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Method for simultaneous extraction of hericium mushroom polysaccharides and proteins

A technique for synchronous extraction of Hericium erinaceus polysaccharides, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of high temperature, slow speed, and large damage to heat-sensitive components, and achieve high extraction rate and purity, The optimization of process parameters is conducive to the effect of popularization and application

Inactive Publication Date: 2019-10-18
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional extraction method has high temperature and slow speed, which will greatly damage the heat-sensitive components

Method used

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  • Method for simultaneous extraction of hericium mushroom polysaccharides and proteins
  • Method for simultaneous extraction of hericium mushroom polysaccharides and proteins
  • Method for simultaneous extraction of hericium mushroom polysaccharides and proteins

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Embodiment Construction

[0018] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0019] Simultaneous extraction process

[0020] Sample processing: Hericium erinaceus fruiting bodies were baked in a hot air drying oven at 40 °C for 16 h, pulverized by a grinder, and stored at -20 °C for later use.

[0021] Extraction process: Take 10 g Hericium erinaceus sample powder and add distilled water according to the ratio of liquid to material, use the flash method to extract under the set parameters, filter through filter cloth and centrifuge several times, adjust the pH value of the extract mixture of Hericium erinaceus polysaccharide and protein to The isoelectric point (pH 3.65), centrifuged to obtain the supernatant of crude polysaccharide and protein precipitate.

[0022] protein identification

[0023]...

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Abstract

The invention discloses a method for simultaneous extraction ofhericium mushroom polysaccharides and proteins. The method for the simultaneous extraction of the hericium mushroom polysaccharides and the proteins adopts a flash extraction method to extract the polysaccharides and the proteins from hericium mushroom jointly; a characteristic of protein aggregation near isoelectric points is exploited, an extracted protein mixture solution is precipitated by isoelectric points to realize the separation of the polysaccharides and the proteins,a technological parameter is optimized, extraction rateand purity of the polysaccharides and the proteins are high, and popularization and application are favorable.

Description

technical field [0001] The invention belongs to the technical field of effective component extraction, and in particular relates to a method for synchronously extracting polysaccharides and proteins from Hericium erinaceus. Background technique [0002] Hericium erinaceus ( Hericium erinaceus ) belongs to the order Basidiomycetes Hericium erinaceus, has significant curative effect on digestive system diseases, can effectively treat gastric ulcer, chronic gastritis and duodenal ulcer, and has a good inhibitory effect on gastric and intestinal tumors. Mountain delicacy Hericium, Seafood Shark's Fin". Hericium erinaceus is rich in polysaccharides, proteins, cellulose, and amino acids, including 8 kinds of essential amino acids for human body. Hericium erinaceus polysaccharides have a protective effect on the gastric mucosa, can promote digestion, and also have the effects of improving immunity, anti-aging, and lowering blood sugar. The common extraction methods of Hericium e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07K1/30
CPCC08B37/006C08B37/0003C07K1/30
Inventor 陈雷黄群张雄黄翔杨燃安凤平宋洪波
Owner FUJIAN AGRI & FORESTRY UNIV
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