Detection kit for indirect blood coagulation antibody of macrococcus caseolyticus as well as preparation and application of detection kit

Abstract

The invention discloses a detection kit for an indirect blood coagulation antibody of macrococcus caseolyticus as well as preparation and application of the detection kit. The kit comprises (1), indirect blood coagulation antigen of the macrococcus caseolyticus, (2), standard positive serum, (3) standard negative serum, and (4), diluting liquid. According to the detection kit for the indirect blood coagulation antibody of the macrococcus caseolyticus as well as the preparation and the application of the detection kit, the kit has the beneficial effects of being simple and convenient to operate, and is suitable for being widely popularized and used in various basic veterinary related units and farms; the kit only needs to use several simple consumable materials and a temperature box when asample is detected, and can be used even in a livestock farm with relatively simple conditions; the result can be judged directly, only by eyes can judge; an operator does not need professional knowledge and does not need to be subjected to professional training, and can detect only by referring to a specification; and the kit is low in coat, economical and practical, and can be accepted by all kinds of users.

Description

technical field [0001] The invention belongs to the field of detection kits, and in particular relates to a detection kit for indirect hemagglutination antibody of E. caseinus and its preparation and application. Background technique [0002] Macrococcus caseolyticus (Macrococcus caseolyticus, MCSD) belongs to the Megacoccus genus, which includes 7 species, and Macrococcus caseolyticus is the representative species. The bacteria can be specifically isolated from animal skins and animal foods, such as milk, raw beef, chicken, ham, etc., and can also be isolated from Cantonese-style sausage, a traditional meat product in my country. The bacteria can cause fish disease, and has been isolated from fish such as flounder, mackerel, and California perch, which has brought harm to aquaculture. In addition, M. casein can also cause dogs to develop symptoms of rhinitis. The bacterium also carries the methicillin-resistant SCCmec element (mecB-carrying SCCmecelement), showing high re...

AI Technical Summary

Problems solved by technology

For the first time, our research team isolated multiple strains of M. caseinolyticus from ducks, and the isolated strains were all highly resistant to ciprofloxacin, suggesting that ciprofloxacin is ineffective in the treatment of clinical animals caused by M. caseinolyticus

[0003] At present, there are very few research reports on M. caseolyticus at home and abroad. There is only one full sequence of M. The serum antibody detection technology has not been reported at home and abroad

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