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34results about How to "Retain vitality" patented technology

Jackfruit yoghourt pudding and preparation method thereof

The invention discloses a jackfruit yoghourt pudding and a preparation method thereof. Each 100 parts by weight of jackfruit yoghourt pudding comprises the following components: 60-80 parts by weight of milk, 1-5 parts by weight of whole milk powder, 0.1-2 parts by weight of durian pulp, 1-6 parts by weight of single cream, 0.5-1.2 parts by weight of sweetening agent, 0.5-1.2 parts by weight of compound emulsifying thickener, 0.05-0.15 part by weight of essence, 0.02-0.03 part by weight of mixed bacterium powder of streptococcus thermophilus and lactobacillus bulgaricus, and the balance of water. The jackfruit yoghourt pudding has the advantages of high nutritive value, thin and smooth taste, uniformity and stability, and long expiration date. The preparation method has the advantages of simplifying process procedures, being suitable for industrialized production, maximally ensuring strain activity, enhancing nutrition and function characteristics of the jackfruit yoghourt pudding, and being more beneficial to the intestinal health of a human body.
Owner:HEBEI BROS ILONG FOOD TECH LLC

Bacterial strain for highly yielding of nattokinase, and method for preparing natto through bacterial strain

The invention belongs to the technical field of bioengineering, and provides a bacterial strain for highly yielding of nattokinase, and a method for preparing natto through the bacterial strain. The involved bacterial strain for highly yielding of the nattokinase is identified through 16SrDNA to be Bacillus subtilis, the code of the bacterial strain is YJY19-09, the bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.18402. The bacterial strain is applied to production of the natto, thus the natto with the content of nattokinase being 4000-5000 IU / g can be obtained, and the content is higher than that of commercially available nattokinase.
Owner:CHAMBROAD CHEM IND RES INST CO LTD

Production process for tobacco bacterial wilt resistant compound microbial agent

The invention provides a production process for a tobacco bacterial wilt resistant compound microbial agent. Fertilizer comprises the raw materials of 15-20 parts of bacillus mucilaginosus, 15-20 parts of trichoderma harzianum, 15-20 parts of streptomyces microflavus, 10-35 parts of chitosan oligosaccharide, 10-15 parts of vegetable oil sludge and 10-15 parts of plant ash. By adopting the production process of bacterium preparation, dosing, compound bacterium pelletizing, first-layer vegetable oil sludge adhesive-smearing, second-time plant ash adhesive-smearing and third-time chitosan oligosaccharide adhesive-smearing, the three probiotics are combined together and meanwhile separated from the antibacterial component, namely, the chitosan oligosaccharide, so that the respective effects are given to play to the maximum extent. Through the antagonism effect and the killing effect of microorganisms on bacterial wilt bacteria, and induced resistance of the chitosan oligosaccharide to tobacco, tobacco bacterial wilt is prevented and treated; and the advantages of non-pollution, environmental friendliness, low cost and lasting effect are achieved.
Owner:烟台丰成农业科技有限公司

F85-20 protein, coding gene thereof and application of F85-20 protein as beta-glucosidase

The invention discloses an F85-20 protein, a coding gene thereof and an application of the F85-20 protein as beta-glucosidase. The invention provides the protein shown in the following 1) or 2): 1) a protein formed by amino acid residues shown in a sequence 2 in a sequence table; 2) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 2 in the sequence table, has the same functions as the protein shown in 1) and is derived from the protein shown in 1). Experiments prove that new beta-glucosidase F85-20 can still retain 80% of activity after being incubated at 50 DEG C for two hours under the conditions that p-nitrophenol glucoside (pNPG) is taken as the substrate and the pH value is 6.0, and the optimum temperature is 65 DEG C; new beta-glucosidase F85-20 can retain more than 70% of residual activity in a pH value range of 4.0-7.5 after being incubated in buffer solutions with different pH values for 24 hours at 4 DEG C.
Owner:PEKING UNIV

