Method for detecting palmatine and berberine in Chinese patent medicine

A technology of berberine and Chinese patent medicine, applied in the field of drug analysis, can solve the problems of poor selectivity and low sensitivity, and achieve the effect of high selectivity, high sensitivity, fast and efficient determination

Active Publication Date: 2019-08-02
SHANXI UNIV OF CHINESE MEDICINE
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  • Description
  • Claims
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Problems solved by technology

[0004] In order to solve the problems of low sensitivity and poor selectivity in the detection of berberine and palmatine, the present invention provides a method for measuring palmatine and berberine in Chinese patent medicines

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  • Method for detecting palmatine and berberine in Chinese patent medicine
  • Method for detecting palmatine and berberine in Chinese patent medicine
  • Method for detecting palmatine and berberine in Chinese patent medicine

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with specific examples.

[0017] A method for measuring palmatine and berberine in Chinese patent medicines, comprising the steps of:

[0018] (1) Test preparation: 60 mmol L of buffer solution with pH=7 ‒1 Na 2 HPO 4 and 35% methanol in water; laser-induced fluorescence detector: excitation wavelength λ ex =488 nm, emission wavelength λ em =520 nm; Prepare chromatographic conditions: the capillary column used in the experiment is 75 μm × 60.00 cm; the detection and separation voltage is 24 kv, the injection pressure is 0.5 psi, the injection volume is 3~5s, and the temperature is 25°C;

[0019] (2) Preparation of stock solution: Weigh a certain amount of berberine, palmatine standard and cucurbituril [7], and prepare a certain concentration of 445 μg / mL berberine with the buffer solution in step (1) standard stock solution and 450 μg / mL palmatine standard stock solution; more than 3 times; becau...

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Abstract

The invention discloses a method for detecting palmatine and berberine in Chinese patent medicine and relates to the field of pharmaceutical analysis. The adopted principle is that active ingredient berberine and bamatine isoquinoline alkaloids have no fluorescence, and cucurbituril [7] (CB [7]) itself has no fluorescence; and however, the CB [7] can form a stable inclusion compound with the berberine and bamatine, and can allow the fluorescence sensitivity of the berberine and bamatine without fluorescence to be greatly increased and the fluorescence of a solution to be obviously enhanced. With the help of good separation ability of capillary electrophoresis, the inclusion compound formed by the berberine and bamatine and the CB [7] can also realize good separation without being influenced by other substances; and the method has good selectivity and is higher in sensitivity than an ultraviolet detection method by two orders of magnitude. The capillary electrophoresis / fluorescence analysis detection method has high sensitivity, good selectivity and strong anti-interference ability; compared with the existing capillary electrophoresis / ultraviolet analysis detection method, the method is higher by two orders of magnitude; and the method can also be used for analysis determination of the palmatine and berberine in biological samples.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a method for determining palmatine and berberine in Chinese patent medicines. Background technique [0002] Berberine and palmatine are isoquinoline alkaloids with very similar structures, which mainly exist in the rhizomes of natural Chinese herbal plants such as Coptidis Rhizoma, Cortex Phellodendron, Sankezhen and Nandina. Berberine and palmatine have many pharmacological activities, and can be used as antibacterial drugs, plasmodium drugs, antidiarrheal drugs, and cardiovascular drugs. There are also many separation and determination methods for berberine and palmatine, such as: capillary electrophoresis ultraviolet detection (CE-UV), near infrared spectroscopy (NIRS), ion-pair supercritical fluid chromatography (IP-SFC), flow injection Micellar Electrochromatography (MEKC), High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS). The s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/64G01N30/78G01N21/64
CPCG01N30/02G01N30/74G01N30/64G01N30/78G01N21/643
Inventor 常银霞赵娅丽魏砚明刘鑫
Owner SHANXI UNIV OF CHINESE MEDICINE
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