Liquid phase nanometer colloidal gold colorimetric method for quickly detecting malignant tumor DNA (deoxyribonucleic acid)

A nano-colloid and malignant tumor technology, applied in the field of malignant tumor detection, can solve the problems of disordered proliferation of malignant tumor cells, malignant tumors, abnormal cell metabolism, etc., and achieve the effect of promoting the use

Inactive Publication Date: 2019-05-17
吉林省爱诺德生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The normal methylation of nucleic acid is a modification of the stable state of DNA. Under the action of methyltransferase, it can be inherited to the DNA of newborn offspring during the replication process. It is a normal epigenetic mechanism of genes. If the methylation of nucleic acid The abnormality of methylation indicates that the cell metabolism is also abnormal. The normal high methylation of DNA indicates that the gene at this moment remains silent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:

[0021] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;

[0022] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;

[0023] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;

[0024] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...

Embodiment 2

[0028] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:

[0029] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;

[0030] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;

[0031] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;

[0032] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...

Embodiment 3

[0036] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:

[0037] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;

[0038] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;

[0039] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;

[0040] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...

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Abstract

The invention discloses a liquid phase nanometer colloidal gold colorimetric method for quickly detecting malignant tumor DNA (deoxyribonucleic acid), and belongs to the technical field of malignant tumor detection methods. The method has the technical characteristics that a glass container used for an experiment is cleaned with detergent, after the glass container finishes being cleaned with thedetergent, the glass container is cleaned with distilled water, then, the glass container is dipped in silicified reagent overnight, the inner surface of a gold manufacture vessel is subjected to silicified processing, and the glass container is cleaned with distilled water for standby; 1%(w/v) chloroauric acid and 1%(w/v) sodium citrate solution are prepared, and a filter membrane of which the aperture is 0.2 micrometer is used for filtering; and one magnetic force stirrer is put in a 1000mL of round-bottom flask which is cleaned, and 1000mL of ultrapure water is added. By use of the method,according to the color change of nanometer colloidal gold solution, whether DNA methylation is normal or not can be represented so as to bring convenience for medical personnel in making an early diagnosis for the malignant tumor before clinical symptoms appear, and therefore, the method is favorable for promotion and use.

Description

technical field [0001] The invention relates to the technical field of malignant tumor detection methods, in particular to a liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA. Background technique [0002] Malignant tumors are diseases with high incidence and high mortality that seriously threaten human health. Early diagnosis and early treatment are the most effective ways to prevent and reduce the mortality of malignant tumors. Therefore, finding early markers of malignant tumors has become the focus of attention. Detection of DNA methyl Normalization or not has become one of the recognized effective detection methods. [0003] The normal methylation of nucleic acid is a modification of the stable state of DNA. Under the action of methyltransferase, it can be inherited to the DNA of newborn offspring during the replication process. It is a normal epigenetic mechanism of genes. If the methylation of nucleic acid The abnormality ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/53G01N33/543
Inventor 王志新侯淑霞许文革单亚明杨琨
Owner 吉林省爱诺德生物工程有限公司
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