Yogurt jam pudding containing active probiotics and preparation method thereof

The present invention discloses a yogurt jam pudding containing active probiotics and a preparation method thereof. The yogurt jam pudding comprises the following components in parts by weight: 28-32parts of white granulated sugar, 90-110 parts of high fructose corn syrup, 25-35 parts of glucose monohydrate, 10-15 parts of pudding powder, 20-28 parts of whole milk powder, 15-20 parts of skim milkpowder, 1.8-2.5 parts of essences, 2.0-2.5 parts of citric acid, 1.4-1.8 parts of malic acid, 4.2-4.6 parts of potassium sorbate, 2.0-3.0 parts of titanium dioxide, 660-700 parts of softened water, 18-22 parts of a lactic acid bacterium fermented raw solution and 75-85 parts of a blueberry, strawberry and cranberry jam. The yogurt jam pudding is simplified in processing flows during the preparation. The milk and other materials in an early stage are sterilized, the sterilized materials are cooled, the cooled materials are inoculated with bacteria, then the sterile jam is added, then the materials are subjected to a sterile filling method, the pudding product contains the probiotics, and the preparation method extends shelf life of the product, enables the shelf life of the yogurt jam pudding to be extended to 45 days, and can realize industrial production.
Owner:湖南新中意食品有限公司

Toning lotion composition with effects of improving skin color and brightening skin and preparation method thereof

The invention discloses a toning lotion composition with effects of improving skin color and brightening skin and a preparation method thereof. The toning lotion composition comprises, by weight, 1-35% of a skin color brightening composition, 5-30% of a humectant, 0-30% of a skin conditioner, 0-10% of a thickening agent, 0.01-2% of a chelating agent, 0.01-1% of a solubilizer, a proper amount of a pH conditioning agent, a proper amount of an antiseptic, 0.01-1.0% of daily essence and the balance deionized water. The skin color brightening composition comprises rice fermentation product filtrate, a Kappaphycus longissima extract and an apple fruit / Cryptotaenia harlandii / rose cell culture extract. The above multiple functional components have effects of preserving moisture, diminishing inflammation and removing free radicals, can directly reach the skin bottom and balance skin and make the skin surface fresh and bright. Through scientific combination of the raw materials and synergism of the functional components, the toning lotion composition realizes safe and effective skin color balance and whitening and brightening effects.
Owner:湖北省麦诗特生物科技有限公司

Production process for potato bacterial wilt resistant compound microbial agent

The invention provides a production process for a potato bacterial wilt resistant compound microbial agent. Fertilizer comprises the raw materials of 15-20 parts of bacillus mucilaginosus, 15-20 parts of trichoderma harzianum, 15-20 parts of streptomyces microflavus, 10-35 parts of chitosan oligosaccharide, 10-15 parts of vegetable oil sludge and 10-15 parts of plant ash. By adopting the production process of bacterium preparation, dosing, compound bacterium pelletizing, first-layer vegetable oil sludge adhesive-smearing, second-time plant ash adhesive-smearing and third-time chitosan oligosaccharide adhesive-smearing, the three probiotics are combined together and meanwhile separated from the antibacterial component, namely, the chitosan oligosaccharide, so that the respective effects are given to play to the maximum extent. Through the antagonism effect and the killing effect of microorganisms on bacterial wilt bacteria, and induced resistance of the chitosan oligosaccharide to potatoes, potato bacterial wilt is prevented and treated; and the advantages of non-pollution, environmental friendliness, low cost and lasting effect are achieved.
Owner:烟台丰成农业科技有限公司

Seed process technique in space breeding

The invention belongs to the technical field of space breeding, in particular relates to a seed processing technology in the space breeding, and the technology includes seed selection, package, storage, space loading and mutagenicity of seed, seed culture after the space loading and mutagenicity, and seedling selection, wherein, a syncopation processing is performed after seed selection and before package: a part with an embryo occupying one third of the whole seed is reserved, and the rest parts are ablated. Through the reasonable syncopation processing for the seed, the method of the invention greatly lightens the weight of the loading seed, at the same time, reserves the vitality of the seed and reduces the loading cost; in addition, the problem that sowing cannot be performed in season is solved through improving the breeding technology of the seed after space loading and mutagenicity; thereby providing fine support for the wide range of application of the space breeding technology.
Owner:河南省科学院同位素研究所有限责任公司

F52-6 protein and application of coding gene of F52-6 protein and hydrolyzed xylan

