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Urine sulfhydryl compound mercury-free detection test strip and preparation method

A mercapto compound, detection test paper technology, applied in the direction of analysis by chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc. The detection rate is accelerated, the use safety is improved, and the operability is strong.

Inactive Publication Date: 2019-07-12
SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing mercapto group detection technology in urine mainly uses the phosphotungstic acid method to achieve detection, such as Chinese patent: CN107576652A A detection reagent for mercapto compounds in urine and its preparation method and use, CN105115966A Detection reagent for mercapto compounds in urine box and its preparation method and application and CN106468715A a cervical cancer urine detection reagent and its preparation method and application, the method has the following defects: 1) the detection method contains mercury ions, for this manually operated sample addition product, The presence of mercury is extremely toxic. In addition, the detection of heavy metal ions such as mercury and nickel in the waste liquid will also affect the environment and require special treatment; 3) The mercury ions in the reagent are easily affected by the high concentration of uric acid in the urine, which will cause great interference and cause false negative results. 4) At present, the detection kits are all packaged in ampoule liquid, and then mixed with urine Inspection, this kind of packaging is bulky, inconvenient to transport, and has a lot of waste

Method used

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  • Urine sulfhydryl compound mercury-free detection test strip and preparation method
  • Urine sulfhydryl compound mercury-free detection test strip and preparation method

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Experimental program
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Effect test

Embodiment 1

[0024] 1) Prepare buffer solution: Weigh 1.5g sodium dihydrogen phosphate and 70.22g disodium hydrogen phosphate dodecahydrate, add water to make the volume to 1L, and the pH value of the buffer solution is 7.8.

[0025] 2) Weigh 6.14g of 5,5'-dithiobis(2-nitrobenzoic acid) (DNTB) (6.14g / L), 0.32g of disodium ethylenediaminetetraacetic acid (EDTA) (320mg / L L), 10g of 2-methyl-2,4-pentanediol (15g / L), 10g of silicotungstic acid (10g / L), 14g of polyethylene glycol and 1.7g of Triton X-100, the above group Partially added to the prepared buffer;

[0026] 3) After the components prepared in step 2) are completely dissolved, soak the filter paper in the solution, take it out and dry it, cut it into small pieces, and stick it on the plastic strip with double-sided tape.

Embodiment 2

[0028] 1) Prepare buffer solution: Weigh 1.5g sodium dihydrogen phosphate and 70.22g disodium hydrogen phosphate dodecahydrate, add water to make the volume to 1L, and the pH value of the buffer solution is 7.8.

[0029] 2) Weigh 6.14g of 5,5'-dithiobis(2-nitrobenzoic acid) (DNTB) (6.14g / L), 0.32g of disodium ethylenediaminetetraacetic acid (EDTA) (320mg / L L), 15g of 2-methyl-2,4-pentanediol (15g / L), 6g of silicotungstic acid (10g / L), 14g of polyethylene glycol, 1.7g of Triton X-100, 2mL Green (0.1%) dye, add the above components into the prepared buffer;

[0030] 3) After the components prepared in step 2) are completely dissolved, soak the filter paper in the solution, take it out and dry it, cut it into small pieces, and stick it on the plastic strip with double-sided tape.

Embodiment 3

[0031] Preparation and use of embodiment 3 standard color card

[0032] 1) Configure 1mmol / L, 0.5mmol / L, 0.1mmol / L, 0.04mmol / L, 0mmol / L cysteine ​​(Cys) standard solution;

[0033] 2) The cysteine ​​standard solution of each concentration configured in step 1) is immersed in 5 test strips prepared for use, and then taken out;

[0034] 3) Prepare a color comparison card according to the color development after 2 minutes;

[0035] 4) When using, dip the test strip into the sample to be tested and then take it out, add the sample to the reagent bottle according to the sample to be tested, and compare it with the standard color comparison card after 5 minutes of stable color change. The corresponding concentration is the concentration of the solution to be tested. If it is between the two color scales, the concentration of sulfhydryl groups is estimated.

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Abstract

The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a urine sulfhydryl compound mercury-free detection test strip and a preparation method. The test strip comprises filter paper, and DTNB and EDTA which are loaded on the filter paper. The preparation method comprises the following steps: preparing a buffer solution, dissolving components needing to be loaded on the filter paper in the buffer solution, applying the mixed solution to the filter paper by a soaking method, cutting the filter paper into small blocks after the filter paper is dried, and pasting the small blocks on a plastic strip for later use. According to the technical scheme provided by the invention, DTNB is used as a sulfydryl color developing agent and is successfully improved into a test strip type urine detection reagent; the test strip does not contain heavy metal ions such as mercury and corrosive substances such as trichloroacetic acid, so that the use safety is improved, the detection rate is greatly increased and is shortened from at least 15 minutes to within 5 minutes, and the problem of the requirement of test strip detection on the color development time is solved.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and in particular relates to a mercury-free detection test strip for urine mercapto compounds and a preparation method thereof. Background technique [0002] Among the malignant tumors that endanger women's health in the world, cervical cancer is listed third. According to statistics, in 2008, among all new cancers in women worldwide, the proportion of new cervical cancer was 9% (529,800 cases). ), second only to breast cancer and colorectal cancer. Among the patients who died of cancer among women, 8% (275,100 cases) of female cancer patients died of cervical cancer, ranking fourth. Among the patients who died of cervical cancer, about 230,000 people belonged to developing countries, accounting for about 83% of the total number. In my country, according to statistics, there are about 130,000 new cases of cervical cancer every year. [0003] During the whole process of cervical prec...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 刘东
Owner SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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