A kind of preparation method of egg yolk antibody of Pasteurella avian capsule antigen group A

A technology of Pasteurella fowl and capsular antigen, which is applied in the field of preparation of egg yolk antibody of Pasteurella fowl capsular antigen A group, can solve the problems of narrow immune spectrum, short protection period, low protection rate and the like, and achieves simple steps and low consumption. Short time, good vitality

Active Publication Date: 2019-09-06
广东渔跃生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most effective way of preventing and controlling this disease is vaccine immunization. The avian Pasteurella disease vaccine commonly used at home and abroad is an inactivated vaccine at present, but the conventional inactivated vaccine immunization has a low protection rate of about 80%, and the immune spectrum is narrow (only for The same-type strains have better protection), short protection period, high cost and other disadvantages

Method used

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  • A kind of preparation method of egg yolk antibody of Pasteurella avian capsule antigen group A
  • A kind of preparation method of egg yolk antibody of Pasteurella avian capsule antigen group A

Examples

Experimental program
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Embodiment 1

[0016] Preparation:

[0017] (1) Subcutaneously inject 2 mL of Pasteurella avian capsular antigen group A inactivated vaccine to Hai-Line brown chickens aged 12 to 15 weeks for a total of 3 times, and the time interval between two adjacent immunizations is 10 days. Collect eggs 3 days after one immunization to obtain highly immune eggs;

[0018] (2) Disinfect the surface of the high-free eggs with 75% alcohol, wash them with water, separate the egg yolks, pass through a 60-mesh nylon sieve to remove the vitelline membrane and frenulum egg yolks, and break them to obtain egg yolk liquid;

[0019] (3) Mix the egg yolk liquid obtained in step (2) with purified water at a volume ratio of 1:2, and after stirring evenly, add L-ascorbic acid, 4% (m / v) propylene glycol and 3% (m / v) dextran sulfate, after fully stirring, place at 8°C for 2h, centrifuge at 10000r / min at 8°C for 20min, and collect the supernatant;

[0020] (4) Filter and sterilize the supernatant collected in step (3) ...

Embodiment 2

[0022] Preparation:

[0023] (1) Subcutaneously inject 2 mL of Pasteurella avian capsular antigen group A inactivated vaccine to Hai-Line brown chickens aged 12 to 15 weeks for a total of 3 times, and the time interval between two adjacent immunizations is 10 days. Collect eggs 3 days after one immunization to obtain highly immune eggs;

[0024] (2) Disinfect the surface of the high-free eggs with 75% alcohol, wash them with water, separate the egg yolks, pass through a 60-mesh nylon sieve to remove the vitelline membrane and frenulum egg yolks, and break them to obtain egg yolk liquid;

[0025] (3) Mix the egg yolk liquid obtained in step (2) with purified water at a volume ratio of 1:4, and after stirring evenly, add L-ascorbic acid, 8% (m / v) propylene glycol and 6% (m / v) dextran sulfate, after fully stirring, place at 2°C for 4h, centrifuge at 10000r / min at 5°C for 30min, and collect the supernatant;

[0026] (4) Filter and sterilize the supernatant collected in step (3) ...

Embodiment 3

[0028] Preparation:

[0029] (1) Subcutaneously inject 2 mL of Pasteurella avian capsular antigen group A inactivated vaccine to Hai-Line brown chickens aged 12 to 15 weeks for a total of 3 times, and the time interval between two adjacent immunizations is 10 days. Collect eggs 3 days after one immunization to obtain highly immune eggs;

[0030] (2) Disinfect the surface of the high-free eggs with 75% alcohol, wash them with water, separate the egg yolks, pass through a 60-mesh nylon sieve to remove the vitelline membrane and frenulum egg yolks, and break them to obtain egg yolk liquid;

[0031] (3) Mix the egg yolk liquid obtained in step (2) with purified water at a volume ratio of 1:4, and after stirring evenly, add L-ascorbic acid, 6% (m / v) propylene glycol and 4% (m / v) dextran sulfate, after fully stirring, place at 4°C for 3 hours; centrifuge at 11000r / min at 5°C for 30 minutes, and collect the supernatant;

[0032] (4) Filter and sterilize the supernatant collected in...

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Abstract

The invention discloses a preparation method of a pastevula mulfocida capsular antigen A group yolk antibody. The method includes the following steps that (1) a pastevula mulfocida capsular antigen Agroup inactivated vaccine is used for immunizing reproductive-age hens to obtain high-immune eggs; (2) high-immune egg yolks are collected, yolk membranes are removed, and crushing is carried out to obtain yolk liquid; (3) the yolk liquid obtained in step (2) and purified water are mixed in a volume ratio of 1:(2-4) and stirred to be uniform, L-ascorbic acid with a final concentration of 1-3% (m / v), propylene glycol with a final concentration of 4-8% (m / v) and dextran sulfate with a final concentration of 3-6% (m / v) are added, after sufficient stirring, the mixture is placed for 2-4 h at a lowtemperature, low-temperature centrifugation is carried out, and supernate is collected; (4) the supernate collected in step (3) is subjected to filtration sterilization to obtain yolk antibody liquid. The preparation method of the pastevula mulfocida capsular antigen A group yolk antibody includes simple steps, has high efficiency and yield and is economical and applicable.

Description

technical field [0001] The invention relates to a method for preparing egg yolk antibody of Pasteurella avian capsule antigen group A. Background technique [0002] Pasteurellosis multocida, also known as fowl cholera, is an acute, contact septicemia infectious disease caused by Pasteurella multocida (PM), which is distributed all over the world and has a great impact on the poultry industry. The development has a serious impact. According to the differences in antigenic components between strains, Pasteurella multocida is divided into multiple serotypes. In recent years, strain A is the main popular serotype in China. The most effective way of preventing and controlling this disease is vaccine immunization. The avian Pasteurella disease vaccine commonly used at home and abroad is an inactivated vaccine at present, but the conventional inactivated vaccine immunization has a low protection rate of about 80%, and the immune spectrum is narrow (only for Isotype strains have be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/285C07K16/02
Inventor 张毓金严悌昆谢秉超杨球
Owner 广东渔跃生物技术有限公司
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