The invention discloses F52-6 protein and application of a coding gene of the F52-6 protein and hydrolyzed xylan. The protein disclosed by the invention is protein (1) or (2), wherein the protein (1) is formed by amino acid residues shown in a sequence 2 in a sequence table, and the protein (2) is formed through enabling a residue sequence of an amino acid sequence of the sequence 2 in the sequence table to be subjected to the substitution and / or deletion and / or addition of one or more amino acid residues and is protein which has the same functions and is derived from the protein (1). According to the F52-6 protein and the application of the coding gene of the F52-6 protein and the hydrolyzed xylan, proved by experiments, the optimal temperature for novel xylanase F52-6 is 75 DEG C under the conditions of taking xylan as a substrate and being in the pH value of 5.0, and activity of 82.3% can still be maintained after the novel xylanase F52-6 is incubated for 2 hours at the temperature of 70 DEG C; over 80% residual activity can be maintained in the pH value of 4.0-12.0 after the novel xylanase F52-6 is incubated for 24 hours in buffer solutions with different pH values at the temperature of 4 DEG C.
Owner:PEKING UNIV

Preparation method of fermented flour

The invention discloses a preparation method of fermented flour. The preparation method comprises the steps of enzymolysis, fermentation, freeze drying and the like. The fermented flour can be used asa natural baking raw material, completely replaces traditional sour dough, is safe to eat, green and healthy, and enables baked wheaten food to have both nutrition and delicious taste. Meanwhile, thefermented flour is rich in proteins, minerals, trace elements, active microbial components and the like, and is rich in nutrition. The fermented flour raw material is applied to the food industry, sothat the limitations of short storage life, difficulty in transportation and the like of fresh sour dough are avoided, flavor substances and active ingredients in the fresh sour dough are reserved tothe maximum extent, and the flavor and the performance of the product are guaranteed. And the flour can be directly mixed with common flour for use, so that the use method is simplified, a new way isdeveloped for food safety in China, and the flour has a good application prospect.
Owner:广西朗盛食品科技有限公司

Water-soluble microbial agent for promoting banana growing, preparation method and application thereof

The invention discloses a water-soluble microbial agent for promoting banana growing, a preparation method and application thereof. The compound contains six microorganisms, including pseudomonas aeruginosa, serratia marcescens, burkholderia tuberum, pseudomonas fluorescens, bacillus subtilis and trichoderma viride. The preparation method comprises the following steps of: preparing a water-soluble carbon source from rice flour processing wastes; culturing the six bacteria in the water-soluble carbon source, thereby obtaining six liquid microbial agents; and airing and mixing the six liquid microbial agents, thereby obtaining the water-soluble compound microbial agent. The invention relates to the technical fields of waste utilization, agricultural microorganisms, ecological planting and microbial agents. According to the water-soluble microbial agent for promoting banana growing, the preparation method and the application thereof, the rice flour wastes can be recycled, the industrial production cost can be lowered, the waste of waste resources can be avoided, the environmental pollution caused by the wastes can be reduced, the yield of banana can be increased and the plant diseases and insect pests of banana can be reduced.
Owner:南宁市拜欧生物工程有限责任公司

Durian yoghourt pudding

The invention discloses a durian yoghurt pudding. The durian yoghurt pudding is prepared from, by weight, 60-80 parts of milk, 1-5 parts of whole milk powder, 0.1-2 parts of durian pulp, 1-6 parts ofsingle cream, 0.5-1.2 parts of a sweetening agent, 0.5-1.2 parts of a compound emulsifying thickener, 0.05-0.15 part of essence, 0.02-0.03 part of mixed bacteria powder of streptococcus thermophilus and lactobacillus bulgaricus and the balance water, wherein the total weight parts are 100. The durian yoghurt pudding has the advantages of being high in nutritive value, fine in taste, smooth, uniform, stable and long in shelf life. The preparation method simplifies the technological process and is suitable for industrial production, the strain vitality is ensured to the greatest extent, the nutritional and functional characteristics of the product are enhanced, and the product is more beneficial to the human intestinal health.
Owner:孙晓菲

Method for preparing chitosan oligosaccharide by enzymolysis of supercritical carbon dioxide fluid

