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A kind of pulmonary tuberculosis detection kit and application method thereof

A technology for detecting kits and tuberculosis, which is applied in the direction of measuring devices, instruments, biological packaging, etc., can solve problems such as inaccuracy and long detection process, and achieve the effects of convenient auxiliary diagnosis, extensive practicability and development value

Active Publication Date: 2021-05-11
PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the current traditional tuberculosis detection that requires imaging detection combined with other tests, the detection process is long and inaccurate. Most of the existing colloidal gold method detection kits are disposable, and there is no reusable colloidal gold test paper to detect tuberculosis reagents. The technical problem of the box, the present invention aims to provide a tuberculosis detection kit and its application. Using the detection kit, with the step-shaped partition, the preparatory work for colloidal gold detection can be quickly completed, and it has repeatability. The double-antibody sandwich method detects whether there is tuberculosis antigen in the serum sample. With the tilt setting of the workbench, it has high specificity, high sensitivity and high accuracy detection performance, and can quickly screen out positive samples with tuberculosis antigen serum, which is fast and convenient. Ground-assisted diagnosis of pulmonary tuberculosis, the detection time is 3-5 minutes, and has a wide range of practicality and development value in the field of disease detection

Method used

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  • A kind of pulmonary tuberculosis detection kit and application method thereof
  • A kind of pulmonary tuberculosis detection kit and application method thereof
  • A kind of pulmonary tuberculosis detection kit and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Example 1: Tuberculosis test tester

[0034]Such asFigure 1-4As shown, a pulmonary tuberculosis detection test kit, including the casing 1 and the cover body 3, and the cover body 3 is disposed above the casing 1, and the spacer 1 is divided into material zone and reagent zone 4, within the material area. The step-shaped partition 2 is provided, and the sample film region 24, the colloidal gold pad region 23, the acid cellulose membrane region 22 and the tool area 21, and the step-shaped partition 2 are sequentially provided from top to bottom. A table 12 is provided on the casing 1, the table 12 is tilted, and the reagent zone 4 is provided with a separator three 45. The separator three 45 is provided with a closed liquid bottle 41, an anti-ferrous antibody bottle 42, a washing liquid bottle 43. The temperature control box 451 is disposed within the anti-antibody bottle 44 and the separator 3 45.

[0035]In the present invention, a groove 121 is provided on the table 12, and the pl...

Embodiment 2

[0041]Example 2: Application of tuberculosis test tester

[0042]Application method of pulmonary tuberculosis test tester, specifically includes the following steps:

[0043](1) Collecting the detector's venous blood 5ml, 1500r / min-3000r / min centrifugation for 5 min-15 min after taking the upper serum as a serum sample;

[0044](2) Pull the work table 12 from the inspection kit side by handle 123, by opening the cover 3 by the handle 33, remove the hygiene tweezers, work plate 125 and the strip 126 from the tool area 21, and use the sanitary tweezers to clamp the workpiece 125. The fixing hole 128 is aligned with the fixing column 122, placed in the workpiece 125, and the strip 126 is placed on the surface of the workpiece 125, leveling;

[0045](3) Sampling According to the order of steps 2, first, the absorbent film 212 is taken out from the tool region 21 by the sanitary tweezers 211, followed by an acid fiber membrane region 22 to take out an acid fiber. Plasma film 221, the bottom end ...

Embodiment 3

[0049]Example 3: Experiment of the effect of pulmonary tuberculosis test tester

[0050]This research object is selected from 150 patients with PTB in June 2017, June 2018, all patients have cough, cough symptoms, and more than 14 days, combined with the Chinese Medical Association - Tuberculosis Visual diagnosis is a tuberculosis patient; 30 patients with other respiratory diseases of non-tuberculosis in the same period; two groups of patients were excluded with patients with immune system, and patients with immunosuppressive drugs were used in the near future; and they signed an informed consent Willing to participate in this study.

[0051]3.1 Diagnostic sensitivity

[0052]From the clinical collected from serum diagnosed as PTB patients, the serum of PTB patients collected above was detected by the operation step of the application method of Example 2 with the operation step of the application method of Example 2. If there is a naked spot, there is a nick spot, it is judged as positive. ...

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Abstract

The invention discloses a pulmonary tuberculosis detection kit and its application. The kit includes a box body and a cover body. A cover body is arranged above the box body. Separator, the sample film area, colloidal gold pad area, acid cellulose film area and tool area are arranged sequentially from top to bottom on the stepped partition, and a workbench is provided on the box below the step-shaped partition, and the workbench is inclined Setting; using this detection kit, with the step-shaped partition, can quickly complete the preparation of the colloidal gold method detection, with repeatability, use the double-antibody sandwich method to detect whether there is tuberculosis antigen in the serum sample, and cooperate with the inclined setting of the workbench , with high specificity, high sensitivity, and high accuracy detection performance, can quickly screen out positive samples with tuberculosis antigen serum, can quickly and conveniently assist in the diagnosis of pulmonary tuberculosis, and the detection time is 3‑5min, and has a wide range of applications in the field of disease detection practicality.

Description

Technical field[0001]The present invention mainly relates to the technical field of immunization diagnosis, and in particular, the present invention relates to the technical field of immunization diagnostic kits of tuberculosis.Background technique[0002]World Health Organization released "2018 Global Tuberculosis Report" on September 18, 2018, showing that the global tuberculosis latent infected population is approximately 1.7 billion, the latent infection rate is 23%. There are about 10 million patients with new nuclear diseases in the world, and the incidence of tuberculosis is 133 / 100. There are 10% and 9% of children with children aged 15 years of age account for 10% and 9% of new patients; 30 tuberculosis high burden of national new The number of patients accounted for 87.2% of the world; India (27.4%), China (8.9%), Indonesia (8.8%) and the new new patients in the Foundation of the Foundation account for approximately 50% of the world. China's estimated tuberculosis has a numb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B65D85/62B65D25/06B65D81/36G01N33/558
CPCY02W90/10
Inventor 李奇凤张力鹏
Owner PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION
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