SNP molecular marker related to growth traits of high-flesh-content hybrid pelteobagrus fulvidraco Huangyou #1 and application of SNP molecular marker

A technology of molecular markers and growth traits, applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of increasing economic benefits of breeding, increasing meat content, and convenient operation

Active Publication Date: 2019-05-31
NANJING NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of large-scale hybrid seed production and breeding, there is a certain deformity rate and body shape differentiation in the seedlings. In order to screen out the hybrid yellow catfish "Huangyou No. Molecular markers, so as to provide technical support for the efficient breeding of this new species, there is an urgent need to develop SNP molecular markers related to the growth traits of the hybrid yellow catfish "Huangyou No. 1"

Method used

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  • SNP molecular marker related to growth traits of high-flesh-content hybrid pelteobagrus fulvidraco Huangyou #1 and application of SNP molecular marker
  • SNP molecular marker related to growth traits of high-flesh-content hybrid pelteobagrus fulvidraco Huangyou #1 and application of SNP molecular marker
  • SNP molecular marker related to growth traits of high-flesh-content hybrid pelteobagrus fulvidraco Huangyou #1 and application of SNP molecular marker

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Experimental program
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Effect test

Embodiment

[0034] a) Obtaining the population of hybrid yellow catfish "Huangyou No. 1";

[0035] b) DNA extraction from the caudal fin of the hybrid yellow catfish "Huangyou 1";

[0036] c) performing PCR amplification on the genomic DNA of the hybrid yellow catfish "Huangyou No. 1" based on the SNP primers;

[0037] d) Sequencing the PCR amplification product, and determining the genotype of the SNP based on the sequencing result;

[0038] e) Correlation analysis between the SNP genotype and the growth traits of the hybrid yellow catfish "Huangyou No. 1";

[0039] f) The SNP locus was applied to the selection of different body types of the hybrid yellow catfish "Huangyou 1".

[0040] The specific operation is as follows:

[0041] a) Obtaining the population of hybrid yellow catfish "Huangyou No. 1":

[0042] The fish used in the experiment of the present invention are all pond cultured fishes, which were taken from the Lukou Base of Nanjing Fishery Science Research Institute in Jia...

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Abstract

The invention discloses an SNP molecular marker related to growth traits of high-flesh-content hybrid pelteobagrus fulvidraco Huangyou #1 during breeding and application of the SNP molecular marker. The sequence of the SNP molecular marker is shown in SEQ ID NO:1, the SNP molecular marker is located at the 118th site of the sequence shown in SEQ ID NO:1, and the basic group is C or T. The invention also discloses a primer pair, a kit and a detection method for detecting the molecular marker. By utilizing the SNP site, hybrid pelteobagrus fulvidraco Huangyou #1 with a larger ratio of the body length to the head length can be effectively screened out. The SNP site can be free of limitation of the age, sex and the like of the hybrid pelteobagrus fulvidraco Huangyou #1, and can be used for body form selection and classification of fry of the hybrid pelteobagrus fulvidraco Huangyou #1 in the early stage, so that the flesh content of the hybrid pelteobagrus fulvidraco Huangyou #1 is effectively increased, and the breeding economic benefits of the hybrid pelteobagrus fulvidraco Huangyou #1 are remarkably improved. The method is accurate, reliable and easy and convenient to operate.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the growth traits of the hybrid yellow catfish "Huangyou No. 1" and its application. The hybrid yellow catfish "Huangyou No. 1" can be selected by using the SNP molecular marker with a body type with a high meat content ", which belongs to the field of genetics and breeding of yellow catfish. Background technique [0002] Molecular marker-assisted breeding uses molecular markers closely linked to target traits for indirect selection. It selects target traits at the molecular level. It has high selection intensity, is not affected by the environment, and has reliable results, which can accelerate the breeding process. As a new generation of molecular markers, single nucleotide polymorphism (SNP) has been recognized in the field of breeding. It has the advantages of high polymorphism, genetic stability and convenient detection, and has been widely used in the field of molecular breeding of animal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6858
Inventor 尹绍武武兆文张鑫宇王宏玉裴雪莹王涛
Owner NANJING NORMAL UNIVERSITY
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