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Vaccine adjuvant, application thereof and porcine reproductive and respiratory syndrome vaccine

A respiratory syndrome and vaccine adjuvant technology, which is applied in the field of vaccine adjuvants and porcine reproductive and respiratory syndrome vaccines, can solve the problems of weak cellular immune response and unsatisfactory immune protection effect, so as to reduce viremia and maintain time Longer, less immunosuppressive effects

Pending Publication Date: 2019-05-14
龙阔(苏州)生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the inactivated PRRS vaccine is safe, it only induces a very weak cellular immune response, and its immune protection effect is not ideal

Method used

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  • Vaccine adjuvant, application thereof and porcine reproductive and respiratory syndrome vaccine
  • Vaccine adjuvant, application thereof and porcine reproductive and respiratory syndrome vaccine
  • Vaccine adjuvant, application thereof and porcine reproductive and respiratory syndrome vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A vaccine adjuvant, adding small molecular peptides, diethylaminoethyl glucose and stabilizers to deionized water in sequence, stirring at the maximum speed without foaming, and then adding aluminum hydroxide gel after mixing, the obtained water-soluble Sexual suspension (volume 2mL), the concentration of small molecular peptide in this water-soluble suspension is 1.5mg / mL, the concentration of diethylaminoethylglucose is 1.5g / mL, the concentration of stabilizer is 1g / mL ; The mass fraction of aluminum hydroxide glue accounting for the water-soluble suspension is 10%.

[0031] The pH value of the vaccine adjuvant in this example is 6.8.

[0032] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

Embodiment 2

[0034] A vaccine adjuvant, adding small molecular peptides, diethylaminoethyl glucose and stabilizers to deionized water in sequence, stirring at the maximum speed without foaming, and then adding aluminum hydroxide gel after mixing, the obtained water-soluble Sexual suspension (volume is 2mL), the concentration of the small molecular peptide in this water-soluble suspension is 0.5mg / mL, the concentration of diethylaminoethylglucose is 2.5g / mL, the concentration of stabilizer is 0.5g / mL mL; aluminum hydroxide gel accounts for 10% by mass of the water-soluble suspension.

[0035] The pH value of the vaccine adjuvant in this example is 7.8.

[0036] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

Embodiment 3

[0038] A vaccine adjuvant, adding small molecular peptides, diethylaminoethyl glucose and stabilizers to deionized water in sequence, stirring at the maximum speed without foaming, and then adding aluminum hydroxide gel after mixing, the obtained water-soluble Sexual suspension (volume is 2mL), the concentration of the small molecular peptide in this water-soluble suspension is 2.5mg / mL, the concentration of diethylaminoethylglucose is 1g / mL, the concentration of stabilizer is 1.5g / mL ; Aluminum hydroxide glue accounts for 20% in mass fractions of the water-soluble suspension.

[0039] The pH value of the vaccine adjuvant in this example is 7.8.

[0040] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

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Abstract

The invention is applicable to the field of biomedicine, and provides a vaccine adjuvant, application thereof and a porcine reproductive and respiratory syndrome vaccine. The vaccine adjuvant is a water-soluble suspension obtained by adding small molecule peptide, diethylaminoethyl glucose, aluminum hydroxide gel and a stabilizer to deionized water; the concentration of the small molecule peptidein the water-soluble suspension is 0.5-2.5 mg / mL, the concentration of diethylaminoethyl glucose is 1-2.5 g / mL, and the concentration of the stabilizer is 0.5-1.5 g / mL; the aluminum hydroxide gel accounts for 10-20% of the water-soluble suspension by mass. The vaccine adjuvant can induce the differentiation ratio of M1 and M2 in macrophages and kill different types of PRRSV strains; in combinationwith a porcine reproductive and respiratory syndrome virus (PRRSV) inactivating antigen, the cellular immunity of the body can be improved, interferon is induced, and the immunosuppression produced by the PRRSV is reduced. Thus, a high-content neutralizing antibody is quickly produced, the maintenance time is long, and the viremia of different virus strains can be reduced.

Description

technical field [0001] The invention belongs to the field of medicine and biology, and in particular relates to a vaccine adjuvant and its application and a porcine reproductive and respiratory syndrome vaccine. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS, Porcine Reproductive and Respiratory Syndrome) is a highly contagious disease in pigs. The pathogen is porcine reproductive and respiratory syndrome virus. The main host of the virus is domestic pigs and wild boars in nature. Pigs of different ages, breeds and genders can be infected with PRRSV (Porcine Reproductive and Respiratory Syndrome Virus), among which pregnant sows and suckling piglets are more seriously affected. In addition to infecting animals, boar semen has also been identified as a common source of PRRSV infection. [0003] The current common method of preventing and controlling PRRS is immunization and vaccination. The commonly used PRRS vaccines in the world mainly i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P31/14
Inventor 赖志高俊锋王杰龙进学韩相敏马晶晶吴碧清
Owner 龙阔(苏州)生物工程有限公司
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