The invention provides a method for preparing chitosan oligosaccharide by enzymolysis of supercritical carbon dioxide fluid. The method comprises the following steps: grinding and spray-drying a chitosan solution to obtain pretreated chitosan powder; adding a sodium alginate solution in a glutaraldehyde solution, stirring at a room temperature, adding a chitosanase solution, stirring and crosslinking at a low temperature to obtain a sodium alginate hydrosol for immobilizing chitosanase, and carrying out ultrasonic activating treatment to obtain activated immobilized chitosanase; and adding pretreated chitosan in an ultra-high-pressure reaction kettle, adding the activated immobilized chitosanase, feeding carbon dioxide gas, pressurizing and heating to enable carbon dioxide to be in a supercritical state, keeping heating to an enzymolysis temperature, and carrying out enzymolysis to obtain the chitosan oligosaccharide. Micron chitosan powder and the chitosanase which is crosslinked andimmobilized to a sodium alginate hydrosol system are taken as raw materials, and with the aid of the supersolubility of the supercritical carbon dioxide fluid, the chitosan oligosaccharide which is simple and convenient to operate, high in efficiency and narrow in molecular weight is prepared.
Owner:ZHEJIANG OCEAN UNIV

f175-3 protein and its coding gene and application of hydrolyzed cellulose

The invention discloses an F175-3 protein, a coding gene thereof and an application of the F175-3 protein to hydrolyzing cellulose. The invention provides the protein shown in the following 1) or 2): 1) a protein formed by amino acid residues shown in a sequence 2 in a sequence table; 2) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 2 in the sequence table, has the same functions as the protein shown in 1) and is derived from the protein shown in 1). Experiments prove that new cellulase F175-3 can still retain 88.5% of activity after being incubated at an optimum temperature of 60 DEG C for two hours under the conditions that cellulose is taken as the substrate and the pH value is 5.0; the new cellulase F175-3 can retain more than 80% of residual activity in a pH value range of 3.5-8.0 after being incubated in buffer solutions with different pH values for 24 hours at 4 DEG C.
Owner:PEKING UNIV

A kind of preparation method of duck reovirus egg yolk antibody

The invention discloses a preparation method of duck reovirus yolk antibody, which comprises the following steps: (1) using duck reovirus inactivated vaccine to immunize breeding-aged hens to obtain high-immunity eggs; (2) collecting high-immunity eggs Egg yolk, after removing the vitelline membrane and frenulum, crush it with a colloid mill to obtain egg yolk liquid; (3) mix the egg yolk liquid obtained in step (2) with purified water at a volume ratio of 1:1 to 3, stir well and then add The final concentration weight volume ratio is 1-3% citric acid and 1-4% polyanion cellulose, after fully stirring, place it at low temperature for 3-6 hours, centrifuge at 1-5 ° C, and collect the supernatant; (4) the step (3) The collected supernatant was sterilized by filtration to obtain egg yolk antibody liquid. The preparation method of duck reovirus yolk antibody disclosed by the invention has simple steps, high efficiency and yield, less addition, and is economical and applicable.
Owner:广东渔跃生物技术有限公司

A process for separating albumin from hemolyzed serum

The invention discloses a process for separating albumin from hemolyzed serum, which is characterized in that it comprises the following steps: (1) adding activated carbon to the hemolyzed serum, freezing, and then standing at normal temperature until the serum is completely frozen-thawed and stratified, Extract the upper layer solution, filter and collect filtrate A; (2) Gel chromatography the filtrate A, collect the eluent to obtain solution B; (3) add carboxylated ferric oxide nano-magnetic beads to solution B, and after incubation at room temperature, Filtration and separation to obtain solution C; (4) ultrafiltration and concentration of solution C to obtain protein concentrate D; (5) virus inactivation and drying of protein concentrate D to obtain albumin powder. The raw material cost of the method is low, and the prepared albumin has good purity and high safety.
Owner:GUANGZHOU RUITE BIOTECH CO LTD

A kind of durian yogurt pudding and preparation method thereof

The invention discloses a durian yogurt pudding and a preparation method thereof. The composition per 100 parts by weight includes: 60-80 parts of milk, 1-5 parts of whole milk powder, 0.1-2 parts of durian pulp, and 1-6 parts of cream 0.5-1.2 parts of sweetener, 0.5-1.2 parts of compound emulsifying thickener, 0.05-0.15 parts of essence, 0.02-0.03 parts of mixed bacteria powder of Streptococcus thermophilus and Lactobacillus bulgaricus, and the balance of water. The durian yogurt pudding has the advantages of high nutritional value, fine and smooth taste, uniform and stable, and long shelf life. The preparation method simplifies the technological process, is suitable for industrial production, and ensures the vitality of bacteria to the greatest extent, enhances the nutritional and functional characteristics of the product, and is more beneficial to the health of the human intestinal tract.
Owner:HEBEI BROS ILONG FOOD TECH LLC

