Separation and purification method of crocin-1 and application of crocin-1

A technology for separation and purification of crocin, applied in chemical instruments and methods, organic chemistry, esterification and saccharide, etc., can solve problems such as no experiments can prove, and achieve reduced loss, reduced degradation, and reduced process operations. Effect

Pending Publication Date: 2019-09-27
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific mechanism has not yet been proved experimentally.

Method used

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  • Separation and purification method of crocin-1 and application of crocin-1
  • Separation and purification method of crocin-1 and application of crocin-1
  • Separation and purification method of crocin-1 and application of crocin-1

Examples

Experimental program
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Effect test

Embodiment example 1

[0031] Mix 10.0 g of saffron with 20% ethanol solution, mix well and stir evenly, and extract by ultrasonic for 40 minutes at 40°C to obtain the crude extract of saffron-1; remove the alcohol in the solvent by rotary evaporation, add water to constant volume volume, then add 10BV crocin-1 crude extract, and purify by connecting XAD 4 and XAD 1600N resins in series, wherein the volume ratio of XAD 4 and XAD1600N is 1:1 to obtain higher purity crocin -1 (crocin-1), the chromatographic purity of crocin-1 was 82.1%, and the yield was 10.09%.

Embodiment example 2

[0033] Mix 10.0 g of saffron with 40% ethanol solution, mix well and stir evenly, and extract by ultrasonic for 30 minutes at 50°C to obtain the crude extract of saffron-1; remove the alcohol in the solvent by rotary evaporation, add water to constant volume volume, then add 30BV crocin-1 crude extract, and purify by connecting XAD 4 and XAD 7HP resins in series, wherein the volume ratio of XAD 4 and XAD 7HP is 2:1 to obtain higher purity saffron Glycoside-1 (crocin-1), the chromatographic purity of crocin-1 was 88.2%, and the yield was 9.88%.

Embodiment example 3

[0035] Mix 10.0 g of saffron with 30% ethanol solution, mix well and stir evenly, and extract by ultrasonic for 30 min at 30°C to obtain the crude extract of saffron-1; remove the alcohol in the solvent by rotary evaporation, add water to constant volume volume, then add 20BV crocin-1 crude extract, and purify by connecting XAD 4 and ADS 7 resins in series, wherein the volume ratio of XAD 4 and ADS 7 is 4:1 to obtain higher purity saffron Glycoside-1 (crocin-1), the chromatographic purity of crocin-1 was 90.3%, and the yield was 8.52%.

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Abstract

The invention provides a separation and purification method of crocin-1. The separation and purification method comprises the steps as follows: mixing a plant material with an extraction solvent, performing ultrasonic extraction at 20-80 DEG C for 5-60 min, removing an alcohol solvent to obtain a crocin-1 crude extract, and then, purifying the crocin-1 crude extract by serial macroporous resin to obtain crocin-1, wherein the plant material is selected from crocus plants and/or gardenia plants; the extraction solvent is a mixture of water and the alcohol solvent.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacy, and specifically relates to a preparation method for rapidly separating and purifying crocin-1 from plants, and the use of crocin-1 in the field of pharmacy. Background technique [0002] Saffron is the upper part and stigma of the saffron (Crocus sativus L.) style of the Iridaceae plant. Originally produced in Spain, Iran, India and other countries, it was introduced into Tibet via India and then spread to my country. However, the yield of saffron style is extremely low, and generally only 1g of dry stigma can be obtained per 90 flowers, so the price is expensive, and it has the reputation of "plant gold". Drugs and the like are planted in Zhejiang and other places in China. In 2018, saffron was included in the "New Zhejiang Bawei", so it is of great significance to develop its function. Traditional Chinese medicine believes that saffron is sweet and slightly sour, flat in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/06C07H1/08A61P25/24A61P25/00
CPCC07H13/06C07H1/08A61P25/24A61P25/00
Inventor 王平林素素江瑜
Owner ZHEJIANG UNIV OF TECH
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