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Contact biomaterial DNA collection and storage device and method thereof

A technology of biological testing materials and storage devices, which is applied in the field of DNA collection, can solve the problems of affecting the detection results, easily floating on the supernatant, and affecting the efficiency of detection, so as to reduce the chance of deterioration and reduce the drop of the shell The probability of breaking, the effect of convenient rotation collection

Pending Publication Date: 2019-07-12
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the detection process, the head of the cotton swab needs to be cut off and placed in a centrifuge for centrifugation, but the existing cotton swab still contains a bamboo stick even if the head is cut off, and it is placed in a centrifuge, but the bamboo stick has buoyancy , easy to float on the supernatant, resulting in relevant DNA sticking to the bamboo sticks, thus affecting the detection results. The existing treatment method is to detect more than a dozen cotton swab heads for comprehensive judgment, which affects the detection efficiency

Method used

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  • Contact biomaterial DNA collection and storage device and method thereof
  • Contact biomaterial DNA collection and storage device and method thereof
  • Contact biomaterial DNA collection and storage device and method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] see Figure 1-8 According to an embodiment of the present invention, a device for collecting and storing DNA of contact biological samples includes a housing 1, and one end of the housing 1 is provided with a first threaded groove 2, and a cooling device is provided in the first threaded groove 2. Mechanism 3, the cooling mechanism 3 includes an ice storage cylinder 4, the ice storage cylinder 4 is provided with threads, the ice storage cylinder 4 matches the first thread groove 2 through the threads, and the ice storage cylinder 4 One end of the cylinder 4 is fixed with a rotating baffle 5, the rotating baffle 5 is matched with the housing 1, a sealing door 6 is opened on the ice storage cylinder 4, and one side of the first threaded groove 2 An annular cavity 7 is opened on the housing 1, and the annular cavity 7 communicates with the first thread groove 2. A storage cavity 8 is opened in the housing 1. It is worth noting that, The storage cavity 8 can be cooled in a...

Embodiment 2

[0047] see figure 1 with Figure 4 , for the rotating baffle 5 , the end of the rotating baffle 5 away from the ice storage cylinder 4 is fixedly provided with a lever handle 28 . The housing 1 is provided with a foam insulation cover 29 . The outer cover of the handle glove 15 is provided with a replaceable disposable sterile cotton cover 30 . A swivel 31 is fixed on one side of the handle glove 15 on the central axis 13 . A number of ventilation holes 32 are opened around the transparent cover 10 . By arranging the leverage handle 28, the convenient rotation of the rotating baffle 5 is increased, and by arranging the foam insulation cover 29, the temperature in the storage cavity 8 is further increased to maintain a certain level, and the casing 1 can also be reduced from falling. The probability of failure, by setting the disposable sterile cotton cover 30, the cleanliness of the handle glove 15 is increased, by setting the leverage swivel 31, the rotation of the centra...

Embodiment 3

[0049] see Image 6 , Figure 7 with Figure 8 , for the limit mechanism 24, the limit mechanism 24 includes a threaded barrel 33, the threaded barrel 33 is sleeved on the extension barrel 20, and a nut 34 is threaded on the threaded barrel 33, and the threaded barrel 33 is threaded. One end of the barrel 33 is fixed with two arc-shaped threaded plates 35, the arc-shaped threaded plates 35 match the nuts 34, and an arc-shaped limiting plate 36 is arranged under the arc-shaped threaded plates 35, The inner wall of the arc-shaped limiting plate 36 is provided with several locking teeth. The collection cotton sheet 23 includes a cotton layer 37 and two cover mesh layers 38 , and the cover mesh layers 38 are attached to both sides of the cotton layer 37 respectively. The cover 27 is fixed with a U-shaped handle 39 . By setting the limit mechanism 24, the connection stability between the collection cotton piece 23 and the disposable replaceable core body 19 is increased, and th...

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Abstract

The invention discloses a contact biomaterial DNA collection and storage device and a method thereof. The device comprises a housing, one end of the housing is provided with a first thread groove, a cooling mechanism is arranged in the first thread groove, the cooling mechanism comprises an ice storage barrel, the outer side of the ice storage barrel is provided with a thread, the ice storage barrel is matched with the first thread groove through the first thread, a rotary baffle is fixedly arranged at one end of the ice storage barrel, the rotary baffle is matched with the housing, the ice storage barrel is provided with a sealing door, the portion, on one side of the first thread groove, of the housing is provided with an annular cavity, the annular cavity is communicated with the firstthread groove, a storage cavity is formed in the housing, and the storage cavity is located in the annular cavity. The contact biomaterial DNA collection and storage device and the method thereof havethe advantages that the influence of bamboo sticks on DNA is avoided, the accuracy of detection results is improved, the number of times of detecting samples is reduced, the results closest to true values are obtained, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the field of DNA collection, in particular to a device for collecting and preserving DNA of contact biological specimens and a method for using the same. Background technique [0002] With the development of DNA gene sequencing technology, forensic science DNA technology has been widely used in crime scene investigation. In recent years, the pace of DNA database construction of public security organs across the country has been accelerating, and it is playing an increasingly important role in combating crime and protecting people's lives and property. Since the development of DNA analysis in our country, the application of this technology has successfully identified a large number of difficult cases, and played an important role in the detection of many criminal cases and the resolution of civil cases. [0003] At the crime scene, for most contact biological samples that cannot be directly extracted in kind, cotton swabs are u...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/12C12M1/02C12M1/00C12Q1/6806
CPCC12Q1/6806
Inventor 范浩亮邱平明赵凯王骁段利忠程诚
Owner HAINAN MEDICAL COLLEGE
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