Targeting T lymphocyte and preparation method and application thereof

A lymphocyte, targeting technology, applied in the field of medical biology, can solve the problems of limited clinical application, tumor cell identification, and reduced killing ability, and achieve the effect of avoiding escape, expanding broad-spectrum killing, and enhancing identification.

Active Publication Date: 2019-08-23
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although CAR-T technology has achieved remarkable curative effects in hematological tumors such as leukemia, myeloma, and lymphoma, its ability to recognize and kill tumor cells in a complex tumor microenvironment (such as the simultaneous appearance of multiple tumor antigens) The ability is greatly reduced, limiting its clinical application

Method used

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  • Targeting T lymphocyte and preparation method and application thereof
  • Targeting T lymphocyte and preparation method and application thereof
  • Targeting T lymphocyte and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment 1 A method for preparing targeted T lymphocytes, comprising the following steps:

[0098] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD38 targeting CD38

[0099] Prepare signal peptide, single-chain antibody targeting CD38, CD8α hinge region, CD8 transmembrane region, 4-1BB signal region and CD3ζ signal region encoding genes respectively, in the CAR-CD38, the signal peptide encoding gene is as shown in SEQ ID As shown in NO: 22, the coding gene of the single-chain antibody targeting CD38 is shown in SEQ ID NO: 3, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 10, and the coding gene of the CD8 transmembrane region is shown in As shown in SEQ ID NO: 12, the coding gene of the 4-1BB signal region is shown in SEQ ID NO: 14, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 16.

[0100] The genes encoding the above signal peptide, CD38-targeting single-chain antibody, CD8α hinge region, CD8 transmembrane re...

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PUM

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Abstract

The invention provides a targeting T lymphocyte. The targeting T lymphocyte comprises a chimeric antigen receptor CAR-CD38 targeting CD38 and / or a chimeric antigen receptor CAR-BCMA targeting BCMA. The targeting T lymphocyte has wide and high targeting identifiability on tumor cells, target spot escape of the tumor cells can be avoided, the tumor cells are efficiently and specifically killed, andthe broad-spectrum destruction of the targeting T lymphocyte is enhanced. The invention further provides a preparation method and application of the targeting T lymphocyte.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a targeted T lymphocyte and its preparation method and application. Background technique [0002] Multiple myeloma (Multiple myeloma, MM) is a kind of abnormal proliferation of plasma cells, resulting in the invasion of a malignant tumor of the bone marrow. It is a malignant plasma cell disease that occurs in B lymphocytes, often accompanied by multiple osteolytic lesions, hypercalcemia, anemia, kidney damage and bacterial infection. In recent years, the incidence rate has increased and the age of onset has advanced. [0003] CAR-T (Chimeric Antigen Receptor T Cell) technology is a new type of immune cell therapy, which is to reinfuse CAR-modified T cells into the human body, activate the autoimmune system, and kill tumor cells to achieve the elimination of malignant tumors. purpose of tumor cells. Although CAR-T technology has achieved remarkable curative effects in hematologic...

Claims

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Application Information

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IPC IPC(8): C12N5/10C07K19/00C12N15/62C12N15/867A61P35/00C12Q1/02
CPCC12N5/0636C07K16/2896C07K16/2878C07K14/7051C07K14/70517C07K14/70578C12N15/86A61K35/17A61P35/00G01N33/5011C12N2510/00C07K2317/622C07K2319/00C07K2319/02C07K2319/03C07K2319/33C07K2319/74C12N2740/15043C12N2800/107C12N2503/02G01N2500/10
Inventor 张宏玲龙丽梅
Owner SHENZHEN BINDEBIOTECH CO LTD
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