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A SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou 1" and its application

A technology of molecular markers and growth traits, which is applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of convenient operation, increasing economic benefits of breeding, and increasing meat content

Active Publication Date: 2022-03-08
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of large-scale hybrid seed production and breeding, there is a certain deformity rate and body shape differentiation in the seedlings. In order to screen out the hybrid yellow catfish "Huangyou No. Molecular markers, so as to provide technical support for the efficient breeding of this new species, there is an urgent need to develop SNP molecular markers related to the growth traits of the hybrid yellow catfish "Huangyou No. 1"

Method used

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  • A SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou 1" and its application
  • A SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou 1" and its application
  • A SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou 1" and its application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] a) Obtaining the population of hybrid yellow catfish "Huangyou No. 1";

[0035] b) DNA extraction from the caudal fin of the hybrid yellow catfish "Huangyou 1";

[0036] c) performing PCR amplification on the genomic DNA of the hybrid yellow catfish "Huangyou No. 1" based on the SNP primers;

[0037] d) Sequencing the PCR amplification product, and determining the genotype of the SNP based on the sequencing result;

[0038] e) Correlation analysis between the SNP genotype and the growth traits of the hybrid yellow catfish "Huangyou No. 1";

[0039] f) The SNP locus was applied to the selection of different body types of the hybrid yellow catfish "Huangyou 1".

[0040] The specific operation is as follows:

[0041] a) Obtaining the population of hybrid yellow catfish "Huangyou No. 1":

[0042] The fish used in the experiment of the present invention are all pond cultured fishes, which were taken from the Lukou Base of Nanjing Fishery Science Research Institute in Jia...

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Abstract

The invention discloses a SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou No. 1" with a high meat content and its application. The sequence of the SNP molecular marker is shown in SEQ ID NO:1 , the SNP molecular marker is located at the 118th position of the sequence shown in SEQ ID NO: 1, and the base is C or T. The invention also discloses a pair of primers, a kit and a detection method for detecting the molecular marker. Using this SNP site, the hybrid yellow catfish "Huangyou No. 1" with a larger body length / head length ratio can be effectively screened out. The SNP loci disclosed in the present invention are not restricted by the age and sex of the hybrid yellow catfish "Huangyou No. Improve the meat content of the hybrid yellow catfish "Huangyou No. 1", and significantly increase the breeding economic benefits of the hybrid yellow catfish "Huangyou No. 1". The method is accurate, reliable and easy to operate.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou No. 1" and its application. The hybrid yellow catfish "Huangyou No. 1" can be selected by using the SNP molecular marker with a body type with a high meat content ", which belongs to the field of genetics and breeding of yellow catfish. Background technique [0002] Molecular marker-assisted breeding uses molecular markers closely linked to target traits for indirect selection. It selects target traits at the molecular level. It has high selection intensity, is not affected by the environment, and has reliable results, which can accelerate the breeding process. As a new generation of molecular markers, single nucleotide polymorphism (SNP) has been recognized in the field of breeding. It has the advantages of high polymorphism, genetic stability and convenient detection, and has been widely used in the field of molecular breeding of animal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6858
Inventor 尹绍武武兆文张鑫宇王宏玉裴雪莹王涛
Owner NANJING NORMAL UNIVERSITY
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