Production process for potato bacterial wilt resistant compound microbial agent

A compound microbial agent, potato bacterial wilt technology, applied in the direction of microorganism-based methods, microorganisms, biocides, etc., can solve the problems of inability to achieve long-term disease resistance, ineffective effect, waste of traditional Chinese medicine resources, etc., and achieve enhanced The effect of plant stress resistance, simple and easy production process, and high survival rate

Inactive Publication Date: 2017-12-08
烟台丰成农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research of preventing and treating potato bacterial wilt has a certain history, such as patent application No. 201010608565.X, an antagonistic bacteria and microbial organic fertilizer that can control potato green potato bacterial wilt, adopts the independent antagonistic bacteria (BacillussubtilisI-23) to add To organic fertilizers, and add nitrogen, phosphorus and potassium nutrients to make bio-organic fertilizers to prevent potato bacterial wilt; another example is the traditional Chinese medicine composition disclosed in Application No. 201310580772.2, a method of using disease-resistant drugs for potato bacterial wilt. The anti-disease drugs used in this field have caused a certain degree of waste of traditional Chinese medicine resources; another example is the application number 201510132194.5, a disease-resistant drug for potato bacterial wilt, which discloses a disease-resistant formula using licorice, tangerine peel, pepper and formalin. The method is simple, but the effect is not obvious
In addition to adding antagonistic bacteria to fertilizers, the above patents do not add chitosan oligosaccharides, a beneficial ingredient that can improve potato resistance, and without production treatments such as sticking and coating, they cannot achieve long-term disease resistance. Invention can solve this problem well

Method used

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  • Production process for potato bacterial wilt resistant compound microbial agent
  • Production process for potato bacterial wilt resistant compound microbial agent
  • Production process for potato bacterial wilt resistant compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a) Preparation of Bacillus colloidus cells: the strains were purchased from Beina Biology, strain number BNCC173119, 50 kg of culture medium was prepared, and the formula was 0.5 kg of peptone, 0.15 kg of beef extract, 0.25 kg of sodium chloride, pH 7.5, 121 Sterilize at ℃ for 30 minutes, insert the purchased strains, and culture on a shaking table at 150r / min for 24 hours to obtain the strains; prepare 1t of expansion medium, the formula is 40kg of beef extract, 9kg of yeast extract powder, 3kg of sodium chloride, pH7.5 , transferred to a fermenter for high-temperature sterilization, and after the sterilization, when the temperature dropped to 36°C, 50kg of the seed liquid was inserted, and the rotation speed was 120r / min for 26h, and the fermentation liquid was transferred to 4000r / min and centrifuged for 30min to obtain Bacillus colloidus Bacteria 128.7kg.

[0026] b) Preparation of Trichoderma harzianum thallus: purchased from Trichoderma Strain Collection Managemen...

experiment example 1

[0033] Experimental example 1: Potted plant disease control experiment of potato bacterial wilt

[0034] Use the compound microbial inoculum produced by the method of embodiment 1 to carry out potted plant disease prevention test, test procedure is as follows:

[0035] The experimental potato variety "Holland No. 7" was purchased locally. The bacterial wilt pathogen, R. solanacearum, was purchased from Beina Biological No. BNCC221120. The experimental soil was taken from the field of Youyi Village, Laishan Town, Yantai City, which is prone to potato bacterial wilt. Soil, texture sandy loam, pH=5.3, organic matter 7.3g / kg, total nitrogen (N) 0.762g / kg, total phosphorus (P 2 o 5 )0.577g / kg, total potassium (K 2 O) 15.3g / kg. A total of 3 treatments were set up in the test, which are: T1: root irrigation with Jinzhengda antimicrobial agent, the application rate is 3ml / m 2 ; T2: use the bacterial agent of the present invention to spread, and the application rate is 5g / m 2 ; T3...

experiment example 2

[0040] Experimental example 2: Field experiment on the control of potato bacterial wilt

[0041] Use the compound microbial bacterial agent produced by the method of embodiment 1 to carry out field test, from April 2016 to September 2016, carry out field test in Xiaohezi Village, Fushan District, Yantai City, and the experimental field for testing is the incidence rate of bacterial wilt disease in recent years. The more serious plots were spread with organic fertilizers, and the experimental potato variety was Yanshu No.4. The test set 2 treatments, respectively: T1: use the bacterial agent of the present invention to spread, and the application rate is 5kg / 667m 2 ; T2: No bacterial agent was used as the control group. Each treatment was repeated 4 times, and the area of ​​the plot was 100m 2 .

[0042] Observe the disease on 30d, 60d, 90d, and 120d after planting. Disease grade: 0 is asymptomatic on the leaves; 1 is wilting on the leaves below 1 / 4 of the plant; The surfac...

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Abstract

The invention provides a production process for a potato bacterial wilt resistant compound microbial agent. Fertilizer comprises the raw materials of 15-20 parts of bacillus mucilaginosus, 15-20 parts of trichoderma harzianum, 15-20 parts of streptomyces microflavus, 10-35 parts of chitosan oligosaccharide, 10-15 parts of vegetable oil sludge and 10-15 parts of plant ash. By adopting the production process of bacterium preparation, dosing, compound bacterium pelletizing, first-layer vegetable oil sludge adhesive-smearing, second-time plant ash adhesive-smearing and third-time chitosan oligosaccharide adhesive-smearing, the three probiotics are combined together and meanwhile separated from the antibacterial component, namely, the chitosan oligosaccharide, so that the respective effects are given to play to the maximum extent. Through the antagonism effect and the killing effect of microorganisms on bacterial wilt bacteria, and induced resistance of the chitosan oligosaccharide to potatoes, potato bacterial wilt is prevented and treated; and the advantages of non-pollution, environmental friendliness, low cost and lasting effect are achieved.

Description

technical field [0001] The invention belongs to the technical field of fertilizer processing, and in particular relates to a production process of a compound microbial agent for resisting potato bacterial wilt. Background technique [0002] Potato, potato (scientific name: Solanum tuberosum L.), belongs to the perennial herbaceous plant of Solanaceae, and its tubers are edible. It is the fourth most important food crop in the world, second only to wheat, rice and corn. Potatoes are also called ground eggs, potatoes, potatos, etc., the tubers of Solanaceae plants. Together with wheat, rice, corn, and sorghum, it has become the world's top five crops, with a large amount of daily planting. Potato bacterial wilt is caused by bacteria and is a bacterial vascular tissue disease. The leaves show that the new leaves on the top are initially wilting and drooping, and then the lower leaves develop and wither, and then the middle leaves wither. At the initial stage of the disease, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P3/00C12R1/07C12R1/885C12R1/465
CPCA01N63/00A01N63/30C12N1/14C12N1/20
Inventor 熊廷珍
Owner 烟台丰成农业科技有限公司
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