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Fungal-laccase expression strain and establishing method and application thereof

A fungal laccase and bacterial strain technology, applied in the field of fungal laccase expression strains and its construction, can solve the problems of difficulty in meeting industrial requirements, high cost, and low yield

Active Publication Date: 2019-04-23
ANHUI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some fungal laccase genes have been successfully recombinantly expressed, the heterologous expression of fungal laccase has the disadvantages of low yield, long fermentation cycle and high cost, and it is difficult to meet the requirements of industrialization.

Method used

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  • Fungal-laccase expression strain and establishing method and application thereof
  • Fungal-laccase expression strain and establishing method and application thereof
  • Fungal-laccase expression strain and establishing method and application thereof

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Embodiment Construction

[0075] The present invention transforms Trameteshirsuta AH28-2, a laccase high-yielding bacterial strain screened and isolated by our laboratory by genetic means, that is, the heat shock protein ThHspA1 is overexpressed in the bacterial strain, and bacterial strain Trameteshirsuta AH28-2 (ThHspA1 -5) The production of laccase can be increased by 2 times under the condition of adding 1 / 10 of the initial dose of inducer, and the production of laccase with this strain has the advantages of high efficiency and environmental protection.

[0076] The classification of Trametes sp.AH28-2 is named: Trametes sp.AH28-2, deposit unit: China Collection Center for Typical Cultures, address: China. Wuhan. Wuhan University, deposit date: November 29, 2005, deposit number : CCTCC NO: M205134.

[0077] Trametes hirsuta AH28-2 (ThHspA1-5), a fungal laccase expression strain of the present invention, depository unit: China Typical Culture Collection Center, address: China. Wuhan. Wuhan Universit...

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Abstract

The invention discloses a fungal-laccase expression strain and an establishing method and application thereof. A laccase high-producing strain-trametes AH28-2 is modified with a genetic mean, namely,heat shock protein ThHspA1 is subjected to overexpression in the strain, and through the modified strain Trametes hirsuta AH28-2 (ThHspA1-5), the laccase yield can be increased by 2 times under the condition that 1 / 10 initial dose of an inductive agent is added, and therefore laccase produced through the strain has the advantages of being efficient, environmentally friendly and the like.

Description

technical field [0001] The invention relates to a fungal laccase expression strain, a construction method and application thereof. Background technique [0002] Fungal laccase (laccase, EC1.10.3.2) is a class of copper-containing polyphenol oxidase, which is widely distributed in higher filamentous fungi, especially basidiomycetes, and can catalyze the oxidation of hundreds of phenolic and nonphenolic compounds. , while reducing oxygen molecules to water; in the presence of small molecule mediator substances such as methyl syringate, the substrates of fungal laccase can be further broadened. In recent years, with the society's appeal and pursuit of sustainable and environmentally friendly catalysis, fungal laccases have a wide substrate spectrum, and O 2 as the electron acceptor by-product is H 2 The catalytic advantages of O and other aspects are gradually reflected, and the research on fungal laccases has therefore attracted extensive attention from scientific researcher...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/80C12N9/02C12R1/645
CPCC12N9/0061C12N15/80C12Y110/03002C12R2001/645C12N1/145
Inventor 方泽民赵文梦张艳凤王晶晶肖亚中
Owner ANHUI UNIVERSITY
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