SNP molecular markers located on pig chromosome 16 related to pig lean meat percentage and eye muscle area and their application

An eye muscle area and molecular marker technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of little effect and long time, and achieve the effect of improving the breeding process, increasing the price, and speeding up the breeding process.

Active Publication Date: 2020-10-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, improving these two traits through traditional selection methods is time-consuming and has little effect

Method used

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  • SNP molecular markers located on pig chromosome 16 related to pig lean meat percentage and eye muscle area and their application
  • SNP molecular markers located on pig chromosome 16 related to pig lean meat percentage and eye muscle area and their application
  • SNP molecular markers located on pig chromosome 16 related to pig lean meat percentage and eye muscle area and their application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 explains in detail the determination process that affects lean meat percentage in the present invention

[0037] The lean meat percentage of live pigs with a body weight of 100kg was determined by the New Zealand HGS (Hennessy Grading System) carcass grading system. Aloka SSD 500V in vivo B-ultrasound measuring instrument was used to measure the eye muscle area of ​​all test pigs when they were 100kg, and the measurement site was the 10th to 11th intercostal space. The measurement results are corrected by software for the area of ​​the 100kg eye muscle. Use the standard measurement software package to calculate the eye muscle area, and directly print out the image and measurement value.

[0038] The experimental pig group used in the present invention is 2127 purebred plus-line Duroc of Guangdong Wen's Food Group Co., Ltd. Breeding Pig Branch Company, which is the core group of the Breeding Pig Branch Company, and the group pedigree records are detailed. ...

Embodiment 2

[0039] Embodiment 2 specifically explains the inventive process of obtaining the gene marker in the present invention

[0040] (1) The method of extracting DNA from the ear-like tissues of the Canadian Duroc pig was extracted from the whole genome DNA according to the marked phenol-chloroform method. Using Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred plus line Duroc population. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0041] (2) 50K SNP genotype detection of the whole pig genome: GeneSeek Genomic Profiler Porcine50KSNP typing platform, using Illumina Infinium instructions and standard procedures for chip hybridization and result scanning. Finally, the genotype data were read by GenomeStudio software. The quality control of the obtained genotype data was carried out with PLINK v1.07, and the detection rate of eliminat...

Embodiment 3

[0047] Embodiment 3 specifically explains the inventive process of inventing and detecting SNP markers

[0048] (1) The target segment containing the SNP site significantly related to the performance of the Canadian Duroc 100kg body weight eye muscle area and 100kg body weight lean meat rate is a 239bp nucleotide sequence in chromosome 16, and the upstream and downstream primers for sequence amplification It is primer-F and primer-R, and its nucleotide sequence is as follows:

[0049] Upstream primer primer-F: 5'-AGGTCAGAGTGGGGCAGTAA-3';

[0050] Downstream primer primer-R: 5'-AATGGGATTGGATGCGGGTT-3'.

[0051] (2) PCR amplification system and condition setting

[0052] Configure a 10 μL system, including 1 μL DNA sample, 0.3 μL upstream primer, 0.3 μL downstream primer, 5 μL PCR mix, ddH 2 O 3.4 μL, PCR conditions were pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 64°C for 30 s, extension at 72°C for 30 s, a total of 35 cycles, and the fina...

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Abstract

The invention belongs to the technical field of molecular biology and molecular markers, and particularly relates to an SNP molecular marker located at a chromosome 16 of a pig and related to the leanmeat percentage and eye muscle area of a pig and application. An SNP locus of the SNP molecular marker located at the chromosome 16 of the pig and related to the lean meat percentage and eye muscle area of the pig corresponds to A>C mutation of the 33,757,844bp of the chromosome 16 of the international pig reference genome of the 11.1 version; the molecular marker is obtained through correlation analysis of a whole genome and can significantly influence the characters of the eye muscle area and lean meat percentage of the pig when the weight of the pig is 100 kilograms. The invention further provides a primer pair used for identifying the molecular marker, by utilizing the molecular marker and the primer pair, an efficient and accurate molecular marker-assisted breeding technology canbe established, the SNP molecular marker is applied to genetic improvement of the characters of the eye muscle area and lean meat percentage of the pig, therefore the eye muscle area and lean meat percentage of the pig are increased, the profit of enterprises is increased, and the core competitiveness is improved.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker located on pig chromosome 16 and related to pig lean meat percentage and eye muscle area and its application. Background technique [0002] At present, the global breeding goal has changed from fat pigs to lean pigs, and improving the lean meat rate is the main breeding goal of pigs. The eye muscle area is one of the important indicators to measure the lean meat percentage, and these two traits are positively correlated, so breeders also increase the lean meat percentage by increasing the eye muscle area of ​​pigs. For decades, people have used traditional breeding methods to increase eye muscle area and increase lean meat percentage, which has improved these two traits to a certain extent. However, eye muscle area and lean meat percentage are complex quantitative traits regulated by multiple genes. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨杰吴珍芳周身娉蔡更元郑恩琴徐铮
Owner SOUTH CHINA AGRI UNIV
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