EMT dynamic monitoring system, EMT dynamic monitoring system containing EMT dynamic monitoring system and application thereof

A dynamic monitoring and system technology, applied in the field of biomedicine, can solve the problem that EMT cannot perform dynamic monitoring, and achieve the effect of breaking through the bottleneck of research.

Inactive Publication Date: 2019-07-19
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The present invention aims to provide an EMT dynamic monitoring system, including its EMT dynamic monitoring system and application, to solve the technical problem that EMT cannot perform dynamic monitoring in the prior art

Method used

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  • EMT dynamic monitoring system, EMT dynamic monitoring system containing EMT dynamic monitoring system and application thereof
  • EMT dynamic monitoring system, EMT dynamic monitoring system containing EMT dynamic monitoring system and application thereof
  • EMT dynamic monitoring system, EMT dynamic monitoring system containing EMT dynamic monitoring system and application thereof

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Embodiment 1

[0075] In this example, the part of the specific embodiment that is not described in detail is described in the specific embodiment part, such as the carrier and composition of the "EMT induction system", etc. The specific implementation method can use conventional technical means in the field.

[0076] The scheme in this embodiment is as follows:

[0077] 1. "EMT induction system" overexpressing TGF-β1

[0078] The cDNA of EMT-inducing molecule TGF-β1 was inserted into the reaction vector of Tet-On system.

[0079] 2. Construct an "EMT reporting system" based on Cre / loxP and bioluminescent technology

[0080] 1) Designing the promoter of the Cre gene as the promoter of the mesenchymal marker Vimentin;

[0081] 2) Construct two opposite LoxP sites (LoxP and antiLoxP), and insert reporter molecule A (forward) and reporter molecule B (reverse) between the pair of reverse LoxP sites. When there is no Cre recombinase action, the vector expresses the reporter molecule A; under t...

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Abstract

The invention discloses an EMT dynamic monitoring system, EMT dynamic monitoring system body containing the EMT dynamic monitoring system and an application thereof. The EMT dynamic monitoring systemis constructed based on a Cre/LoxP recombinase system, which includes: a Cre gene placed under the control of a promoter of an EMT-related gene; a conversion reporter vector comprising two opposite LoxP sites, wherein a first reporter molecule and a second reporter molecule are interposed between two opposite LoxP sites; alternatively, the conversion reporter vector comprises a pair of co-directional LoxP sites, the first reporter molecule and a termination signal are inserted between a pair of co-directional LoxP sites, and the second reporter molecule is inserted after the LoxP site. By applying the technical scheme of the invention, the EMT dynamic monitoring system based on Cre/loxP recombinase and a bioluminescence technology is established, real-time quantitative monitoring of tumorEMT animal model is realized, and the bottleneck of tumor EMT research is breakthrough.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an EMT dynamic monitoring system, an EMT dynamic monitoring system including the same and an application thereof. Background technique [0002] In the process of malignant tumor progression, epithelial cells will undergo mesenchymal-like morphological and functional changes, which is called EMT (Epithelial-Mesenchymal Transition, mesenchymal transition of epithelial cells). The specific manifestation is that epithelial cells lose the tight junction and polarity between cells, and gain the ability of invasion and migration. In recent years, EMT-related research has attracted much attention, because EMT is closely related to tumor drug resistance, recurrence, metastasis and tumor stem cell theory. Therefore, therapeutic strategies aimed at preventing EMT have become a research hotspot in the fields of biology and pharmacy in recent years. However, the current exploration of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6897C12N15/85
CPCC12Q1/6897C12N15/85C12N2800/107C12N2800/30
Inventor 李琳娜
Owner ACADEMY OF MILITARY MEDICAL SCI
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