Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tuberculosis detection kit and application thereof

A technology for detecting kits and tuberculosis, which can be used in measuring devices, biological packaging, instruments, etc., and can solve problems such as inaccuracy and long detection process

Active Publication Date: 2019-06-21
PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the current traditional tuberculosis detection that requires imaging detection combined with other tests, the detection process is long and inaccurate. Most of the existing colloidal gold method detection kits are disposable, and there is no reusable colloidal gold test paper to detect tuberculosis reagents. The technical problem of the box, the present invention aims to provide a tuberculosis detection kit and its application. Using the detection kit, with the step-shaped partition, the preparatory work for colloidal gold detection can be quickly completed, and it has repeatability. The double-antibody sandwich method detects whether there is tuberculosis antigen in the serum sample. With the tilt setting of the workbench, it has high specificity, high sensitivity and high accuracy detection performance, and can quickly screen out positive samples with tuberculosis antigen serum, which is fast and convenient. Ground-assisted diagnosis of pulmonary tuberculosis, the detection time is 3-5 minutes, and has a wide range of practicality and development value in the field of disease detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tuberculosis detection kit and application thereof
  • Tuberculosis detection kit and application thereof
  • Tuberculosis detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment one: pulmonary tuberculosis detection kit

[0034] Such as Figure 1-4 As shown, a tuberculosis detection kit includes a box body 1 and a cover body 3, the cover body 3 is arranged above the box body 1, and the inside of the box body 1 is divided into a material area and a reagent area 4 by a partition 213, and in the material area A stepped partition 2 is provided, and the sample film area 24, the colloidal gold pad area 23, the acid cellulose film area 22 and the tool area 21 are sequentially arranged on the stepped partition 2 from top to bottom. The box body 1 is provided with a working table 12, and the working table 12 is arranged obliquely. The reagent area 4 is provided with a partition 3 45, and a sealing liquid bottle 41, an anti-gold standard antibody bottle 42, and a washing liquid bottle 43 are arranged on the partition 3 45. And antibody bottle 44, temperature control box 451 is set movable in partition three 45.

[0035] In the present invent...

Embodiment 2

[0041] Embodiment two: the application of pulmonary tuberculosis detection kit

[0042] An application method of a pulmonary tuberculosis detection kit, specifically comprising the following steps:

[0043] (1) Collect 5ml of venous blood from the examiner, centrifuge at 1500r / min-3000r / min for 5min-15min, and then take the upper serum as a serum sample;

[0044] (2) Pull out the workbench 12 from the side of the detection kit through the handle 123, open the cover 3 through the handle 33, take out the sanitary tweezers, the working board 125 and the rubber strip 126 from the tool area 21, and use the sanitary tweezers to clamp the working board 125, align the fixing hole 128 with the fixing column 122, put it into the working board 125, use sanitary tweezers to place the rubber strip 126 on the upper surface of the working board 125, and flatten it;

[0045] (3) Sampling is carried out in the order that the step-shaped partition 2 is upward. First, use hygienic tweezers to t...

Embodiment 3

[0049] Embodiment 3: Effect experiment of tuberculosis detection kit

[0050] The subjects of this study were selected from 150 PTB patients who were treated from June 2017 to June 2018. All patients were accompanied by symptoms of cough and sputum, and the duration of the disease was more than 14 days. Based on the "Guidelines for Clinical Diagnosis and Treatment - Tuberculosis" compiled by the Chinese Medical Association Patients diagnosed with pulmonary tuberculosis in the Fascicle; 30 patients with non-tuberculosis other respiratory diseases who were treated at the same time; patients with immune system diseases and patients who used immunosuppressant drugs in the near future were excluded from the two groups of patients; and all signed the informed consent willing to participate in this research.

[0051] 3.1 Diagnostic sensitivity

[0052] 100 serums of patients diagnosed as PTB were collected from the clinic, and the serum of the PTB patients collected above was tested...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tuberculosis detection kit and application thereof. The kit comprises a kit body and a cover body, the cover body is arranged above the kit body, the interior of the kit bodyis divided by a second partition board into a material area and a reagent area, a step partition board is arranged in the material area, the step partition board is sequentially provided with a sample film area, a colloidal gold pad area, an acid cellulose membrane area and a tool area from top to bottom, a worktable is arranged at the position, below the step partition board, on the kit body andis inclined, the detection kit is used, in cooperation with the step partition board, preparing work of colloidal gold method detection can be rapidly finished, the repeated operation is achieved, adouble antibody sandwich method is used for detecting whether tuberculosis antigens exist in serum samples or not, in cooperation with inclination of the worktable, highly-peculiar, highly-sensitive and highly-accurate detection performance is achieved, positive samples with tuberculosis antigen serum can be rapidly screened out, auxiliary diagnosis of tuberculosis can be rapidly, and convenientlycarried out, the detection time ranges from three to five minutes, and the kit has the wide practicability in the disease detection field.

Description

technical field [0001] The present invention mainly relates to the technical field of immunodiagnosis, specifically, the present invention relates to the technical field of a tuberculosis immunodiagnostic kit. Background technique [0002] The World Health Organization released the "Global Tuberculosis Report 2018" on September 18, 2018, showing that in 2017, there were approximately 1.7 billion latent tuberculosis infected people in the world, and the latent infection rate was 23%. There are about 10 million new tuberculosis patients in the world, and the incidence rate of tuberculosis is 133 / 100,000. Among them, children younger than 15 years old and HIV-infected patients account for 10% and 9% of new tuberculosis patients respectively; Newly diagnosed patients accounted for 87.2% of the world's total; new patients in India (27.4%), China (8.9%), Indonesia (8.4%) and the Philippines (5.8%) accounted for about 50% of the world's new patients. The estimated number of new tu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B65D85/62B65D25/06B65D81/36G01N33/558
CPCY02W90/10
Inventor 李奇凤张力鹏
Owner PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com