Production process for tobacco bacterial wilt resistant compound microbial agent

A compound microbial inoculant, a technology for tobacco bacterial wilt, applied in the directions of microorganism-based methods, microorganisms, and chemicals for biological control, etc., can solve problems such as the inability to achieve long-term disease resistance, and enhance plant stress resistance. , Conducive to the effect of amplifying production and the production process is simple and easy to implement

Inactive Publication Date: 2017-12-08
烟台丰成农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to compounding the antagonistic bacteria after fermentation, the above patents do not add chitosan oligosaccharides, a beneficial ingredient that can improve tobacco resistance, and without production treatments such as sticking and coating, the effect of long-term disease resistance cannot be achieved. The present invention can solve this problem well

Method used

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  • Production process for tobacco bacterial wilt resistant compound microbial agent
  • Production process for tobacco bacterial wilt resistant compound microbial agent
  • Production process for tobacco bacterial wilt resistant compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a) Preparation of Bacillus colloidus cells: the strains were purchased from Beina Biology, strain number BNCC173119, 50 kg of culture medium was prepared, and the formula was 0.5 kg of peptone, 0.15 kg of beef extract, 0.25 kg of sodium chloride, pH 7.5, 121 Sterilize at ℃ for 30 minutes, insert the purchased strains, and culture on a shaking table at 150r / min for 24 hours to obtain the strains; prepare 1t of expansion medium, the formula is 40kg of beef extract, 9kg of yeast extract powder, 3kg of sodium chloride, pH7.5 , transferred to a fermenter for high-temperature sterilization, and after the sterilization, when the temperature dropped to 36°C, 50kg of the seed liquid was inserted, and the rotation speed was 120r / min for 26h, and the fermentation liquid was transferred to 4000r / min and centrifuged for 30min to obtain Bacillus colloidus Bacteria 128.7kg.

[0024] b) Preparation of Trichoderma harzianum thallus: purchased from Trichoderma Strain Collection Managemen...

experiment example 1

[0031] Experimental example 1: Tobacco bacterial wilt potted plant disease control test

[0032] Use the compound microbial inoculum produced by the method of embodiment 1 to carry out potted plant disease prevention test, test procedure is as follows:

[0033] The experimental tobacco variety was purchased locally as "China Tobacco 100". The bacterial wilt pathogen, R. solanacearum, was purchased from Beina Biological No. BNCC221120. The experimental soil was taken from the field of Youyi Village, Laishan Town, Yantai City, which is prone to tobacco bacterial wilt. Soil, texture sandy loam, pH=5.3, organic matter 7.3g / kg, total nitrogen (N) 0.762g / kg, total phosphorus (P 2 o 5 )0.577g / kg, total potassium (K 2 O) 15.3g / kg. Three treatments were set up in the test, which are: T1: root irrigation with Jinzhengda antimicrobial agent, the application rate is 3ml / m 2 ; T2: use the bacterial agent of the present invention to spread, and the application rate is 5g / m 2 ; T3: No b...

experiment example 2

[0038] Experimental example 2: Field experiment on tobacco bacterial wilt control

[0039] Use the compound microbial bacterial agent produced by the method of embodiment 1 to carry out field test, from April 2016 to September 2016, carry out field test in Xiaohezi Village, Fushan District, Yantai City, and the experimental field for testing is the incidence rate of bacterial wilt disease in recent years. The more serious plots were spread with organic fertilizers, and the experimental tobacco variety was NC89. The test set 2 treatments, respectively: T1: use the bacterial agent of the present invention to spread, and the application rate is 5kg / 667m 2 ; T2: No bacterial agent was used as the control group. Each treatment was repeated 4 times, and the area of ​​the plot was 100m 2 .

[0040] Observe the disease on 30d, 60d, 90d, and 120d after planting. Disease grade: 0 is asymptomatic on the leaves; 1 is wilting on the leaves below 1 / 4 of the plant; The surface of the pla...

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Abstract

The invention provides a production process for a tobacco bacterial wilt resistant compound microbial agent. Fertilizer comprises the raw materials of 15-20 parts of bacillus mucilaginosus, 15-20 parts of trichoderma harzianum, 15-20 parts of streptomyces microflavus, 10-35 parts of chitosan oligosaccharide, 10-15 parts of vegetable oil sludge and 10-15 parts of plant ash. By adopting the production process of bacterium preparation, dosing, compound bacterium pelletizing, first-layer vegetable oil sludge adhesive-smearing, second-time plant ash adhesive-smearing and third-time chitosan oligosaccharide adhesive-smearing, the three probiotics are combined together and meanwhile separated from the antibacterial component, namely, the chitosan oligosaccharide, so that the respective effects are given to play to the maximum extent. Through the antagonism effect and the killing effect of microorganisms on bacterial wilt bacteria, and induced resistance of the chitosan oligosaccharide to tobacco, tobacco bacterial wilt is prevented and treated; and the advantages of non-pollution, environmental friendliness, low cost and lasting effect are achieved.

Description

technical field [0001] The invention belongs to the technical field of fertilizer processing, and in particular relates to a production process of a compound microbial agent for resisting tobacco bacterial wilt. Background technique [0002] Tobacco is an important agricultural economic crop in our country and occupies a relatively important position in the agricultural economy, especially in some places where the economic development is relatively lagging behind. It is a basic means to meet people's basic living requirements. Tobacco bacterial wilt is caused by Ralstonia solanacearum, and its host range includes more than 200 kinds of crops, among which potato, tomato, tobacco and other Solanaceae crops suffer the most serious damage. Now, plant bacterial wilt has become a worldwide phenomenon. It is one of the major diseases that spread widely and cause serious harm in China. At present, the control methods for tobacco bacterial wilt are mainly to improve the resistance o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/04A01N63/00A01N43/16A01P1/00A01P21/00C12R1/07C12R1/885C12R1/465
CPCA01N43/16A01N63/00A01N63/30C12N1/20A01N2300/00
Inventor 熊廷珍
Owner 烟台丰成农业科技有限公司
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