A kind of cartilage tissue cryopreservation solution and its application

The invention discloses a cartilage tissue low-temperature refrigeration preservation solution and application thereof. The preservation solution comprises one or more of a DMEM medium, 1-30mM / L HEPES, a 1-20mM / mL component A, a 10-100ng / mL transforming growth factor beta, a 10-100ng / mL component B, 10-100g / L chondroitin sulfate and a 100-1000microg / mL matrix metalloproteinase inhibitor, wherein the component A is bongkrekic acid or Q-VD-OPH; and the component B is cartilage-derived morphogenetic protein 1, BMP or PDGF. The preservation solution provided by the invention can prolong the preservation time of fresh cartilage, and after preservation for 8 weeks, the cartilage still meets the requirements of clinical transplantation for activity and functionality.
Owner:江苏科瑞百奥生物技术有限公司

A method for the controlled cultivation of cell density and arrangement based on insect wing substrate

The invention discloses a controllable culture method of cell density and arrangement based on an insect wing substrate. The method uses insect scale wings as a base, and the insect scale wings are placed obliquely in a cell suspension, and the cells fall vertically under the action of gravity. On the surface of the insect scales, the number of cells gradually increases from top to bottom; the cells on the surface of the insect scales are oriented according to the micro-nano structure on the surface of the insect scales. The invention uses insect scales as the substrate, which is easy to obtain, safe and non-toxic, and can realize cell culture with controllable cell density and arrangement at the same time, and avoids a series of complex processes and safety problems caused by artificial synthetic materials; it uses gravity to form The density gradient distribution avoids the damage of mechanical force to cells and retains cell viability. The formed cell arrangement with directional arrangement and density gradient distribution has potential application prospects and reality for tissue regeneration, including regeneration and repair of connective tissue. significance.
Owner:SOUTHEAST UNIV

f85-20 protein and its coding gene and its application as β-glucosidase

The invention discloses an F85-20 protein, a coding gene thereof and an application of the F85-20 protein as beta-glucosidase. The invention provides the protein shown in the following 1) or 2): 1) a protein formed by amino acid residues shown in a sequence 2 in a sequence table; 2) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 2 in the sequence table, has the same functions as the protein shown in 1) and is derived from the protein shown in 1). Experiments prove that new beta-glucosidase F85-20 can still retain 80% of activity after being incubated at 50 DEG C for two hours under the conditions that p-nitrophenol glucoside (pNPG) is taken as the substrate and the pH value is 6.0, and the optimum temperature is 65 DEG C; new beta-glucosidase F85-20 can retain more than 70% of residual activity in a pH value range of 4.0-7.5 after being incubated in buffer solutions with different pH values for 24 hours at 4 DEG C.
Owner:PEKING UNIV

An enzyme-free one-pot colorimetric detection method for glucose based on enzyme-like nanomaterials

The invention relates to the field of nanomaterials, catalysis and analytical chemistry, in particular to a non-enzymatic one-pot glucose chromogenic detection method based on biomimetic nanomaterials. The method comprises the steps that bovine serum albumin is mixed with manganous ions by means of nanostructure characteristics of the bovine serum albumin and properties of functional groups of the bovine serum albumin, after sodium hydroxide is added, a normal temperature reaction is conducted, and then manganese dioxide nanosheets are generated under the stability of the bovine serum albumin. The material has the dual activities of imitation peroxidase and imitation glucose oxidase, and the optimal activity conditions of the two biomimetic activities are similar. Therefore, a novel one-step non-enzymatic glucose chromogenic detection method is established, and the material has a wide application prospect in the fields of analytical chemistry, environmental engineering and catalysis.
Owner:QINGDAO AGRI UNIV

A kind of ice crystal-free cryopreservation solution and freezing method for cartilage, tendon and meniscus preservation

