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Fruit disease control method based on saccharomyces cerevisiae cell wall product induction activity resistance

A technology of Saccharomyces cerevisiae cells and inducing resistance, which is applied in the preservation of food ingredients as antimicrobials, food science, preservation of fruits and vegetables, etc. Low cost, high cost performance, good social and ecological benefits

Inactive Publication Date: 2019-04-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale use of chemical fungicides seriously pollutes the environment and is detrimental to human health
On the other hand, if a single chemical fungicide is used for a long time, pathogenic bacteria may evolve to form drug resistance, which directly leads to the fact that some fungicides currently allowed to be used are less and less effective in disease control, such as thiabendazole and imazalil fungicides

Method used

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  • Fruit disease control method based on saccharomyces cerevisiae cell wall product induction activity resistance
  • Fruit disease control method based on saccharomyces cerevisiae cell wall product induction activity resistance
  • Fruit disease control method based on saccharomyces cerevisiae cell wall product induction activity resistance

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Experimental program
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Effect test

Embodiment 1-1

[0042] Embodiment 1-1, the preparation that suppresses fruit postharvest disease by inducing resistance,

[0043] Saccharomyces cerevisiae with the preservation number CGMCC No.2.3854 was stored at low temperature (4°C) in NYDA medium (beef extract 8g, yeast extract powder 5g, glucose 10g, agar 20g, water to 1000mL, at 0.1MPa, 121 Under high pressure steam sterilization at ℃ for 20min), take out after activation, culture in NYDA medium at 25℃ for 48h, repeat subculture twice, use the inoculation loop to inoculate the activated yeast into NYDB, at 200rpm, After culturing at 28° C. for 24 hours, the culture medium was collected, centrifuged at 3000 g for 10 min, washed with sterile distilled water three times to remove residual culture medium, and the precipitate was collected by centrifugation to obtain Saccharomyces cerevisiae cells.

[0044] Saccharomyces cerevisiae was resuspended in 0.2mol / L phosphate buffer (PBS, pH 8.0) to prepare 1×10 8 cell / mL bacterial suspension. Ad...

Embodiment 2-1

[0047] Embodiment 2-1, the preparation that suppresses fruit postharvest disease by inducing resistance:

[0048] Saccharomyces cerevisiae with the preservation number CGMCC No.2.3854 was stored at low temperature (4°C) in NYDA medium (beef extract 8g, yeast extract powder 5g, glucose 10g, agar 20g, water to 1000mL, at 0.1MPa, 121 Under the condition of high pressure steam sterilization for 20min at ℃), take it out during activation, and culture it in NYDA medium at 25℃ for 48h. After culturing at 28° C. for 24 hours, the culture medium was collected, centrifuged at 3000 g for 10 min, washed with sterile distilled water three times to remove residual culture medium, and the precipitate was collected by centrifugation to obtain Saccharomyces cerevisiae cells.

[0049] Saccharomyces cerevisiae was resuspended in 0.2mol / L phosphate buffer (PBS, pH 8.0) to prepare 1×10 8 cell / mL bacterial suspension. Add 0.5 g of acid-washed glass beads (Sigma, particle size 425-600 μm) to 0.5 m...

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Abstract

The invention provides a harvested fruit disease control preparation based on induction activity resistance, which consists of a saccharomyces cerevisiae cell wall product and water; the mass concentration of the saccharomyces cerevisiae cell wall product in the preparation is 0.1%-1%. The invention also provides a harvested fruit disease control method based on the induction activity resistance,which comprises the following steps of: inoculating the preparation at a wound of a fruit so that the preparation completely covers the wound of the fruit; and putting the fruit into a container for storage in a sealed state after the preparation at the wound of the fruit is naturally air-dried. The saccharomyces cerevisiae cell wall (1-3)-beta-D-glucan and saccharomyces cerevisiae cell wall beta-D1,6-glucan are rich in resources, low in cost, high in cost performance, easy to produce and transport and biodegradable.

Description

technical field [0001] The invention relates to the technical field of fruit postharvest disease prevention and control, in particular to a biological fresh-keeping technology for inducing fruit resistance through dextran, a cell wall product of Saccharomyces cerevisiae. Background technique [0002] Fruit is an important source of vitamins, minerals and dietary fiber. It is an essential food for people to maintain their health and improve their nutrition. It is the agricultural product second only to food in the world. However, the losses caused by postharvest diseases are huge. According to general conservative estimates, the postharvest losses of fruits in my country are about 20% to 25%, which seriously affects the market supply and farmers' economic benefits. There are many factors leading to the postharvest loss of fruit, and the rot caused by the infection of pathogenic bacteria is the main reason for the postharvest loss of fruit, among which mold is the main pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154C08B37/02
CPCA23B7/154A23V2002/00C08B37/0003C08B37/0024A23V2200/10
Inventor 余挺孙翠金丽飞林明黄伊宁蔡怡婷
Owner ZHEJIANG UNIV
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