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Method for cultivating salt-tolerant flora in circulating aquaculture system of Maccullochella peelii

A recirculating aquaculture and dragon-patterned technology, applied in biochemical equipment and methods, fish farming, microorganisms, etc., can solve problems such as low salinity tolerance of microbial flora, reduce the concentration of ammonia nitrogen and other harmful substances, avoid Death, guaranteed stability effects

Pending Publication Date: 2019-06-11
南通龙洋水产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low salinity tolerance of microbial flora in the Longwenban breeding system, and provide a kind of salt-tolerant bacteria that can live in both fresh water and low-salinity seawater through manual technical intervention. group breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this embodiment, the place of implementation is a certain Longwenban breeding base, and the water temperature is 15°C. The implementation steps are as follows.

[0024] (1) Raise Longwenban in the recirculating aquaculture system at a salinity of 0‰, the selected fish body size is 500g / tail, and the stocking density is 20kg / m 3 , raised for 10 days, and formed a fixed flora. Through on-site testing, the stable flora density was 25,200 cfu / L.

[0025] (2) Adjust the salinity of the water body to 2‰, so that the flora gradually adapts to the environment with salinity, and raise them for 20 days to form a stable flora resistant to salinity.

[0026] (3) Adjust the salinity of the water body to 5‰, adapt the flora to this salinity, and raise it for 20 days to form a stable flora.

[0027] (4) Adjust the salinity of the water body to 7‰, adapt the flora to this salinity, and raise it for 20 days to form a stable flora.

[0028] (5) Adjust the salinity of the water body...

Embodiment 2

[0034] In this embodiment, the place of implementation is a certain Longwenban breeding base, and the water temperature is 20°C. The implementation steps are as follows.

[0035] (1) Raise Longwenban in the recirculating aquaculture system at a salinity of 0‰, the size of the selected fish is 500g / tail, and the stocking density is 25kg / m 3 , raised for 15 days, and formed a fixed flora. Through on-site testing, the stable flora density was 38,200 cfu / L.

[0036] (2) Adjust the salinity of the water body to 2.5‰, so that the flora gradually adapt to the environment with salinity, and raise them for 25 days to form a stable flora resistant to salinity.

[0037] (3) Adjust the salinity of the water body to 5.5‰, adapt the flora to this salinity, and raise it for 25 days to form a stable flora.

[0038] (4) Adjust the salinity of the water body to 7.5‰, adapt the flora to this salinity, and raise it for 25 days to form a stable flora.

[0039] (5) Adjust the salinity of the wat...

Embodiment 3

[0045] In this embodiment, the place of implementation is a certain Longwenban breeding base, and the water temperature is 25°C. The implementation steps are as follows.

[0046] (1) Raise Longwenban in the recirculating aquaculture system at a salinity of 0‰, the size of the selected fish is 500g / tail, and the stocking density is 30kg / m 3 , reared for 20 days, and formed a fixed flora. Through on-site testing, the stable flora density was 41,800 cfu / L.

[0047] (2) Adjust the salinity of the water body to 3‰, so that the flora gradually adapt to the environment with salinity, and raise them for 30 days to form a stable flora resistant to salinity.

[0048] (3) Adjust the salinity of the water body to 6‰, adapt the flora to this salinity, and raise it for 30 days to form a stable flora.

[0049] (4) Adjust the salinity of the water body to 8‰, adapt the flora to this salinity, and raise it for 30 days to form a stable flora.

[0050] (5) Adjust the salinity of the water bod...

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Abstract

The invention discloses a method for cultivating salt-tolerant flora in a circulating aquaculture system of Maccullochella peelii. The method mainly comprises the following steps: firstly culturing fixed flora in fresh water culture, then gradually screening out adaptive flora through stepped salinity improvement, culturing out biological flora with a strong tolerance range, and finally culturingout salt-tolerant flora in a repeated screening manner. By means of the method, the dragon spots can be prevented from being invaded by the ichthyophthiriasis, and an original ecological chain is protected.

Description

technical field [0001] The invention relates to the fields of fast aquaculture and microbial cultivation, in particular to a method for cultivating salt-tolerant bacteria in a dragon-striped aquaculture system. Background technique [0002] Dragon stripe, native to Australia, has a large body and weak swimming ability. It is one of the largest freshwater fish in the world. Through domestic introduction and artificial cultivation, researchers have found that this fish species is especially suitable for intensive farming. Dragon's spot has strong disease resistance, and its origin is mainly invasive fish disease, but the most harmful one in domestic intensive aquaculture is infectious small melon worm disease, when the water temperature is 12-27°C when it occurs, in intensive aquaculture During the process, because the seedlings are placed too densely, once the supply of bait exceeds the demand, and the dissolved oxygen is lower than 3.5mg / L for five consecutive days, it will ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00A01K61/10
CPCY02A40/81
Inventor 钱晓明秦桂祥陈义培朱新鹏史磊磊张文龙张巧云
Owner 南通龙洋水产有限公司
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