ActiveCN111657267BAvoid regrowthIncreased number of living cellsDead animal preservationMeniscal tissueIce crystals
The invention discloses an ice crystal-free cryopreservation solution and a freezing method for cartilage, tendon and meniscus preservation. 0.1-2.5mol / L of piperazine ethylsulfonic acid, Caspase inhibitor Z-VAD-FMK 10-50μmol / L, matrix metalloproteinase inhibitor GM6001 and component X of 2-10mg / L. The preservation solution disclosed by the present invention can effectively increase the number of living cells and cell viability in cartilage, tendon, and meniscus tissue after cryopreservation, and more importantly, it can prevent ice crystals from growing again during freezing recovery and during storage. vitrified. This new type of ice crystal-free cryoprotectant allows cartilage, tendon, and meniscus to be stored for a longer period of time, and can be stored permanently at an environment of less than ‑130 °C.
Owner:江苏科瑞百奥生物技术有限公司

Method for producing laundry detergent

The invention relates to the field of cleaning chemical products, and provides a method for producing a laundry detergent. The method comprises the following steps of: firstly, pouring sodium lauryl sulfate, sodium citrate, sodium dodecylbenzene sulfonated and sodium perborate into a blending boiler according to proportions, and stirring until the solution is uniformly dispersed; then adding fatty alcohol-polyoxyethylene ether, menthyl acetate and mature vinegar, stirring uniformly, and then adding laccase; then adding tea tree oil and a chamomile extract in sequence; finally, refluxing and then detecting. The method is simple and can better keep the activity of the components of the laundry detergent, so that the washing effect of a product can be beneficially improved. Besides, the method has low requirements for equipment and can be popularized and applied more easily.
Owner:王振星

Durian yogurt pudding

The invention discloses a durian yoghourt pudding. Each 100 weight parts of the durian yoghourt pudding includes: 60 to 80 parts of milk, 1 to 5 parts of whole milk powder, 0.1 to 2 parts of durian pulp, 1 to 6 parts of dilute cream, 0.5 to 1.2 parts of a sweetener, 0.5 to 1.2 parts of a compound emulsified thickener, 0.05 to 0.15 parts of a essence, 0.02 to 0.03 parts of Streptococcus thermophilus and Lactobacillus bulgaricus mixed powder, and the balance of water. The durian yoghurt pudding has the advantages of high nutritional value, delicate taste, smoothness, uniform stability and long shelf life. A making method simplifies the process flow, is suitable for industrial production, ensures the vitality of the strain to the greatest extent, enhances the nutritional and functional characteristics of a product, and is more beneficial to human intestinal health.
Owner:孙晓菲

A kind of preparation method of direct throw type chymosin preparation and its product and application

The invention discloses a preparation method of a directed vat set chymosin preparation and a product and an application thereof. The preparation method comprises the following steps: culturing paenibacillus (Paenibacillus sp.) at 25-35 DEG C by using a skimmed milk culture medium, shake culturing for 24-120 hours to obtain fermentation broth, centrifuging the fermentation broth and extracting the supernatant to obtain the directed vat set chymosin preparation. According to the preparation method, the skimmed milk is innovatively used as a base material for fermentation, since being a milk product as well, the obtained chymosin can be directly applied to the preparation process of cheese, compared with the traditional chymosin preparation method, the method is used for greatly simplifying the application steps of the chymosin preparation, retaining the chymosin activity to the uttermost and completely avoiding the enzyme activity loss of chymosin in the extraction process, so as to effectively reduce the production and application costs of the chymosin preparation.
Owner:BRIGHT DAIRY & FOOD CO LTD

Application of f52-2 protein and its coding gene and hydrolyzed xylan

The invention discloses F52-2 protein and application of a coding gene of the F52-2 protein and hydrolyzed xylan. The protein disclosed by the invention is protein (1) or (2), wherein the protein (1) is formed by amino acid residues shown in a sequence 2 in a sequence table, and the protein (2) is formed through enabling a residue sequence of an amino acid sequence of the sequence 2 in the sequence table to be subjected to the substitution and / or deletion and / or addition of one or more amino acid residues and is protein which has the same functions and is derived from the protein (1). According to the F52-2 protein and the application of the coding gene of the F52-2 protein and the hydrolyzed xylan, proved by experiments, the optimal temperature for novel beta-xylosidase F52-2 is 60 DEG C under the conditions of taking p-nitrophenol xyloside (pNPX) as a substrate and being in the pH value of 5.0, and activity of 85% can still be maintained after the novel beta-xylosidase F52-2 is incubated for 2 hours at the temperature of 50 DEG C; over 60% residual activity can be maintained in the pH value of 3.5-5.5 after the novel beta-xylosidase F52-2 is incubated for 24 hours in buffer solutions with different pH values at the temperature of 4 DEG C.
Owner:PEKING UNIV

