. The preparation method comprises the steps of collecting blood by using a blood collection tube with an anticoagulant;centrifuging the blood for 10-20min at room temperature at the centrifugal acceleration of 300-700g; transferring the centrifuged blood to a biosafety cabinet, absorbing an upper plasma layer and freezing the upper plasma layer in an ultra-low temperature freezer at -80 DEG C for 24-72h; then removing the frozen plasma, lyophilizing the plasma at the temperature of 55-65 DEG C and the pressure of50-100Pa to obtain the frozen dry blood plasma; adding a calcium chloride injection to the frozen dry plasma at the volume ratio of 1:0.5-2 and conducting reaction in a water bath of 60-90 DEG C for5-15min and taking out to obtain the protein gel. The protein gel can effectively solve the problems of skin rigidity and collapse, high absorption rate in the body and poor filler stability of hyaluronic acid and autologous fat.

Description

technical field [0001] The invention belongs to the field of beauty products, and in particular relates to a composite protein gel and a preparation method thereof. Background technique [0002] In recent years, fillers applied to the skin are mainly of the following two types: hyaluronic acid and fat. [0003] Hyaluronic acid (HA) has been widely used in the field of skin treatment as a dermal filler. Hyaluronic acid can enhance the elasticity of soft tissues, promote the proliferation and differentiation of epidermal cells, and have high biocompatibility. However, hyaluronic acid has the disadvantages of causing skin stiffness and collapse after injection, and has a high absorption rate in the body. At the same time, it does not have the ability to resist skin aging and promote tissue regeneration. [0004] Autologous fat filling is also a technique with more clinical applications. The vascular stromal tissue (SVF) produced by autologous fat after initial treatment in vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/52A61L27/60
CPCA61L27/3616A61L27/3687A61L27/3691A61L27/52A61L27/60C08L89/00
Inventor 李俊周丽薇石玥李升岐徐能飞
Owner 四川伍衍生物科技有限公司
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