A method for determining 12 kinds of polyphenolic compounds in fresh tobacco leaves

A technology of polyphenolic compounds and polyphenolic compounds, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unstable detection and difficulty in obtaining standard substances, etc., and achieve the effect of good precision and strong solvent effect

Active Publication Date: 2020-08-14
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the wide variety of plant polyphenols, some are not easy to obtain standard substances, and some cannot be stably detected in tobacco

Method used

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  • A method for determining 12 kinds of polyphenolic compounds in fresh tobacco leaves
  • A method for determining 12 kinds of polyphenolic compounds in fresh tobacco leaves
  • A method for determining 12 kinds of polyphenolic compounds in fresh tobacco leaves

Examples

Experimental program
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Embodiment 1

[0042] The method for measuring 12 kinds of polyphenolic compounds in the fresh tobacco leaf of the present embodiment comprises the steps:

[0043] Step (1), preparing standard solutions of 12 kinds of polyphenol standard substances

[0044] Weigh 0.1 g of 12 polyphenol standard substances into 100 mL volumetric flasks, accurate to 0.0001 g, and dilute to volume with methanol to prepare a single standard stock solution with a concentration of 1000 μg / mL.

[0045] Pipette 1 mL each of the 4 single standard stock solutions of caffeic acid, ferulic acid, scopoletin and coumarin into a 20 mL volumetric flask, and dilute to volume with 50% volume concentration of methanol to obtain 4 with a concentration of 50 μg / mL. A mixed stock solution low in polyphenols.

[0046] Pipette 5 mL each of the five single standard stock solutions of neochlorogenic acid, hyoscyamine, kaempferol, kaempferol, and kaempferol-3-O-rutinoside into a 50 mL volumetric flask, and dilute to volume with 50% v...

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Abstract

The invention relates to a method for determining 12 polyphenol compounds in a fresh tobacco leaf. The method comprises the following steps: preparation of standard solutions of 12 polyphenol standardsubstances, sample treatment and sample UPLC-MS-MS analysis and calculation. For the 12 polyphenols determined in the method, recovery rate is 84.7%-102%; relative standard deviation is 1.9%-5.7%; and limit of quantitation is 0.5-8.0 mcg / g. The method is sensitive and accurate, and is suitable for content analysis of 12 polyphenols in the fresh tobacco leaf.

Description

technical field [0001] The invention relates to a method for determining polyphenol compounds, in particular to a method for determining 12 kinds of polyphenol compounds in fresh tobacco leaves. Background technique [0002] Polyphenolic compounds are a class of substances that are abundant in tobacco, and play an important role in the growth and development of tobacco, the appearance and color of tobacco leaves, modulation characteristics, flavor and taste, and the physiological strength of smoke. It is a measure of tobacco quality. Key factor. The content and oxidation degree of tobacco polyphenols not only affect the appearance quality of tobacco leaves, but also affect the inner quality of tobacco leaves. Polyphenols produce slightly acidic products when tobacco is smoked, which can neutralize part of the alkalinity, alcohol and smoke of the smoke. Some polyphenols themselves have a pleasant aroma, and they are cracked to produce a variety of aromatic substances during...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96
CPCG01N30/96
Inventor 宋春满邓乐乐向海英高茜曾婉俐李雪梅陈章玉
Owner CHINA TOBACCO YUNNAN IND
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