F52-2 protein and application of coding gene of F52-2 protein and hydrolyzed xylan

The invention discloses F52-2 protein and application of a coding gene of the F52-2 protein and hydrolyzed xylan. The protein disclosed by the invention is protein (1) or (2), wherein the protein (1) is formed by amino acid residues shown in a sequence 2 in a sequence table, and the protein (2) is formed through enabling a residue sequence of an amino acid sequence of the sequence 2 in the sequence table to be subjected to the substitution and / or deletion and / or addition of one or more amino acid residues and is protein which has the same functions and is derived from the protein (1). According to the F52-2 protein and the application of the coding gene of the F52-2 protein and the hydrolyzed xylan, proved by experiments, the optimal temperature for novel beta-xylosidase F52-2 is 60 DEG C under the conditions of taking p-nitrophenol xyloside (pNPX) as a substrate and being in the pH value of 5.0, and activity of 85% can still be maintained after the novel beta-xylosidase F52-2 is incubated for 2 hours at the temperature of 50 DEG C; over 60% residual activity can be maintained in the pH value of 3.5-5.5 after the novel beta-xylosidase F52-2 is incubated for 24 hours in buffer solutions with different pH values at the temperature of 4 DEG C.
Owner:PEKING UNIV

A kind of preparation method of egg yolk antibody of Pasteurella avian capsule antigen group A

The invention discloses a preparation method of a pastevula mulfocida capsular antigen A group yolk antibody. The method includes the following steps that (1) a pastevula mulfocida capsular antigen Agroup inactivated vaccine is used for immunizing reproductive-age hens to obtain high-immune eggs; (2) high-immune egg yolks are collected, yolk membranes are removed, and crushing is carried out to obtain yolk liquid; (3) the yolk liquid obtained in step (2) and purified water are mixed in a volume ratio of 1:(2-4) and stirred to be uniform, L-ascorbic acid with a final concentration of 1-3% (m / v), propylene glycol with a final concentration of 4-8% (m / v) and dextran sulfate with a final concentration of 3-6% (m / v) are added, after sufficient stirring, the mixture is placed for 2-4 h at a lowtemperature, low-temperature centrifugation is carried out, and supernate is collected; (4) the supernate collected in step (3) is subjected to filtration sterilization to obtain yolk antibody liquid. The preparation method of the pastevula mulfocida capsular antigen A group yolk antibody includes simple steps, has high efficiency and yield and is economical and applicable.
Owner:广东渔跃生物技术有限公司

Probiotic compound for treating inflammatory gynecological diseases

PendingCN113274415AGrowth inhibitionTo achieve the effect of treating inflammatory gynecological diseasesAntibacterial agentsAntimycoticsBiotechnologyFreeze-drying
The invention provides a probiotic compound for treating inflammatory gynecological diseases, and belongs to the field of microbial preparations. The probiotic compound comprises a composite culture medium, viable probiotics and a carrier, wherein the viable probiotics comprise lactobacillus and / or bifidobacterium. A preparation method comprises the following steps: activating the lactobacillus and / or the bifidobacterium by using a first culture medium, performing enlarged culture, inoculating the cultured strain into a second culture medium, performing fermentation culture to obtain fermentation broth, adding a freeze-drying protective agent and a carrier, and performing freeze-drying to obtain the lactobacillus and / or bifidobacterium freeze-dried powder. The second culture medium comprises the first culture medium and soybean polypeptide powder. According to the invention, the vitality of the strain is reserved to the greatest extent through early-stage culture and direct freeze-drying, and besides, a plurality of active substances are introduced on the carrier, so that the carrier can quickly stimulate an immune system to maintain micro-ecological balance, the medication time is shortened, and the medication efficiency is improved.
Owner:镇江远森医药科技有限公司
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