A digital pcr image restoration method based on Fourier transform and its application

A technology of Fourier transform and image restoration, which is applied in the field of image processing, can solve the problem of uneven illumination of the lens imaging system, and achieve the effects of avoiding waste of time and resources, saving costs, and shortening the development cycle
CN109816609BActive Publication Date: 2021-12-21PILOT GENE TECH HANGZHOU CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PILOT GENE TECH HANGZHOU CO LTD
Publication Date
2021-12-21

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Abstract

The invention relates to the technical field of image processing, in particular to a Fourier transform-based digital PCR image restoration method and its application. The described digital PCR image restoration method based on Fourier transform comprises the following steps: S1. acquire the image to be processed; S2. preprocessing of the image; S3. design filter; S4. frequency domain image filtering; S5. calculation matrix correction Coefficient; S6. Output the corrected image. The digital PCR image restoration method based on Fourier transform disclosed by the present invention solves the problem of high pixels in the central area of ​​the picture and low pixels in the edges caused by inhomogeneous illumination, and is used for subsequent judgment of whether the droplet is positive or negative based on the strength of the fluorescent signal. Provide accurate data.
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Description

technical field

[0001] The invention relates to the technical field of image processing, in particular to a Fourier transform-based digital PCR image restoration method and its application. Background technique

[0002] Digital PCR technology means that DNA or RNA samples are diluted and divided into independent micro-reaction units, labeled with fluorescent probes labeled with specific gene fragments, and subjected to molecular template PCR amplification. After the amplification is completed, it is judged according to the strength of the fluorescent signal Positive or negative approach to microreaction units. Fluorescent signal intensity is detected with an optical imaging system. When the optical lens is imaging, the phenomenon of uneven illumination will occur, resulting in normal imaging in the center of the field of view and dark imaging at the edge of the field of view. This phenomenon is determined by the characteristics of the lens, that is, the central area of ​​t...

Claims

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